Molecular cytogenetic characterization of telomere-specific repetitive DNA sequences in Rana rugosa

Mari Suda, Yoshinobu Uno, Yumiko Mori, Yoichi Matsuda, Masahisa Nakamura

    Research output: Contribution to journalArticle

    4 Citations (Scopus)

    Abstract

    We performed a molecular cloning of the glutamic oxaloacetic transaminase (GOT1) gene from R. rugosa, and determined its chromosomal location. This gene was reportedly localized near the sex-determining region of the ZW sex chromosomes in the frog Buergeria buergeri; however, the GOT1 gene was mapped to the distal end of chromosome 9 in R. rugosa using a GOT1 cDNA FISH probe. This was also the case when a 46.3kb genomic clone containing exon 8 and 9 and the 3'-neighboring region of the GOT1 gene, designated clone B, was used as probe. However, weak signals were also detected at the telomeric ends of other autosomes and the Z sex chromosome, and near the centromeric region of the W sex chromosome. To intensify the signals, we used eight internal fragments in clone B and applied them to chromosome mapping. Consequently, only two fragments containing repeated sequence blocks produced hybridization signals; those signals were observed on autosomes and ZW sex chromosomes. The 3'-neighboring region contained two types of repeated sequence elements: a 41bp element, designated 41-REL, localized to telomeric ends of autosomes and a 31bp element, designated 31-REL, localized to telomeric ends of all autosomes and the ZW sex chromosomes, and also near the centromere on the W long arm. The results collectively suggest that the two repeated sequence elements were independently amplified around the chromosomal telomeres in R. rugosa, indicating that they will be useful cytogenetic markers for studying karyotypic evolution-especially the W chromosome differentiation-in this species.

    Original languageEnglish
    Pages (from-to)222-231
    Number of pages10
    JournalJournal of Experimental Zoology Part A: Ecological Genetics and Physiology
    Volume315 A
    Issue number4
    DOIs
    Publication statusPublished - 2011 Apr 1

    Fingerprint

    Ranidae
    Sex Chromosomes
    Nucleic Acid Repetitive Sequences
    cytogenetics
    telomeres
    Telomere
    sex chromosomes
    Cytogenetics
    autosomes
    chromosome
    nucleotide sequences
    DNA
    W chromosome
    Clone Cells
    clones
    clone
    genes
    gene
    Genes
    Z chromosome

    ASJC Scopus subject areas

    • Animal Science and Zoology
    • Ecology, Evolution, Behavior and Systematics
    • Genetics
    • Physiology
    • Molecular Biology

    Cite this

    Molecular cytogenetic characterization of telomere-specific repetitive DNA sequences in Rana rugosa. / Suda, Mari; Uno, Yoshinobu; Mori, Yumiko; Matsuda, Yoichi; Nakamura, Masahisa.

    In: Journal of Experimental Zoology Part A: Ecological Genetics and Physiology, Vol. 315 A, No. 4, 01.04.2011, p. 222-231.

    Research output: Contribution to journalArticle

    Suda, Mari ; Uno, Yoshinobu ; Mori, Yumiko ; Matsuda, Yoichi ; Nakamura, Masahisa. / Molecular cytogenetic characterization of telomere-specific repetitive DNA sequences in Rana rugosa. In: Journal of Experimental Zoology Part A: Ecological Genetics and Physiology. 2011 ; Vol. 315 A, No. 4. pp. 222-231.
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