Molecular detection of epiphytic Acaryochloris spp. on marine macroalgae

Satoshi Ohkubo, Hideaki Miyashita, Akio Murakami, Haruko Takeyama, Tohru Tsuchiya, Mamoru Mimuro

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

A molecular method for detecting the epiphyte community on marine macroalgae was developed by using PCR-denaturing gradient gel electrophoresis. Selective amplification of 16S rRNA gene fragments from either cyanobacteria or algal plastids improved the detection of minor epiphytes. Two phylotypes of Acaryochloris, a chlorophyll d-containing cyanobacterium, were found not only on red macroalgae but also on green and brown macroalgae.

Original languageEnglish
Pages (from-to)7912-7915
Number of pages4
JournalApplied and Environmental Microbiology
Volume72
Issue number12
DOIs
Publication statusPublished - 2006 Dec
Externally publishedYes

Fingerprint

Seaweed
macroalgae
cyanobacterium
Cyanobacteria
epiphyte
plastid
amplification
electrokinesis
Denaturing Gradient Gel Electrophoresis
chlorophyll
Plastids
gel
epiphytes
denaturing gradient gel electrophoresis
rRNA Genes
plastids
gene
ribosomal RNA
Polymerase Chain Reaction
detection

ASJC Scopus subject areas

  • Environmental Science(all)
  • Biotechnology
  • Microbiology

Cite this

Molecular detection of epiphytic Acaryochloris spp. on marine macroalgae. / Ohkubo, Satoshi; Miyashita, Hideaki; Murakami, Akio; Takeyama, Haruko; Tsuchiya, Tohru; Mimuro, Mamoru.

In: Applied and Environmental Microbiology, Vol. 72, No. 12, 12.2006, p. 7912-7915.

Research output: Contribution to journalArticle

Ohkubo, S, Miyashita, H, Murakami, A, Takeyama, H, Tsuchiya, T & Mimuro, M 2006, 'Molecular detection of epiphytic Acaryochloris spp. on marine macroalgae', Applied and Environmental Microbiology, vol. 72, no. 12, pp. 7912-7915. https://doi.org/10.1128/AEM.01148-06
Ohkubo, Satoshi ; Miyashita, Hideaki ; Murakami, Akio ; Takeyama, Haruko ; Tsuchiya, Tohru ; Mimuro, Mamoru. / Molecular detection of epiphytic Acaryochloris spp. on marine macroalgae. In: Applied and Environmental Microbiology. 2006 ; Vol. 72, No. 12. pp. 7912-7915.
@article{c2e0c8fe80ba4bca8f6c1e4cafee4ce3,
title = "Molecular detection of epiphytic Acaryochloris spp. on marine macroalgae",
abstract = "A molecular method for detecting the epiphyte community on marine macroalgae was developed by using PCR-denaturing gradient gel electrophoresis. Selective amplification of 16S rRNA gene fragments from either cyanobacteria or algal plastids improved the detection of minor epiphytes. Two phylotypes of Acaryochloris, a chlorophyll d-containing cyanobacterium, were found not only on red macroalgae but also on green and brown macroalgae.",
author = "Satoshi Ohkubo and Hideaki Miyashita and Akio Murakami and Haruko Takeyama and Tohru Tsuchiya and Mamoru Mimuro",
year = "2006",
month = "12",
doi = "10.1128/AEM.01148-06",
language = "English",
volume = "72",
pages = "7912--7915",
journal = "Applied and Environmental Microbiology",
issn = "0099-2240",
publisher = "American Society for Microbiology",
number = "12",

}

TY - JOUR

T1 - Molecular detection of epiphytic Acaryochloris spp. on marine macroalgae

AU - Ohkubo, Satoshi

AU - Miyashita, Hideaki

AU - Murakami, Akio

AU - Takeyama, Haruko

AU - Tsuchiya, Tohru

AU - Mimuro, Mamoru

PY - 2006/12

Y1 - 2006/12

N2 - A molecular method for detecting the epiphyte community on marine macroalgae was developed by using PCR-denaturing gradient gel electrophoresis. Selective amplification of 16S rRNA gene fragments from either cyanobacteria or algal plastids improved the detection of minor epiphytes. Two phylotypes of Acaryochloris, a chlorophyll d-containing cyanobacterium, were found not only on red macroalgae but also on green and brown macroalgae.

AB - A molecular method for detecting the epiphyte community on marine macroalgae was developed by using PCR-denaturing gradient gel electrophoresis. Selective amplification of 16S rRNA gene fragments from either cyanobacteria or algal plastids improved the detection of minor epiphytes. Two phylotypes of Acaryochloris, a chlorophyll d-containing cyanobacterium, were found not only on red macroalgae but also on green and brown macroalgae.

UR - http://www.scopus.com/inward/record.url?scp=33845522443&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33845522443&partnerID=8YFLogxK

U2 - 10.1128/AEM.01148-06

DO - 10.1128/AEM.01148-06

M3 - Article

VL - 72

SP - 7912

EP - 7915

JO - Applied and Environmental Microbiology

JF - Applied and Environmental Microbiology

SN - 0099-2240

IS - 12

ER -