Morphology of C6 glioma cells on a water-repellent fractal alkyl ketene dimer surface

Wei Wei Hu, Hu Yan, Naoko Birukawa, Masashi Abeh, Etsuro Ito, Kaoru Tsuji, Zhong Chen, Akihisa Urano

Research output: Contribution to journalReview article

5 Citations (Scopus)

Abstract

In many in vitro cultures, cells may change their morphology, probably caused by adherence to the surface of the culture dish. Since a fractal alkyl ketene dimer (AKD) surface provides super water-repellency with a contact angle of 174°, we considered that it might provide an improved surface environment for the growth and differentiation of cells by preventing intimate adhesion. C6 glioma cells which were selected to test the effects of the fractal surface, were cultured on a conventional surface, a smooth AKD surface or a fractal AKD surface. On the conventional and smooth AKD surfaces, cells developed bipolar or multipolar shapes with enlarged cell bodies and neurite-like processes. In contrast, cells cultured on the fractal AKD surface presented fine filopodium-like processes like protoplasmic astrocytes in vivo, and higher morphological complexity was revealed by fractal analysis. Reconstruction of three-dimensional shape indicated that cells on the fractal surface were globular, whereas those on the conventional surface were rather flat. Our results suggest that C6 glioma cells on a fractal AKD surface show features of natural astrocytes with their elaborate morphology. The fractal surface thus may provide a new and natural culture enviromnent for experimental assessment of glial structure and function.

Original languageEnglish
Pages (from-to)224-231
Number of pages8
JournalCurrent Nanoscience
Volume4
Issue number3
DOIs
Publication statusPublished - 2008 Aug
Externally publishedYes

Fingerprint

Fractals
Glioma
Dimers
Water
Astrocytes
Pseudopodia
ketene
Cell Shape
Neurites
Neuroglia
Cells
Cell Differentiation
Cultured Cells
Cell Culture Techniques
Cell culture
Contact angle
Growth

Keywords

  • Alkyl ketene dimer (AKD)
  • C6 glioma cell
  • cAMP
  • Cytoskeleton
  • Fractal dimension

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Biomedical Engineering
  • Medicine (miscellaneous)
  • Pharmaceutical Science

Cite this

Morphology of C6 glioma cells on a water-repellent fractal alkyl ketene dimer surface. / Hu, Wei Wei; Yan, Hu; Birukawa, Naoko; Abeh, Masashi; Ito, Etsuro; Tsuji, Kaoru; Chen, Zhong; Urano, Akihisa.

In: Current Nanoscience, Vol. 4, No. 3, 08.2008, p. 224-231.

Research output: Contribution to journalReview article

Hu, WW, Yan, H, Birukawa, N, Abeh, M, Ito, E, Tsuji, K, Chen, Z & Urano, A 2008, 'Morphology of C6 glioma cells on a water-repellent fractal alkyl ketene dimer surface', Current Nanoscience, vol. 4, no. 3, pp. 224-231. https://doi.org/10.2174/157341308785161118
Hu, Wei Wei ; Yan, Hu ; Birukawa, Naoko ; Abeh, Masashi ; Ito, Etsuro ; Tsuji, Kaoru ; Chen, Zhong ; Urano, Akihisa. / Morphology of C6 glioma cells on a water-repellent fractal alkyl ketene dimer surface. In: Current Nanoscience. 2008 ; Vol. 4, No. 3. pp. 224-231.
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