Monitoring the microbial population dynamics at the start-up stage of wastewater treatment reactor by terminal restriction fragment length polymorphism analysis based on 16s rDNA and rRNA gene sequences

Takeshi Terahara, Tatsuhiko Hoshino, Satoshi Tsuneda, Akira Hirata, Yuhei Inamori

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

The microbial population dynamics at the start-up stage of a wastewater treatment reactor was investigated using terminal restriction fragment length polymorphism (T-RFLP) analysis based on 16S rDNA and rRNA gene sequences. The results of fragment peaks suggested that the number and activity of nitrifying bacteria increased in association with the start of nitrification, and the relative ratios of 16S rRNA of these bacteria changed prior to those of the 16S rDNA. Furthermore, multidimensional scaling (MDS) analysis revealed that the 16S rRNA exhibited wider dispersion than the 16S rDNA at the start-up stage, indicating that the diversity of 16S rRNA in the microbial communities was strongly affected by environmental changes.

Original languageEnglish
Pages (from-to)425-428
Number of pages4
JournalJournal of Bioscience and Bioengineering
Volume98
Issue number6
DOIs
Publication statusPublished - 2004 Dec

Keywords

  • Microbial population dynamics
  • Multidimensional scaling (MDS)
  • Start-up stage
  • Terminal restriction fragment length polymorphism (T-RFLP)
  • Wastewater treatment reactor

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

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