Molecular cloning of equine preprorelaxin cDNA

Kwan Sik Min, Kunio Shiota, Tomoya Ogawa

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

A cDNA clone encoding preprorelaxin was isolated from the equine placental cDNA library and its nucleotide sequence was determined. Analysis of the sequence revealed that the cDNA contained a polyadenylation site and an open reading frame encoding 182 amino acids, including a signal peptide of 25 amino acids, a B-chain of 28, a C-peptide of 109 and an A-chain of 20 amino acids. The nucleotide sequence of preprorelaxin shows homology with those of the pig (75.5%), monkey (67.4%), human (67.4%), rat (60.7%) and mouse (54.6%). The main differences between equine preprorelaxin and that of these other species were the insertion of a Leu residue at position 87 in the C-peptide and also the deletion of two residues from the C-peptide and A-chain. Northern blot analysis of mRNA obtained from pregnancy day 70 placenta showed that there were two different sizes of mRNA (1.0 and 1.8 kb).

Original languageEnglish
Pages (from-to)171-178
Number of pages8
JournalJournal of Reproduction and Development
Volume42
Issue number3
Publication statusPublished - 1996
Externally publishedYes

Fingerprint

molecular cloning
peptides
horses
amino acids
nucleotide sequences
signal peptide
placenta
cDNA libraries
Northern blotting
monkeys
open reading frames
sequence analysis
pregnancy
clones
swine
rats
mice

Keywords

  • Equine placenta
  • Relaxin cDNA

ASJC Scopus subject areas

  • Animal Science and Zoology

Cite this

Min, K. S., Shiota, K., & Ogawa, T. (1996). Molecular cloning of equine preprorelaxin cDNA. Journal of Reproduction and Development, 42(3), 171-178.

Molecular cloning of equine preprorelaxin cDNA. / Min, Kwan Sik; Shiota, Kunio; Ogawa, Tomoya.

In: Journal of Reproduction and Development, Vol. 42, No. 3, 1996, p. 171-178.

Research output: Contribution to journalArticle

Min, KS, Shiota, K & Ogawa, T 1996, 'Molecular cloning of equine preprorelaxin cDNA', Journal of Reproduction and Development, vol. 42, no. 3, pp. 171-178.
Min, Kwan Sik ; Shiota, Kunio ; Ogawa, Tomoya. / Molecular cloning of equine preprorelaxin cDNA. In: Journal of Reproduction and Development. 1996 ; Vol. 42, No. 3. pp. 171-178.
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