Molecular diversity of bacterial chitinases in arable soils and the effects of environmental factors on the chitinolytic bacterial community

Takeshi Terahara, Seishi Ikeda, Chiaki Noritake, Kiwamu Minamisawa, Katsuhiko Ando, Satoshi Tsuneda, Shigeaki Harayama

    Research output: Contribution to journalArticle

    33 Citations (Scopus)

    Abstract

    The molecular diversity of bacterial chitinases in the bulk soils of arable land was investigated using culture-independent methods. The results demonstrate that bacterial chitinases in arable soils are highly diverse and comprise unique groups when their sequences were compared to those in public databases. The diversity of bacterial chitinases in arable soil was further evaluated using conventional phylogenetic analysis, the UniFrac analysis of the phylogenetic data, and the multidimensional scaling (MDS) analysis of T-RFLP profiles to elucidate the relationship between the diversity of bacterial chitinases and soil characteristics. These analyses indicate that environmental factors such as soil type and pH are responsible for shaping the composition of bacterial chitinases.

    Original languageEnglish
    Pages (from-to)473-480
    Number of pages8
    JournalSoil Biology and Biochemistry
    Volume41
    Issue number3
    DOIs
    Publication statusPublished - 2009 Mar

    Fingerprint

    Chitinases
    chitinase
    arable soils
    bacterial communities
    environmental factor
    Soil
    environmental factors
    soil
    phylogenetics
    arable land
    phylogeny
    soil type
    Restriction Fragment Length Polymorphisms
    soil pH
    soil types
    effect
    Databases
    analysis

    Keywords

    • Arable land
    • Canonical correspondence analysis
    • Chitinase
    • Multidimensional scaling analysis
    • Phylogenetic analysis
    • UniFrac analysis

    ASJC Scopus subject areas

    • Soil Science
    • Microbiology

    Cite this

    Molecular diversity of bacterial chitinases in arable soils and the effects of environmental factors on the chitinolytic bacterial community. / Terahara, Takeshi; Ikeda, Seishi; Noritake, Chiaki; Minamisawa, Kiwamu; Ando, Katsuhiko; Tsuneda, Satoshi; Harayama, Shigeaki.

    In: Soil Biology and Biochemistry, Vol. 41, No. 3, 03.2009, p. 473-480.

    Research output: Contribution to journalArticle

    Terahara, Takeshi ; Ikeda, Seishi ; Noritake, Chiaki ; Minamisawa, Kiwamu ; Ando, Katsuhiko ; Tsuneda, Satoshi ; Harayama, Shigeaki. / Molecular diversity of bacterial chitinases in arable soils and the effects of environmental factors on the chitinolytic bacterial community. In: Soil Biology and Biochemistry. 2009 ; Vol. 41, No. 3. pp. 473-480.
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