Abstract
In many in vitro cultures, cells may change their morphology, probably caused by adherence to the surface of the culture dish. Since a fractal alkyl ketene dimer (AKD) surface provides super water-repellency with a contact angle of 174°, we considered that it might provide an improved surface environment for the growth and differentiation of cells by preventing intimate adhesion. C6 glioma cells which were selected to test the effects of the fractal surface, were cultured on a conventional surface, a smooth AKD surface or a fractal AKD surface. On the conventional and smooth AKD surfaces, cells developed bipolar or multipolar shapes with enlarged cell bodies and neurite-like processes. In contrast, cells cultured on the fractal AKD surface presented fine filopodium-like processes like protoplasmic astrocytes in vivo, and higher morphological complexity was revealed by fractal analysis. Reconstruction of three-dimensional shape indicated that cells on the fractal surface were globular, whereas those on the conventional surface were rather flat. Our results suggest that C6 glioma cells on a fractal AKD surface show features of natural astrocytes with their elaborate morphology. The fractal surface thus may provide a new and natural culture enviromnent for experimental assessment of glial structure and function.
| Original language | English |
|---|---|
| Pages (from-to) | 224-231 |
| Number of pages | 8 |
| Journal | Current Nanoscience |
| Volume | 4 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - 2008 Aug |
| Externally published | Yes |
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Keywords
- Alkyl ketene dimer (AKD)
- C6 glioma cell
- cAMP
- Cytoskeleton
- Fractal dimension
ASJC Scopus subject areas
- Biotechnology
- Bioengineering
- Biomedical Engineering
- Medicine (miscellaneous)
- Pharmaceutical Science
Cite this
Morphology of C6 glioma cells on a water-repellent fractal alkyl ketene dimer surface. / Hu, Wei Wei; Yan, Hu; Birukawa, Naoko; Abeh, Masashi; Ito, Etsuro; Tsuji, Kaoru; Chen, Zhong; Urano, Akihisa.
In: Current Nanoscience, Vol. 4, No. 3, 08.2008, p. 224-231.Research output: Contribution to journal › Review article
}
TY - JOUR
T1 - Morphology of C6 glioma cells on a water-repellent fractal alkyl ketene dimer surface
AU - Hu, Wei Wei
AU - Yan, Hu
AU - Birukawa, Naoko
AU - Abeh, Masashi
AU - Ito, Etsuro
AU - Tsuji, Kaoru
AU - Chen, Zhong
AU - Urano, Akihisa
PY - 2008/8
Y1 - 2008/8
N2 - In many in vitro cultures, cells may change their morphology, probably caused by adherence to the surface of the culture dish. Since a fractal alkyl ketene dimer (AKD) surface provides super water-repellency with a contact angle of 174°, we considered that it might provide an improved surface environment for the growth and differentiation of cells by preventing intimate adhesion. C6 glioma cells which were selected to test the effects of the fractal surface, were cultured on a conventional surface, a smooth AKD surface or a fractal AKD surface. On the conventional and smooth AKD surfaces, cells developed bipolar or multipolar shapes with enlarged cell bodies and neurite-like processes. In contrast, cells cultured on the fractal AKD surface presented fine filopodium-like processes like protoplasmic astrocytes in vivo, and higher morphological complexity was revealed by fractal analysis. Reconstruction of three-dimensional shape indicated that cells on the fractal surface were globular, whereas those on the conventional surface were rather flat. Our results suggest that C6 glioma cells on a fractal AKD surface show features of natural astrocytes with their elaborate morphology. The fractal surface thus may provide a new and natural culture enviromnent for experimental assessment of glial structure and function.
AB - In many in vitro cultures, cells may change their morphology, probably caused by adherence to the surface of the culture dish. Since a fractal alkyl ketene dimer (AKD) surface provides super water-repellency with a contact angle of 174°, we considered that it might provide an improved surface environment for the growth and differentiation of cells by preventing intimate adhesion. C6 glioma cells which were selected to test the effects of the fractal surface, were cultured on a conventional surface, a smooth AKD surface or a fractal AKD surface. On the conventional and smooth AKD surfaces, cells developed bipolar or multipolar shapes with enlarged cell bodies and neurite-like processes. In contrast, cells cultured on the fractal AKD surface presented fine filopodium-like processes like protoplasmic astrocytes in vivo, and higher morphological complexity was revealed by fractal analysis. Reconstruction of three-dimensional shape indicated that cells on the fractal surface were globular, whereas those on the conventional surface were rather flat. Our results suggest that C6 glioma cells on a fractal AKD surface show features of natural astrocytes with their elaborate morphology. The fractal surface thus may provide a new and natural culture enviromnent for experimental assessment of glial structure and function.
KW - Alkyl ketene dimer (AKD)
KW - C6 glioma cell
KW - cAMP
KW - Cytoskeleton
KW - Fractal dimension
UR - http://www.scopus.com/inward/record.url?scp=53649092027&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=53649092027&partnerID=8YFLogxK
U2 - 10.2174/157341308785161118
DO - 10.2174/157341308785161118
M3 - Review article
AN - SCOPUS:53649092027
VL - 4
SP - 224
EP - 231
JO - Current Nanoscience
JF - Current Nanoscience
SN - 1573-4137
IS - 3
ER -