Molecular detection of epiphytic Acaryochloris spp. on marine macroalgae

Satoshi Ohkubo; Hideaki Miyashita; Akio Murakami; Haruko Takeyama; Tohru Tsuchiya; Mamoru Mimuro

doi:10.1128/AEM.01148-06

Molecular detection of epiphytic Acaryochloris spp. on marine macroalgae

Satoshi Ohkubo, Hideaki Miyashita^*, Akio Murakami, Haruko Takeyama, Tohru Tsuchiya, Mamoru Mimuro

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

35 Citations (Scopus)

Abstract

A molecular method for detecting the epiphyte community on marine macroalgae was developed by using PCR-denaturing gradient gel electrophoresis. Selective amplification of 16S rRNA gene fragments from either cyanobacteria or algal plastids improved the detection of minor epiphytes. Two phylotypes of Acaryochloris, a chlorophyll d-containing cyanobacterium, were found not only on red macroalgae but also on green and brown macroalgae.

Original language	English
Pages (from-to)	7912-7915
Number of pages	4
Journal	Applied and Environmental Microbiology
Volume	72
Issue number	12
DOIs	https://doi.org/10.1128/AEM.01148-06
Publication status	Published - 2006 Dec
Externally published	Yes

ASJC Scopus subject areas

Biotechnology
Food Science
Applied Microbiology and Biotechnology
Ecology

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abstract = "A molecular method for detecting the epiphyte community on marine macroalgae was developed by using PCR-denaturing gradient gel electrophoresis. Selective amplification of 16S rRNA gene fragments from either cyanobacteria or algal plastids improved the detection of minor epiphytes. Two phylotypes of Acaryochloris, a chlorophyll d-containing cyanobacterium, were found not only on red macroalgae but also on green and brown macroalgae.",

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AU - Ohkubo, Satoshi

AU - Miyashita, Hideaki

AU - Murakami, Akio

AU - Takeyama, Haruko

AU - Tsuchiya, Tohru

AU - Mimuro, Mamoru

PY - 2006/12

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AB - A molecular method for detecting the epiphyte community on marine macroalgae was developed by using PCR-denaturing gradient gel electrophoresis. Selective amplification of 16S rRNA gene fragments from either cyanobacteria or algal plastids improved the detection of minor epiphytes. Two phylotypes of Acaryochloris, a chlorophyll d-containing cyanobacterium, were found not only on red macroalgae but also on green and brown macroalgae.

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JO - Applied and Environmental Microbiology

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Molecular detection of epiphytic Acaryochloris spp. on marine macroalgae

Abstract

ASJC Scopus subject areas

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