Monitoring cellular events in living mast cells stimulated with an extremely small amount of fluid on a microchip

Tatsuya Munaka, Hirohisa Abe, Masaki Kanai, Takashi Sakamoto, Hiroaki Nakanishi, Tetsuji Yamaoka, Shuichi Shoji, Akira Murakami

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    4 Citations (Scopus)

    Abstract

    We successfully developed a measurement system for real-time analysis of cellular function using a newly designed microchip. This microchip was equipped with a micro cell incubation chamber (240 nl) and was stimulated by a very small amount of stimuli (as small as 24 nl). Using the microchip system, cultivation of mast cells was successfully carried out. Monitoring of the cellular events after stimulation with an extremely small amount of fluid on a microchip was performed. This system could be applicable for various types of cellular analysis including real-time monitoring of cellular response by stimulation.

    Original languageEnglish
    JournalJapanese Journal of Applied Physics, Part 2: Letters
    Volume45
    Issue number24-28
    DOIs
    Publication statusPublished - 2006 Jun 16

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    Keywords

    • μTAS
    • Cell stimulation
    • Cellular analysis
    • Real-time monitoring

    ASJC Scopus subject areas

    • Physics and Astronomy (miscellaneous)

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