Molecular cloning of newt sex pheromone precursor cDNAs

Evidence for the existence of species-specific forms of pheromones

T. Iwata, K. Umezawa, F. Toyoda, N. Takahashi, H. Matsukawa, K. Yamamoto, S. Miura, H. Hayashi, S. Kikuyama

    Research output: Contribution to journalArticle

    33 Citations (Scopus)

    Abstract

    Cloning of cDNA encoding a decapeptide pheromone (sodefrin) that attracts conspecific female newts was attempted. A cDNA clone encoding a protein consisting of 189 amino acid residues including a sodefrin sequence was isolated from a Cynops pyrrhogaster abdominal gland cDNA library. Likewise, a cDNA clone encoding a molecule comparable to the sodefrin precursor was obtained from a Cynops ensicauda abdominal gland cDNA library. This clone encoded a precursor protein of 192 amino acid residues, including a sodefrin-like peptide sequence with substitutions of two amino acid residues. This is the first report of a peptide pheromone precursor in vertebrates. Copyright (C) 1999 Federation of European Biochemical Societies.

    Original languageEnglish
    Pages (from-to)400-404
    Number of pages5
    JournalFEBS Letters
    Volume457
    Issue number3
    DOIs
    Publication statusPublished - 1999 Sep 3

    Fingerprint

    sodefrin
    Salamandridae
    Sex Attractants
    Cloning
    Pheromones
    Molecular Cloning
    Complementary DNA
    Clone Cells
    Gene Library
    Amino Acids
    Peptides
    Protein Precursors
    Amino Acid Substitution
    Vertebrates
    Organism Cloning
    Substitution reactions
    Molecules

    Keywords

    • cDNA cloning
    • Female newt attracting pheromone
    • Pheromone precursor protein
    • Sodefrin

    ASJC Scopus subject areas

    • Biochemistry
    • Biophysics
    • Molecular Biology

    Cite this

    Iwata, T., Umezawa, K., Toyoda, F., Takahashi, N., Matsukawa, H., Yamamoto, K., ... Kikuyama, S. (1999). Molecular cloning of newt sex pheromone precursor cDNAs: Evidence for the existence of species-specific forms of pheromones. FEBS Letters, 457(3), 400-404. https://doi.org/10.1016/S0014-5793(99)01062-5

    Molecular cloning of newt sex pheromone precursor cDNAs : Evidence for the existence of species-specific forms of pheromones. / Iwata, T.; Umezawa, K.; Toyoda, F.; Takahashi, N.; Matsukawa, H.; Yamamoto, K.; Miura, S.; Hayashi, H.; Kikuyama, S.

    In: FEBS Letters, Vol. 457, No. 3, 03.09.1999, p. 400-404.

    Research output: Contribution to journalArticle

    Iwata, T, Umezawa, K, Toyoda, F, Takahashi, N, Matsukawa, H, Yamamoto, K, Miura, S, Hayashi, H & Kikuyama, S 1999, 'Molecular cloning of newt sex pheromone precursor cDNAs: Evidence for the existence of species-specific forms of pheromones', FEBS Letters, vol. 457, no. 3, pp. 400-404. https://doi.org/10.1016/S0014-5793(99)01062-5
    Iwata, T. ; Umezawa, K. ; Toyoda, F. ; Takahashi, N. ; Matsukawa, H. ; Yamamoto, K. ; Miura, S. ; Hayashi, H. ; Kikuyama, S. / Molecular cloning of newt sex pheromone precursor cDNAs : Evidence for the existence of species-specific forms of pheromones. In: FEBS Letters. 1999 ; Vol. 457, No. 3. pp. 400-404.
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