Molecular cloning of rat leukemia inhibitory factor receptor α-chain gene and its expression during pregnancy

Jun ichi Aikawa, Sakae Ikeda-Naiki, Jun Ohgane, Kwan Sik Min, Takuya Imamura, Ken Sasai, Kunio Shiota, Tomoya Ogawa

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Leukemia inhibitory factor (LIF) is a secreted glycoprotein and a pluripotent growth factor that acts on diverse cell systems. LIF transmits its effects via binding to transmembrane receptors, of which both high- and low-affinity forms have been identified. In this study, we analyzed the structure and expression of rat LIF receptor α-chain (rLIFR α) cDNA. A full-length clone of the cDNA encoding the membrane-bound form of rLIFR α protein was prepared by a combination of LA-PCR and 5' RACE using DNA reverse-transcribed from total RNA isolated from the livers of day-12 and day-14 pregnant rats as templates. The nucleotide sequence of a full-length clone was determined and further confirmed by analysis of shorter DNA fragment prepared by PCR using pfu polymerase. The gene for rLIFR α encodes a 1093 amine acid residue protein. The rLIFR α protein shows a high degree of similarity to mouse and human LIF receptor α-chain protein (89% and 76% amino acid sequence identifies, respectively). Only one molecular species of mRNA for the rLIFR α gene was detected in the liver and placenta. rLIFR α was expressed in liver of both non-pregnant and pregnant rats. The level of mRNA for the rLIFR α gene in placenta was maximum on day 16 of pregnancy.

Original languageEnglish
Pages (from-to)266-276
Number of pages11
JournalBiochimica et Biophysica Acta - Gene Structure and Expression
Volume1353
Issue number3
DOIs
Publication statusPublished - 1997 Sep 12
Externally publishedYes

Fingerprint

OSM-LIF Receptors
Cloning
Molecular Cloning
Rats
Genes
Gene Expression
Pregnancy
Liver
Leukemia Inhibitory Factor
Placenta
Proteins
Complementary DNA
Clone Cells
Polymerase Chain Reaction
Messenger RNA
rat Lifr protein
DNA
Amines
Amino Acid Sequence
Intercellular Signaling Peptides and Proteins

Keywords

  • (Rat)
  • CDNA cloning
  • Liver
  • Northern blot analysis
  • Placenta
  • Polymerase chain reaction
  • Pregnancy
  • Rat LIF receptor α-chain (rLIFRα)

ASJC Scopus subject areas

  • Biochemistry
  • Genetics
  • Structural Biology
  • Biophysics

Cite this

Molecular cloning of rat leukemia inhibitory factor receptor α-chain gene and its expression during pregnancy. / Aikawa, Jun ichi; Ikeda-Naiki, Sakae; Ohgane, Jun; Min, Kwan Sik; Imamura, Takuya; Sasai, Ken; Shiota, Kunio; Ogawa, Tomoya.

In: Biochimica et Biophysica Acta - Gene Structure and Expression, Vol. 1353, No. 3, 12.09.1997, p. 266-276.

Research output: Contribution to journalArticle

Aikawa, Jun ichi ; Ikeda-Naiki, Sakae ; Ohgane, Jun ; Min, Kwan Sik ; Imamura, Takuya ; Sasai, Ken ; Shiota, Kunio ; Ogawa, Tomoya. / Molecular cloning of rat leukemia inhibitory factor receptor α-chain gene and its expression during pregnancy. In: Biochimica et Biophysica Acta - Gene Structure and Expression. 1997 ; Vol. 1353, No. 3. pp. 266-276.
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