Abstract
We performed a molecular cloning of the glutamic oxaloacetic transaminase (GOT1) gene from R. rugosa, and determined its chromosomal location. This gene was reportedly localized near the sex-determining region of the ZW sex chromosomes in the frog Buergeria buergeri; however, the GOT1 gene was mapped to the distal end of chromosome 9 in R. rugosa using a GOT1 cDNA FISH probe. This was also the case when a 46.3kb genomic clone containing exon 8 and 9 and the 3'-neighboring region of the GOT1 gene, designated clone B, was used as probe. However, weak signals were also detected at the telomeric ends of other autosomes and the Z sex chromosome, and near the centromeric region of the W sex chromosome. To intensify the signals, we used eight internal fragments in clone B and applied them to chromosome mapping. Consequently, only two fragments containing repeated sequence blocks produced hybridization signals; those signals were observed on autosomes and ZW sex chromosomes. The 3'-neighboring region contained two types of repeated sequence elements: a 41bp element, designated 41-REL, localized to telomeric ends of autosomes and a 31bp element, designated 31-REL, localized to telomeric ends of all autosomes and the ZW sex chromosomes, and also near the centromere on the W long arm. The results collectively suggest that the two repeated sequence elements were independently amplified around the chromosomal telomeres in R. rugosa, indicating that they will be useful cytogenetic markers for studying karyotypic evolution-especially the W chromosome differentiation-in this species.
| Original language | English |
|---|---|
| Pages (from-to) | 222-231 |
| Number of pages | 10 |
| Journal | Journal of Experimental Zoology Part A: Ecological Genetics and Physiology |
| Volume | 315 A |
| Issue number | 4 |
| DOIs | |
| Publication status | Published - 2011 Apr 1 |
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ASJC Scopus subject areas
- Animal Science and Zoology
- Ecology, Evolution, Behavior and Systematics
- Genetics
- Physiology
- Molecular Biology
Cite this
Molecular cytogenetic characterization of telomere-specific repetitive DNA sequences in Rana rugosa. / Suda, Mari; Uno, Yoshinobu; Mori, Yumiko; Matsuda, Yoichi; Nakamura, Masahisa.
In: Journal of Experimental Zoology Part A: Ecological Genetics and Physiology, Vol. 315 A, No. 4, 01.04.2011, p. 222-231.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Molecular cytogenetic characterization of telomere-specific repetitive DNA sequences in Rana rugosa
AU - Suda, Mari
AU - Uno, Yoshinobu
AU - Mori, Yumiko
AU - Matsuda, Yoichi
AU - Nakamura, Masahisa
PY - 2011/4/1
Y1 - 2011/4/1
N2 - We performed a molecular cloning of the glutamic oxaloacetic transaminase (GOT1) gene from R. rugosa, and determined its chromosomal location. This gene was reportedly localized near the sex-determining region of the ZW sex chromosomes in the frog Buergeria buergeri; however, the GOT1 gene was mapped to the distal end of chromosome 9 in R. rugosa using a GOT1 cDNA FISH probe. This was also the case when a 46.3kb genomic clone containing exon 8 and 9 and the 3'-neighboring region of the GOT1 gene, designated clone B, was used as probe. However, weak signals were also detected at the telomeric ends of other autosomes and the Z sex chromosome, and near the centromeric region of the W sex chromosome. To intensify the signals, we used eight internal fragments in clone B and applied them to chromosome mapping. Consequently, only two fragments containing repeated sequence blocks produced hybridization signals; those signals were observed on autosomes and ZW sex chromosomes. The 3'-neighboring region contained two types of repeated sequence elements: a 41bp element, designated 41-REL, localized to telomeric ends of autosomes and a 31bp element, designated 31-REL, localized to telomeric ends of all autosomes and the ZW sex chromosomes, and also near the centromere on the W long arm. The results collectively suggest that the two repeated sequence elements were independently amplified around the chromosomal telomeres in R. rugosa, indicating that they will be useful cytogenetic markers for studying karyotypic evolution-especially the W chromosome differentiation-in this species.
AB - We performed a molecular cloning of the glutamic oxaloacetic transaminase (GOT1) gene from R. rugosa, and determined its chromosomal location. This gene was reportedly localized near the sex-determining region of the ZW sex chromosomes in the frog Buergeria buergeri; however, the GOT1 gene was mapped to the distal end of chromosome 9 in R. rugosa using a GOT1 cDNA FISH probe. This was also the case when a 46.3kb genomic clone containing exon 8 and 9 and the 3'-neighboring region of the GOT1 gene, designated clone B, was used as probe. However, weak signals were also detected at the telomeric ends of other autosomes and the Z sex chromosome, and near the centromeric region of the W sex chromosome. To intensify the signals, we used eight internal fragments in clone B and applied them to chromosome mapping. Consequently, only two fragments containing repeated sequence blocks produced hybridization signals; those signals were observed on autosomes and ZW sex chromosomes. The 3'-neighboring region contained two types of repeated sequence elements: a 41bp element, designated 41-REL, localized to telomeric ends of autosomes and a 31bp element, designated 31-REL, localized to telomeric ends of all autosomes and the ZW sex chromosomes, and also near the centromere on the W long arm. The results collectively suggest that the two repeated sequence elements were independently amplified around the chromosomal telomeres in R. rugosa, indicating that they will be useful cytogenetic markers for studying karyotypic evolution-especially the W chromosome differentiation-in this species.
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U2 - 10.1002/jez.668
DO - 10.1002/jez.668
M3 - Article
C2 - 21328702
AN - SCOPUS:79953194089
VL - 315 A
SP - 222
EP - 231
JO - Journal of Experimental Zoology Part A: Ecological Genetics and Physiology
JF - Journal of Experimental Zoology Part A: Ecological Genetics and Physiology
SN - 1932-5223
IS - 4
ER -