Moonlight affects mRNA abundance of arylalkylamine N-acetyltransferase in the retina of a lunar-synchronized spawner, the goldlined spinefoot

Tomomi Kashiwagi, Yong Ju Park, Ji Gweon Park, Satoshi Imamura, Yuki Takeuchi, Sung Pyo Hur, Akihiro Takemura

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Melatonin synthesis in the pineal gland and retina shows a rhythmic fashion with high levels at night and is controlled by a rate-limiting enzyme, arylalkylamine N-acetyltransferase (AANAT). A previous study revealed that moonlight suppresses the plasma melatonin levels of the goldlined spinefoot (Siganus guttatus), which exhibits a lunar cycle in its reproductive activity and repeats gonadal development toward and spawning around the first quarter moon. Whether the retina of this species responds to moonlight is unknown. To clarify the photoperceptive ability of this species, we aimed to clone the full-length cDNA of Aanat1 (sgAanat1) from the retina and examine its transcriptional pattern under several daylight and moonlight regimes. The full-length sgAanat1 cDNA (1,038bp) contained a reading frame encoding a protein of 225 amino acids, which was highly homologous to AANAT1 of other teleosts. Reverse transcription-polymerase chain reaction (PCR) analysis revealed that among the tissues tested, sgAanat1 fragments were expressed exclusively in the retina. Real-time quantitative PCR analysis revealed that sgAanat1 fluctuated with high abundance at night under light-dark cycle and at subjective night under constant darkness, but not under constant light. These results suggest that sgAanat1 is regulated by both the external light signal and internal clock system. The abundance of sgAanat1 in the retina was higher at the culmination time around new moon than full moon phase. Additionally, exposing fish to brightness around the full moon period suppressed sgAanat1 mRNA abundance. Thus, moonlight is perceived by fish and has an impact on melatonin fluctuation in the retina.

Original languageEnglish
Pages (from-to)505-516
Number of pages12
JournalJournal of Experimental Zoology Part A: Ecological Genetics and Physiology
Volume319
Issue number9
DOIs
Publication statusPublished - 2013 Nov
Externally publishedYes

Fingerprint

Arylalkylamine N-Acetyltransferase
retina
Moon
Retina
Messenger RNA
polymerase chain reaction
melatonin
Melatonin
lunar cycle
lunar phase
fish
teleost
clone
Fishes
spawning
Complementary DNA
amino acid
enzyme
Siganus
Light

ASJC Scopus subject areas

  • Animal Science and Zoology
  • Ecology, Evolution, Behavior and Systematics
  • Genetics
  • Physiology
  • Molecular Biology

Cite this

Moonlight affects mRNA abundance of arylalkylamine N-acetyltransferase in the retina of a lunar-synchronized spawner, the goldlined spinefoot. / Kashiwagi, Tomomi; Park, Yong Ju; Park, Ji Gweon; Imamura, Satoshi; Takeuchi, Yuki; Hur, Sung Pyo; Takemura, Akihiro.

In: Journal of Experimental Zoology Part A: Ecological Genetics and Physiology, Vol. 319, No. 9, 11.2013, p. 505-516.

Research output: Contribution to journalArticle

Kashiwagi, Tomomi ; Park, Yong Ju ; Park, Ji Gweon ; Imamura, Satoshi ; Takeuchi, Yuki ; Hur, Sung Pyo ; Takemura, Akihiro. / Moonlight affects mRNA abundance of arylalkylamine N-acetyltransferase in the retina of a lunar-synchronized spawner, the goldlined spinefoot. In: Journal of Experimental Zoology Part A: Ecological Genetics and Physiology. 2013 ; Vol. 319, No. 9. pp. 505-516.
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