Molecular cloning of xenopus activin type I receptor and the analysis of its expression during embryogenesis

Mariko Kondo, Kentaro Senba, Koichiro Shiokawa, Tadashi Yamamoto

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

We cloned cDNAs coding for the Xenopus counterparts of a type I activin receptor. The cDNA clones were predicted to encode 56kd proteins, namely XAR3 and XAR4 proteins. The two proteins are highly homologous to each other, showing 96% identity in the amino acid sequences. The GST-fused kinase domain of XAR3 autophosphorylated itself in vitro on threonine residues. The expression of XAR4 was found throughout embryogenesis, from oocytes to tailbud embryos, and also in adult tissues. By whole mount in situ hybridization, the XAR4 transcripts were detected in the animal half of blastulae and dorsally in gastrulae and neurulae. In tadpoles, the transcript was seen in the brain and around the otic vesicles. These results show that the activin type I receptor is expressed during Xenopus embryogenesis and suggest that the type I and II receptors are expressed together both temporally and spatially, supporting the idea that activin induces mesoderm in the embryo through activation of the two types of receptors.

Original languageEnglish
Pages (from-to)549-555
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume218
Issue number2
DOIs
Publication statusPublished - 1996 Jan 17
Externally publishedYes

Fingerprint

Type I Activin Receptors
Cloning
Molecular Cloning
Xenopus
Embryonic Development
Embryonic Structures
Complementary DNA
Blastula
Gastrula
Activins
Proteins
Mesoderm
Threonine
Oocytes
In Situ Hybridization
Ear
Larva
Amino Acid Sequence
Brain
Animals

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Molecular cloning of xenopus activin type I receptor and the analysis of its expression during embryogenesis. / Kondo, Mariko; Senba, Kentaro; Shiokawa, Koichiro; Yamamoto, Tadashi.

In: Biochemical and Biophysical Research Communications, Vol. 218, No. 2, 17.01.1996, p. 549-555.

Research output: Contribution to journalArticle

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