Monoclonal antibody analysis of phosphatidylserine and protein kinase C localizations in developing rat cerebellum

Atsuo Miyazawa, Hiroko Inoue, Tohru Yoshioka, Tetsuro Horikoshi, Keiji Yanagisawa, Masato Umeda, Keizo Inoue

    Research output: Contribution to journalArticle

    12 Citations (Scopus)

    Abstract

    Understanding the topographical relationships between phosphatidylserine (PS) and protein kinase C (PKC) within neurons can provide clues about the mechanism of translocation and activation of PKC. For this purpose we applied monoclonal antibodies (Abs) of PS and PKC to sections of developing rat cerebellum. The anti-PKC Ab immunohistochemical pattern showed homogeneous staining of Purkinje cells over various postnatal ages, whereas the anti-PS Ab staining showed a heterogeneous localization over these ages. Purkinje cells did not stain well between postnatal day 14 (PND 14) and PND 21, suggesting that the PS was lost from the membrane during preparation of the sections during this period. These data imply that interactions between PS and PKC vary in Purkinje cells during postnatal development.

    Original languageEnglish
    Pages (from-to)1547-1554
    Number of pages8
    JournalJournal of Neurochemistry
    Volume59
    Issue number4
    Publication statusPublished - 1992 Oct

    Fingerprint

    Phosphatidylserines
    Cerebellum
    Protein Kinase C
    Rats
    Monoclonal Antibodies
    Purkinje Cells
    Staining and Labeling
    Neurons
    Coloring Agents
    Chemical activation
    Membranes

    Keywords

    • Anti-phosphatidylserine antibody
    • Antiprotein kinase C antibody
    • Cerebellum
    • Developing Purkinje cell
    • Immunohistochemical study
    • Phosphatidylserine-protein kinase C interaction

    ASJC Scopus subject areas

    • Biochemistry
    • Cellular and Molecular Neuroscience

    Cite this

    Miyazawa, A., Inoue, H., Yoshioka, T., Horikoshi, T., Yanagisawa, K., Umeda, M., & Inoue, K. (1992). Monoclonal antibody analysis of phosphatidylserine and protein kinase C localizations in developing rat cerebellum. Journal of Neurochemistry, 59(4), 1547-1554.

    Monoclonal antibody analysis of phosphatidylserine and protein kinase C localizations in developing rat cerebellum. / Miyazawa, Atsuo; Inoue, Hiroko; Yoshioka, Tohru; Horikoshi, Tetsuro; Yanagisawa, Keiji; Umeda, Masato; Inoue, Keizo.

    In: Journal of Neurochemistry, Vol. 59, No. 4, 10.1992, p. 1547-1554.

    Research output: Contribution to journalArticle

    Miyazawa, A, Inoue, H, Yoshioka, T, Horikoshi, T, Yanagisawa, K, Umeda, M & Inoue, K 1992, 'Monoclonal antibody analysis of phosphatidylserine and protein kinase C localizations in developing rat cerebellum', Journal of Neurochemistry, vol. 59, no. 4, pp. 1547-1554.
    Miyazawa A, Inoue H, Yoshioka T, Horikoshi T, Yanagisawa K, Umeda M et al. Monoclonal antibody analysis of phosphatidylserine and protein kinase C localizations in developing rat cerebellum. Journal of Neurochemistry. 1992 Oct;59(4):1547-1554.
    Miyazawa, Atsuo ; Inoue, Hiroko ; Yoshioka, Tohru ; Horikoshi, Tetsuro ; Yanagisawa, Keiji ; Umeda, Masato ; Inoue, Keizo. / Monoclonal antibody analysis of phosphatidylserine and protein kinase C localizations in developing rat cerebellum. In: Journal of Neurochemistry. 1992 ; Vol. 59, No. 4. pp. 1547-1554.
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