Monitoring surface-assisted biomolecular assembly by means of evanescent-field-coupled waveguide-mode nanobiosensors

Subash C B Gopinath, Koichi Awazu, Makoto Fujimaki, Katsuaki Sugimoto, Yoshimichi Ohki, Tetsuro Komatsubara, Junji Tominaga, Penmetcha K R Kumar

    Research output: Contribution to journalArticle

    16 Citations (Scopus)

    Abstract

    Biological self-assembly is a natural process that involves various biomolecules, and finding the missing partner in these interactions is crucial for a specific biological function. Previously, we showed that evanescent-field-coupled waveguide-mode sensor in conjunction with a SiO 2 waveguide, the surfaces which contain cylindrical nanometric holes produced by atomic bombardment, allowed us to detect efficiently the biomolecular interactions. In the present studies, we showed that the assembly of biomolecules can be monitored using the evanescent-field-coupled waveguide-mode biosensor and thus provide a methodology in monitoring assembly process in macromolecular machines while they are assembling.

    Original languageEnglish
    Pages (from-to)481-488
    Number of pages8
    JournalAnalytical and Bioanalytical Chemistry
    Volume394
    Issue number2
    DOIs
    Publication statusPublished - 2009 May

    Fingerprint

    Evanescent fields
    Biosensing Techniques
    Waveguides
    Biomolecules
    Monitoring
    Biosensors
    Self assembly
    Sensors

    Keywords

    • Aptamer
    • Biomolecular assembly
    • Evanescent-field-coupled waveguide
    • Nanobiosensor
    • SiO

    ASJC Scopus subject areas

    • Analytical Chemistry
    • Biochemistry

    Cite this

    Monitoring surface-assisted biomolecular assembly by means of evanescent-field-coupled waveguide-mode nanobiosensors. / Gopinath, Subash C B; Awazu, Koichi; Fujimaki, Makoto; Sugimoto, Katsuaki; Ohki, Yoshimichi; Komatsubara, Tetsuro; Tominaga, Junji; Kumar, Penmetcha K R.

    In: Analytical and Bioanalytical Chemistry, Vol. 394, No. 2, 05.2009, p. 481-488.

    Research output: Contribution to journalArticle

    Gopinath, Subash C B ; Awazu, Koichi ; Fujimaki, Makoto ; Sugimoto, Katsuaki ; Ohki, Yoshimichi ; Komatsubara, Tetsuro ; Tominaga, Junji ; Kumar, Penmetcha K R. / Monitoring surface-assisted biomolecular assembly by means of evanescent-field-coupled waveguide-mode nanobiosensors. In: Analytical and Bioanalytical Chemistry. 2009 ; Vol. 394, No. 2. pp. 481-488.
    @article{48a21ba079cb415d8fddd81d28e23677,
    title = "Monitoring surface-assisted biomolecular assembly by means of evanescent-field-coupled waveguide-mode nanobiosensors",
    abstract = "Biological self-assembly is a natural process that involves various biomolecules, and finding the missing partner in these interactions is crucial for a specific biological function. Previously, we showed that evanescent-field-coupled waveguide-mode sensor in conjunction with a SiO 2 waveguide, the surfaces which contain cylindrical nanometric holes produced by atomic bombardment, allowed us to detect efficiently the biomolecular interactions. In the present studies, we showed that the assembly of biomolecules can be monitored using the evanescent-field-coupled waveguide-mode biosensor and thus provide a methodology in monitoring assembly process in macromolecular machines while they are assembling.",
    keywords = "Aptamer, Biomolecular assembly, Evanescent-field-coupled waveguide, Nanobiosensor, SiO",
    author = "Gopinath, {Subash C B} and Koichi Awazu and Makoto Fujimaki and Katsuaki Sugimoto and Yoshimichi Ohki and Tetsuro Komatsubara and Junji Tominaga and Kumar, {Penmetcha K R}",
    year = "2009",
    month = "5",
    doi = "10.1007/s00216-009-2721-0",
    language = "English",
    volume = "394",
    pages = "481--488",
    journal = "Fresenius Zeitschrift fur Analytische Chemie",
    issn = "0016-1152",
    publisher = "Springer Verlag",
    number = "2",

    }

    TY - JOUR

    T1 - Monitoring surface-assisted biomolecular assembly by means of evanescent-field-coupled waveguide-mode nanobiosensors

    AU - Gopinath, Subash C B

    AU - Awazu, Koichi

    AU - Fujimaki, Makoto

    AU - Sugimoto, Katsuaki

    AU - Ohki, Yoshimichi

    AU - Komatsubara, Tetsuro

    AU - Tominaga, Junji

    AU - Kumar, Penmetcha K R

    PY - 2009/5

    Y1 - 2009/5

    N2 - Biological self-assembly is a natural process that involves various biomolecules, and finding the missing partner in these interactions is crucial for a specific biological function. Previously, we showed that evanescent-field-coupled waveguide-mode sensor in conjunction with a SiO 2 waveguide, the surfaces which contain cylindrical nanometric holes produced by atomic bombardment, allowed us to detect efficiently the biomolecular interactions. In the present studies, we showed that the assembly of biomolecules can be monitored using the evanescent-field-coupled waveguide-mode biosensor and thus provide a methodology in monitoring assembly process in macromolecular machines while they are assembling.

    AB - Biological self-assembly is a natural process that involves various biomolecules, and finding the missing partner in these interactions is crucial for a specific biological function. Previously, we showed that evanescent-field-coupled waveguide-mode sensor in conjunction with a SiO 2 waveguide, the surfaces which contain cylindrical nanometric holes produced by atomic bombardment, allowed us to detect efficiently the biomolecular interactions. In the present studies, we showed that the assembly of biomolecules can be monitored using the evanescent-field-coupled waveguide-mode biosensor and thus provide a methodology in monitoring assembly process in macromolecular machines while they are assembling.

    KW - Aptamer

    KW - Biomolecular assembly

    KW - Evanescent-field-coupled waveguide

    KW - Nanobiosensor

    KW - SiO

    UR - http://www.scopus.com/inward/record.url?scp=67349131608&partnerID=8YFLogxK

    UR - http://www.scopus.com/inward/citedby.url?scp=67349131608&partnerID=8YFLogxK

    U2 - 10.1007/s00216-009-2721-0

    DO - 10.1007/s00216-009-2721-0

    M3 - Article

    VL - 394

    SP - 481

    EP - 488

    JO - Fresenius Zeitschrift fur Analytische Chemie

    JF - Fresenius Zeitschrift fur Analytische Chemie

    SN - 0016-1152

    IS - 2

    ER -