Mollusc gonadotropin-releasing hormone directly regulates gonadal functions: A primitive endocrine system controlling reproduction

Nicholas Treen, Naoki Itoh, Hanae Miura, Ippei Kikuchi, Takenori Ueda, Keisuke G. Takahashi, Takayoshi Ubuka, Kazutoshi Yamamoto, Peter J. Sharp, Kazuyoshi Tsutsui, Makoto Osada

    Research output: Contribution to journalArticle

    38 Citations (Scopus)

    Abstract

    Gonadotropin-releasing hormone (GnRH) is central to the control of vertebrate reproductive cycles and since GnRH orthologs are also present in invertebrates, it is likely that the common ancestor of bilateral animals possessed a GnRH-like peptide. In order to understand the evolutionary and comparative biology of GnRH peptides we cloned the cDNA transcripts of prepro GnRH-like peptides from two species of bivalve molluscs, the Yesso scallop Patinopecten yessoensis and the Pacific oyster Crassostrea gigas. We compared their deduced uncleaved and mature amino acid sequences with those from other invertebrates and vertebrates, and determined their sites of expression and biological activity. The two molluscan GnRH sequences increased the number of known protostome GnRHs to six different forms, indicating the current classification of protostome GnRHs requires further revision. In both molluscs, RT-PCR analysis showed that the genes were highly expressed in nervous tissue with lower levels present in peripheral tissues including the gonads, while immunocytochemistry, using anti-octopus GnRH-like peptide, demonstrated the presence of GnRH-like peptide in neural tissue. Putative scallop GnRH-like peptide stimulated spermatogonial cell division in cultured scallop testis, but the scallop GnRH-like peptide did not stimulate LH release from cultured quail pituitary cells. This is the first report of the cloning of bivalve GnRH-like peptide genes and of molluscan GnRH-like peptides that are biologically active in molluscs, but not in a vertebrate.

    Original languageEnglish
    Pages (from-to)167-172
    Number of pages6
    JournalGeneral and Comparative Endocrinology
    Volume176
    Issue number2
    DOIs
    Publication statusPublished - 2012 Apr 1

    Keywords

    • Functional evolution
    • GnRH
    • LH release
    • Molluscs
    • Neuropeptides
    • Spermatogenesis

    ASJC Scopus subject areas

    • Endocrinology

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  • Cite this

    Treen, N., Itoh, N., Miura, H., Kikuchi, I., Ueda, T., Takahashi, K. G., Ubuka, T., Yamamoto, K., Sharp, P. J., Tsutsui, K., & Osada, M. (2012). Mollusc gonadotropin-releasing hormone directly regulates gonadal functions: A primitive endocrine system controlling reproduction. General and Comparative Endocrinology, 176(2), 167-172. https://doi.org/10.1016/j.ygcen.2012.01.008