Mouse-Musashi-1, a neural RNA-Binding protein highly enriched in the mammalian CNS stem cell

Shinichi Sakakibara, Takao Imai, Kayoko Hamaguchi, Masataka Okabe, Jun Aruga, Kazunori Nakajima, Daisuke Yasutomi, Takashi Nagata, Yasuyuki Kurihara, Seiichi Uesugi, Takaki Miyata, Masaharu Ogawa, Katsuhiko Mikoshiba, Hideyuki Okano

Research output: Contribution to journalArticle

426 Citations (Scopus)

Abstract

There is increasing interest in the role of RNA-binding proteins during neural development. Drosophila Musashi is one of the neural RNA-binding proteins essential for neural development and required for asymmetric cell divisions in the Drosophila adult sensory organ development. Here, a novel mammalian neural RNA-binding protein, mouse Musashi-1, was identified based on the homology to Drosophila Musashi and Xenopus NRP-1. In the developing CNS, mouse-Musashi-1 protein was highly enriched in the CNS stem cell. Single-cell culture experiments indicated that mouse Musashi-1 expression is associated with neural precursor cells that are capable of generating neurons and glia. In contrast, in fully differentiated neuronal and glial cells mouse-Musashi-1 expression is lost. This expression pattern of mouse-Musashi-1 is complementary to that of another mammalian neural RNA-binding protein, Hu (a mammalian homologue of a Drosophila neuronal RNA-binding protein Elav), that is expressed in postmitotic neurons within the CNS. In vitro studies indicated that mouse-Musashi-1 possesses binding preferences on poly(G) RNA homopolymer, whereas Hu is known to preferentially bind to short A/U-rich regions in RNA. Based on their differential expression patterns and distinct preferential target RNA sequences, we believe that the mouse-Musashi-1 and Hu proteins may play distinct roles in neurogenesis, either through sequential regulatory mechanisms or differential sorting of mRNA populations during asymmetric division of neural precursor cells.

Original languageEnglish
Pages (from-to)230-242
Number of pages13
JournalDevelopmental Biology
Volume176
Issue number2
DOIs
Publication statusPublished - 1996 Jun 15
Externally publishedYes

Fingerprint

RNA-Binding Proteins
Stem Cells
Drosophila
Neuroglia
RNA Transport
Poly G
Asymmetric Cell Division
RNA
Neurons
Neurogenesis
Xenopus
Cell Culture Techniques
Population

ASJC Scopus subject areas

  • Developmental Biology

Cite this

Mouse-Musashi-1, a neural RNA-Binding protein highly enriched in the mammalian CNS stem cell. / Sakakibara, Shinichi; Imai, Takao; Hamaguchi, Kayoko; Okabe, Masataka; Aruga, Jun; Nakajima, Kazunori; Yasutomi, Daisuke; Nagata, Takashi; Kurihara, Yasuyuki; Uesugi, Seiichi; Miyata, Takaki; Ogawa, Masaharu; Mikoshiba, Katsuhiko; Okano, Hideyuki.

In: Developmental Biology, Vol. 176, No. 2, 15.06.1996, p. 230-242.

Research output: Contribution to journalArticle

Sakakibara, S, Imai, T, Hamaguchi, K, Okabe, M, Aruga, J, Nakajima, K, Yasutomi, D, Nagata, T, Kurihara, Y, Uesugi, S, Miyata, T, Ogawa, M, Mikoshiba, K & Okano, H 1996, 'Mouse-Musashi-1, a neural RNA-Binding protein highly enriched in the mammalian CNS stem cell', Developmental Biology, vol. 176, no. 2, pp. 230-242. https://doi.org/10.1006/dbio.1996.0130
Sakakibara, Shinichi ; Imai, Takao ; Hamaguchi, Kayoko ; Okabe, Masataka ; Aruga, Jun ; Nakajima, Kazunori ; Yasutomi, Daisuke ; Nagata, Takashi ; Kurihara, Yasuyuki ; Uesugi, Seiichi ; Miyata, Takaki ; Ogawa, Masaharu ; Mikoshiba, Katsuhiko ; Okano, Hideyuki. / Mouse-Musashi-1, a neural RNA-Binding protein highly enriched in the mammalian CNS stem cell. In: Developmental Biology. 1996 ; Vol. 176, No. 2. pp. 230-242.
@article{d19d6c03822445ef83638f829b01c8eb,
title = "Mouse-Musashi-1, a neural RNA-Binding protein highly enriched in the mammalian CNS stem cell",
abstract = "There is increasing interest in the role of RNA-binding proteins during neural development. Drosophila Musashi is one of the neural RNA-binding proteins essential for neural development and required for asymmetric cell divisions in the Drosophila adult sensory organ development. Here, a novel mammalian neural RNA-binding protein, mouse Musashi-1, was identified based on the homology to Drosophila Musashi and Xenopus NRP-1. In the developing CNS, mouse-Musashi-1 protein was highly enriched in the CNS stem cell. Single-cell culture experiments indicated that mouse Musashi-1 expression is associated with neural precursor cells that are capable of generating neurons and glia. In contrast, in fully differentiated neuronal and glial cells mouse-Musashi-1 expression is lost. This expression pattern of mouse-Musashi-1 is complementary to that of another mammalian neural RNA-binding protein, Hu (a mammalian homologue of a Drosophila neuronal RNA-binding protein Elav), that is expressed in postmitotic neurons within the CNS. In vitro studies indicated that mouse-Musashi-1 possesses binding preferences on poly(G) RNA homopolymer, whereas Hu is known to preferentially bind to short A/U-rich regions in RNA. Based on their differential expression patterns and distinct preferential target RNA sequences, we believe that the mouse-Musashi-1 and Hu proteins may play distinct roles in neurogenesis, either through sequential regulatory mechanisms or differential sorting of mRNA populations during asymmetric division of neural precursor cells.",
author = "Shinichi Sakakibara and Takao Imai and Kayoko Hamaguchi and Masataka Okabe and Jun Aruga and Kazunori Nakajima and Daisuke Yasutomi and Takashi Nagata and Yasuyuki Kurihara and Seiichi Uesugi and Takaki Miyata and Masaharu Ogawa and Katsuhiko Mikoshiba and Hideyuki Okano",
year = "1996",
month = "6",
day = "15",
doi = "10.1006/dbio.1996.0130",
language = "English",
volume = "176",
pages = "230--242",
journal = "Developmental Biology",
issn = "0012-1606",
publisher = "Academic Press Inc.",
number = "2",

}

TY - JOUR

T1 - Mouse-Musashi-1, a neural RNA-Binding protein highly enriched in the mammalian CNS stem cell

AU - Sakakibara, Shinichi

AU - Imai, Takao

AU - Hamaguchi, Kayoko

AU - Okabe, Masataka

AU - Aruga, Jun

AU - Nakajima, Kazunori

AU - Yasutomi, Daisuke

AU - Nagata, Takashi

AU - Kurihara, Yasuyuki

AU - Uesugi, Seiichi

AU - Miyata, Takaki

AU - Ogawa, Masaharu

AU - Mikoshiba, Katsuhiko

AU - Okano, Hideyuki

PY - 1996/6/15

Y1 - 1996/6/15

N2 - There is increasing interest in the role of RNA-binding proteins during neural development. Drosophila Musashi is one of the neural RNA-binding proteins essential for neural development and required for asymmetric cell divisions in the Drosophila adult sensory organ development. Here, a novel mammalian neural RNA-binding protein, mouse Musashi-1, was identified based on the homology to Drosophila Musashi and Xenopus NRP-1. In the developing CNS, mouse-Musashi-1 protein was highly enriched in the CNS stem cell. Single-cell culture experiments indicated that mouse Musashi-1 expression is associated with neural precursor cells that are capable of generating neurons and glia. In contrast, in fully differentiated neuronal and glial cells mouse-Musashi-1 expression is lost. This expression pattern of mouse-Musashi-1 is complementary to that of another mammalian neural RNA-binding protein, Hu (a mammalian homologue of a Drosophila neuronal RNA-binding protein Elav), that is expressed in postmitotic neurons within the CNS. In vitro studies indicated that mouse-Musashi-1 possesses binding preferences on poly(G) RNA homopolymer, whereas Hu is known to preferentially bind to short A/U-rich regions in RNA. Based on their differential expression patterns and distinct preferential target RNA sequences, we believe that the mouse-Musashi-1 and Hu proteins may play distinct roles in neurogenesis, either through sequential regulatory mechanisms or differential sorting of mRNA populations during asymmetric division of neural precursor cells.

AB - There is increasing interest in the role of RNA-binding proteins during neural development. Drosophila Musashi is one of the neural RNA-binding proteins essential for neural development and required for asymmetric cell divisions in the Drosophila adult sensory organ development. Here, a novel mammalian neural RNA-binding protein, mouse Musashi-1, was identified based on the homology to Drosophila Musashi and Xenopus NRP-1. In the developing CNS, mouse-Musashi-1 protein was highly enriched in the CNS stem cell. Single-cell culture experiments indicated that mouse Musashi-1 expression is associated with neural precursor cells that are capable of generating neurons and glia. In contrast, in fully differentiated neuronal and glial cells mouse-Musashi-1 expression is lost. This expression pattern of mouse-Musashi-1 is complementary to that of another mammalian neural RNA-binding protein, Hu (a mammalian homologue of a Drosophila neuronal RNA-binding protein Elav), that is expressed in postmitotic neurons within the CNS. In vitro studies indicated that mouse-Musashi-1 possesses binding preferences on poly(G) RNA homopolymer, whereas Hu is known to preferentially bind to short A/U-rich regions in RNA. Based on their differential expression patterns and distinct preferential target RNA sequences, we believe that the mouse-Musashi-1 and Hu proteins may play distinct roles in neurogenesis, either through sequential regulatory mechanisms or differential sorting of mRNA populations during asymmetric division of neural precursor cells.

UR - http://www.scopus.com/inward/record.url?scp=20244365304&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=20244365304&partnerID=8YFLogxK

U2 - 10.1006/dbio.1996.0130

DO - 10.1006/dbio.1996.0130

M3 - Article

VL - 176

SP - 230

EP - 242

JO - Developmental Biology

JF - Developmental Biology

SN - 0012-1606

IS - 2

ER -