Monitoring surface-assisted biomolecular assembly by means of evanescent-field-coupled waveguide-mode nanobiosensors

Subash C.B. Gopinath, Koichi Awazu, Makoto Fujimaki, Katsuaki Sugimoto, Yoshimichi Ohki, Tetsuro Komatsubara, Junji Tominaga, Penmetcha K.R. Kumar

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Biological self-assembly is a natural process that involves various biomolecules, and finding the missing partner in these interactions is crucial for a specific biological function. Previously, we showed that evanescent-field-coupled waveguide-mode sensor in conjunction with a SiO 2 waveguide, the surfaces which contain cylindrical nanometric holes produced by atomic bombardment, allowed us to detect efficiently the biomolecular interactions. In the present studies, we showed that the assembly of biomolecules can be monitored using the evanescent-field-coupled waveguide-mode biosensor and thus provide a methodology in monitoring assembly process in macromolecular machines while they are assembling.

Original languageEnglish
Pages (from-to)481-488
Number of pages8
JournalAnalytical and Bioanalytical Chemistry
Volume394
Issue number2
DOIs
Publication statusPublished - 2009 May 1

Keywords

  • Aptamer
  • Biomolecular assembly
  • Evanescent-field-coupled waveguide
  • Nanobiosensor
  • SiO

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry

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  • Cite this

    Gopinath, S. C. B., Awazu, K., Fujimaki, M., Sugimoto, K., Ohki, Y., Komatsubara, T., Tominaga, J., & Kumar, P. K. R. (2009). Monitoring surface-assisted biomolecular assembly by means of evanescent-field-coupled waveguide-mode nanobiosensors. Analytical and Bioanalytical Chemistry, 394(2), 481-488. https://doi.org/10.1007/s00216-009-2721-0