Molecular evolution of Kiss2 genes and peptides in vertebrates

Tomohiro Osugi, Naohito Ohtaki, Yuya Sunakawa, You Lee Son, Makoto Ohkubo, Masayuki Iigo, Masafumi Amano, Kazuyoshi Tsutsui

    Research output: Contribution to journalArticle

    14 Citations (Scopus)

    Abstract

    The kiss1 peptide (kisspeptin), a product of the kiss1 gene, is one of the key neuropeptides regulating vertebrate reproduction. In 2009, we identified a paralogous gene of kiss1 in the brain of amphibians and named it kiss2. Currently, the presence of the kiss2 gene and the kiss2 peptide is still obscure in amniotes compared with that in other vertebrates. Therefore, we performed genomedatabase analyses in primates and reptiles to investigate the molecular evolution of the kiss2 gene in vertebrates. Because the mature kiss2 peptide has been identified only in amphibians, we further performed immunoaffinity purification and mass spectrometry to identify the mature endogenous kiss2 peptide in the brains of salmon and turtle that possessed the kiss2 gene. Here we provide the first evidence for the presence of a kiss2-like gene in the genome database of primates including humans. Synthetic amidated human KISS2 peptide activated human GPR54 expressed in COS7 cells, but nonamidated KISS2 peptide was inactive. The endogenous amidated kiss2 peptide may not be produced in primates because of the lack of an amidation signal in the precursor polypeptide. The kiss2-like gene may be nonfunctional in crocodilians because of premature stop codons. We identified the mature amidated kiss2 peptide in turtles and fish and analyzed the localization of kiss2 peptidemRNAexpression in fish. The present study suggests that the kiss2 gene may have mutated in primates and crocodilians and been lost in birds during the course of evolution. In contrast, the kiss2 gene and mature kiss2 peptide are present in turtles and fish.

    Original languageEnglish
    Pages (from-to)4270-4280
    Number of pages11
    JournalEndocrinology
    Volume154
    Issue number11
    DOIs
    Publication statusPublished - 2013 Nov 1

    Fingerprint

    Molecular Evolution
    Vertebrates
    Peptides
    Genes
    Primates
    Turtles
    Fishes
    Amphibians
    Kisspeptins
    Reptiles
    Nonsense Codon
    Salmon
    Brain
    Neuropeptides
    Birds
    Reproduction
    Mass Spectrometry
    Genome
    Databases

    ASJC Scopus subject areas

    • Endocrinology

    Cite this

    Osugi, T., Ohtaki, N., Sunakawa, Y., Son, Y. L., Ohkubo, M., Iigo, M., ... Tsutsui, K. (2013). Molecular evolution of Kiss2 genes and peptides in vertebrates. Endocrinology, 154(11), 4270-4280. https://doi.org/10.1210/en.2012-2267

    Molecular evolution of Kiss2 genes and peptides in vertebrates. / Osugi, Tomohiro; Ohtaki, Naohito; Sunakawa, Yuya; Son, You Lee; Ohkubo, Makoto; Iigo, Masayuki; Amano, Masafumi; Tsutsui, Kazuyoshi.

    In: Endocrinology, Vol. 154, No. 11, 01.11.2013, p. 4270-4280.

    Research output: Contribution to journalArticle

    Osugi, T, Ohtaki, N, Sunakawa, Y, Son, YL, Ohkubo, M, Iigo, M, Amano, M & Tsutsui, K 2013, 'Molecular evolution of Kiss2 genes and peptides in vertebrates', Endocrinology, vol. 154, no. 11, pp. 4270-4280. https://doi.org/10.1210/en.2012-2267
    Osugi T, Ohtaki N, Sunakawa Y, Son YL, Ohkubo M, Iigo M et al. Molecular evolution of Kiss2 genes and peptides in vertebrates. Endocrinology. 2013 Nov 1;154(11):4270-4280. https://doi.org/10.1210/en.2012-2267
    Osugi, Tomohiro ; Ohtaki, Naohito ; Sunakawa, Yuya ; Son, You Lee ; Ohkubo, Makoto ; Iigo, Masayuki ; Amano, Masafumi ; Tsutsui, Kazuyoshi. / Molecular evolution of Kiss2 genes and peptides in vertebrates. In: Endocrinology. 2013 ; Vol. 154, No. 11. pp. 4270-4280.
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