Molecular diversity of bacterial chitinases in arable soils and the effects of environmental factors on the chitinolytic bacterial community

Takeshi Terahara, Seishi Ikeda, Chiaki Noritake, Kiwamu Minamisawa, Katsuhiko Ando, Satoshi Tsuneda, Shigeaki Harayama

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

The molecular diversity of bacterial chitinases in the bulk soils of arable land was investigated using culture-independent methods. The results demonstrate that bacterial chitinases in arable soils are highly diverse and comprise unique groups when their sequences were compared to those in public databases. The diversity of bacterial chitinases in arable soil was further evaluated using conventional phylogenetic analysis, the UniFrac analysis of the phylogenetic data, and the multidimensional scaling (MDS) analysis of T-RFLP profiles to elucidate the relationship between the diversity of bacterial chitinases and soil characteristics. These analyses indicate that environmental factors such as soil type and pH are responsible for shaping the composition of bacterial chitinases.

Original languageEnglish
Pages (from-to)473-480
Number of pages8
JournalSoil Biology and Biochemistry
Volume41
Issue number3
DOIs
Publication statusPublished - 2009 Mar 1

Keywords

  • Arable land
  • Canonical correspondence analysis
  • Chitinase
  • Multidimensional scaling analysis
  • Phylogenetic analysis
  • UniFrac analysis

ASJC Scopus subject areas

  • Microbiology
  • Soil Science

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