Molecular cloning of kisspeptin receptor genes (gpr54-1 and gpr54-2) and their expression profiles in the brain of a tropical damselfish during different gonadal stages

Satoshi Imamura, Sung Pyo Hur, Yuki Takeuchi, Selma Bouchekioua, Akihiro Takemura

    Research output: Contribution to journalArticle

    7 Citations (Scopus)

    Abstract

    The kisspeptin receptor (GPR54) mediates neuroendocrine control of kisspeptin in the brain and acts as a gateway for a pulsatile release of hypothalamic gonadotropin-releasing hormone. This study aimed to clone two GPR54 genes (gpr54-1 and gpr54-2) from the brain of the sapphire devil Chrysiptera cyanea, a tropical damselfish, and to study their involvement in reproduction. The partial sequences of the sapphire devil gpr54-1 cDNA (1059 bp) and gpr54-2 cDNA (1098 bp) each had an open reading frame encoding a protein of 353 and 366 amino acids, respectively, both of which had structural features of a G-protein-coupled receptor. The expression of gpr54-1 mRNA was observed in the diencephalon and telencephalon, and gpr54-2 mRNA was found in the optic tectum of sapphire devil. When gpr54-1 and gpr54-2 mRNA levels were examined in the brain of sapphire devil by real-time quantitative polymerase chain reaction (qPCR), they were found to increase during late vitellogenesis and post-spawning. Treatment of fish with estradiol-17β (Ε2) resulted in an increase in gpr54-1 and gpr54-2 expression in the brain of sapphire devil. Thus, kisspeptin receptors likely mediate the activity of kisspeptin in the brain and are involved in controlling reproductive events in a tropical damselfish.

    Original languageEnglish
    Pages (from-to)9-16
    Number of pages8
    JournalComparative biochemistry and physiology. Part A, Molecular & integrative physiology
    Volume203
    DOIs
    Publication statusPublished - 2017 Jan 1

    Fingerprint

    Kisspeptins
    Aluminum Oxide
    Cloning
    Molecular Cloning
    Brain
    Genes
    Messenger RNA
    Complementary DNA
    Vitellogenesis
    Pituitary Hormone-Releasing Hormones
    Diencephalon
    Telencephalon
    Superior Colliculi
    Polymerase chain reaction
    G-Protein-Coupled Receptors
    Gonadotropin-Releasing Hormone
    Fish
    Open Reading Frames
    Reproduction
    Real-Time Polymerase Chain Reaction

    Keywords

    • Coral reef
    • Damselfish
    • Kisspeptin
    • Real-time PCR
    • Sapphire devil
    • Sex steroids
    • Vitellogenesis

    ASJC Scopus subject areas

    • Biochemistry
    • Molecular Biology
    • Physiology

    Cite this

    Molecular cloning of kisspeptin receptor genes (gpr54-1 and gpr54-2) and their expression profiles in the brain of a tropical damselfish during different gonadal stages. / Imamura, Satoshi; Hur, Sung Pyo; Takeuchi, Yuki; Bouchekioua, Selma; Takemura, Akihiro.

    In: Comparative biochemistry and physiology. Part A, Molecular & integrative physiology, Vol. 203, 01.01.2017, p. 9-16.

    Research output: Contribution to journalArticle

    @article{0ff1fcbf42ca4ac5bbf4ebc2fada6fdc,
    title = "Molecular cloning of kisspeptin receptor genes (gpr54-1 and gpr54-2) and their expression profiles in the brain of a tropical damselfish during different gonadal stages",
    abstract = "The kisspeptin receptor (GPR54) mediates neuroendocrine control of kisspeptin in the brain and acts as a gateway for a pulsatile release of hypothalamic gonadotropin-releasing hormone. This study aimed to clone two GPR54 genes (gpr54-1 and gpr54-2) from the brain of the sapphire devil Chrysiptera cyanea, a tropical damselfish, and to study their involvement in reproduction. The partial sequences of the sapphire devil gpr54-1 cDNA (1059 bp) and gpr54-2 cDNA (1098 bp) each had an open reading frame encoding a protein of 353 and 366 amino acids, respectively, both of which had structural features of a G-protein-coupled receptor. The expression of gpr54-1 mRNA was observed in the diencephalon and telencephalon, and gpr54-2 mRNA was found in the optic tectum of sapphire devil. When gpr54-1 and gpr54-2 mRNA levels were examined in the brain of sapphire devil by real-time quantitative polymerase chain reaction (qPCR), they were found to increase during late vitellogenesis and post-spawning. Treatment of fish with estradiol-17β (Ε2) resulted in an increase in gpr54-1 and gpr54-2 expression in the brain of sapphire devil. Thus, kisspeptin receptors likely mediate the activity of kisspeptin in the brain and are involved in controlling reproductive events in a tropical damselfish.",
    keywords = "Coral reef, Damselfish, Kisspeptin, Real-time PCR, Sapphire devil, Sex steroids, Vitellogenesis",
    author = "Satoshi Imamura and Hur, {Sung Pyo} and Yuki Takeuchi and Selma Bouchekioua and Akihiro Takemura",
    year = "2017",
    month = "1",
    day = "1",
    doi = "10.1016/j.cbpa.2016.07.015",
    language = "English",
    volume = "203",
    pages = "9--16",
    journal = "Comparative Biochemistry and Physiology - A Physiology",
    issn = "1095-6433",
    publisher = "Elsevier Inc.",

    }

    TY - JOUR

    T1 - Molecular cloning of kisspeptin receptor genes (gpr54-1 and gpr54-2) and their expression profiles in the brain of a tropical damselfish during different gonadal stages

    AU - Imamura, Satoshi

    AU - Hur, Sung Pyo

    AU - Takeuchi, Yuki

    AU - Bouchekioua, Selma

    AU - Takemura, Akihiro

    PY - 2017/1/1

    Y1 - 2017/1/1

    N2 - The kisspeptin receptor (GPR54) mediates neuroendocrine control of kisspeptin in the brain and acts as a gateway for a pulsatile release of hypothalamic gonadotropin-releasing hormone. This study aimed to clone two GPR54 genes (gpr54-1 and gpr54-2) from the brain of the sapphire devil Chrysiptera cyanea, a tropical damselfish, and to study their involvement in reproduction. The partial sequences of the sapphire devil gpr54-1 cDNA (1059 bp) and gpr54-2 cDNA (1098 bp) each had an open reading frame encoding a protein of 353 and 366 amino acids, respectively, both of which had structural features of a G-protein-coupled receptor. The expression of gpr54-1 mRNA was observed in the diencephalon and telencephalon, and gpr54-2 mRNA was found in the optic tectum of sapphire devil. When gpr54-1 and gpr54-2 mRNA levels were examined in the brain of sapphire devil by real-time quantitative polymerase chain reaction (qPCR), they were found to increase during late vitellogenesis and post-spawning. Treatment of fish with estradiol-17β (Ε2) resulted in an increase in gpr54-1 and gpr54-2 expression in the brain of sapphire devil. Thus, kisspeptin receptors likely mediate the activity of kisspeptin in the brain and are involved in controlling reproductive events in a tropical damselfish.

    AB - The kisspeptin receptor (GPR54) mediates neuroendocrine control of kisspeptin in the brain and acts as a gateway for a pulsatile release of hypothalamic gonadotropin-releasing hormone. This study aimed to clone two GPR54 genes (gpr54-1 and gpr54-2) from the brain of the sapphire devil Chrysiptera cyanea, a tropical damselfish, and to study their involvement in reproduction. The partial sequences of the sapphire devil gpr54-1 cDNA (1059 bp) and gpr54-2 cDNA (1098 bp) each had an open reading frame encoding a protein of 353 and 366 amino acids, respectively, both of which had structural features of a G-protein-coupled receptor. The expression of gpr54-1 mRNA was observed in the diencephalon and telencephalon, and gpr54-2 mRNA was found in the optic tectum of sapphire devil. When gpr54-1 and gpr54-2 mRNA levels were examined in the brain of sapphire devil by real-time quantitative polymerase chain reaction (qPCR), they were found to increase during late vitellogenesis and post-spawning. Treatment of fish with estradiol-17β (Ε2) resulted in an increase in gpr54-1 and gpr54-2 expression in the brain of sapphire devil. Thus, kisspeptin receptors likely mediate the activity of kisspeptin in the brain and are involved in controlling reproductive events in a tropical damselfish.

    KW - Coral reef

    KW - Damselfish

    KW - Kisspeptin

    KW - Real-time PCR

    KW - Sapphire devil

    KW - Sex steroids

    KW - Vitellogenesis

    UR - http://www.scopus.com/inward/record.url?scp=84981285566&partnerID=8YFLogxK

    UR - http://www.scopus.com/inward/citedby.url?scp=84981285566&partnerID=8YFLogxK

    U2 - 10.1016/j.cbpa.2016.07.015

    DO - 10.1016/j.cbpa.2016.07.015

    M3 - Article

    VL - 203

    SP - 9

    EP - 16

    JO - Comparative Biochemistry and Physiology - A Physiology

    JF - Comparative Biochemistry and Physiology - A Physiology

    SN - 1095-6433

    ER -