Mouse testis transcriptome reveled using serial analysis of gene expression

Junjie Yao, Takuya Chiba, Jun Sakai, Kunitaka Hirose, Mikio Yamamoto, Akiyuki Hada, Kazunao Kuramoto, Keiichi Higuchi, Masayuki Mori

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

We applied serial analysis of gene expression (SAGE) to the mouse testis to reveal the global gene expression profile and to identify senescence-dependent changes in that profile. A total of 61,929 SAGE tags, including 19,323 unique tags, were obtained from 3- and 29-month-old BDF1 mice and 14-month-old SAMP1 mice. Genes highly expressed in the testis included those associated with spermatogenesis, protein metabolism, energy metabolism, growth and differentiation, and signal transduction. Testes from old mice of both strains appeared atrophied. Morphological examination of aged testes revealed extremely thin seminiferous epithelia and significantly decreased numbers of spermatids and spermatocytes. Despite the physical deterioration, no gross changes in the gene expression profile were apparent in the testes of old BDF1 mice. However, in 14-month-old SAMP1 mice, protamine 2 gene transcription was approximately 50% lower than in BDF1 mice. This reduction may be associated with the oligozoospermia and early decline in reproductive performance of SAMP1 mice. Our SAGE results are the first quantitative gene expression profile of the mouse testis and provide a reliable transcriptome reference for this organ.

Original languageEnglish
Pages (from-to)433-451
Number of pages19
JournalMammalian Genome
Volume15
Issue number6
DOIs
Publication statusPublished - 2004 Jun
Externally publishedYes

Fingerprint

Transcriptome
Testis
Gene Expression
Seminiferous Epithelium
Oligospermia
Spermatocytes
Spermatids
Spermatogenesis
Energy Metabolism
Genes
Signal Transduction
Growth
Proteins

ASJC Scopus subject areas

  • Genetics

Cite this

Yao, J., Chiba, T., Sakai, J., Hirose, K., Yamamoto, M., Hada, A., ... Mori, M. (2004). Mouse testis transcriptome reveled using serial analysis of gene expression. Mammalian Genome, 15(6), 433-451. https://doi.org/10.1007/s00335-004-2347-7

Mouse testis transcriptome reveled using serial analysis of gene expression. / Yao, Junjie; Chiba, Takuya; Sakai, Jun; Hirose, Kunitaka; Yamamoto, Mikio; Hada, Akiyuki; Kuramoto, Kazunao; Higuchi, Keiichi; Mori, Masayuki.

In: Mammalian Genome, Vol. 15, No. 6, 06.2004, p. 433-451.

Research output: Contribution to journalArticle

Yao, J, Chiba, T, Sakai, J, Hirose, K, Yamamoto, M, Hada, A, Kuramoto, K, Higuchi, K & Mori, M 2004, 'Mouse testis transcriptome reveled using serial analysis of gene expression', Mammalian Genome, vol. 15, no. 6, pp. 433-451. https://doi.org/10.1007/s00335-004-2347-7
Yao, Junjie ; Chiba, Takuya ; Sakai, Jun ; Hirose, Kunitaka ; Yamamoto, Mikio ; Hada, Akiyuki ; Kuramoto, Kazunao ; Higuchi, Keiichi ; Mori, Masayuki. / Mouse testis transcriptome reveled using serial analysis of gene expression. In: Mammalian Genome. 2004 ; Vol. 15, No. 6. pp. 433-451.
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