Movement of endoplasmic reticulum in the living axon is distinct from other membranous vesicles in its rate, form, and sensitivity to microtubule inhibitors

Yuko Aihara, Takafumi Inoue, Tomoko Tashiro, Koichi Okamoto, Yoshiaki Komiya, Katsuhiko Mikoshiba

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

The endoplasmic reticulum (ER) is the major membranous component present throughout the axon. Although other membranous structures such as synaptic vesicles are known to move via fast axonal transport, the dynamics of ER in the axon still remains unknown. To study the dynamics of ER in the axon, we have directly visualized the movement of two ER-specific membrane proteins, the sarcoplasmic/endoplasmic reticulum calcium-ATPase and the inositol 1,4,5-trisphosphate receptor, both of which were tagged with green fluorescence protein (GFP) and expressed in cultured chick dorsal root ganglion neurons. In contrast to GFP-tagged synaptophysin that moved as vesicles at 1 μm/sec predominantly in the anterograde direction in the typical style of fast axonal transport, the two ER proteins did not move in a discrete vesicular form. Their movement determined by the fluorescence recovery after photobleaching technique was bi-directional, 10-fold slower (∼0.1 μm/sec), and temperature-sensitive. The rate of movement of ER was also sensitive to Iow doses of vinblastine and nocodazole that did not affect the rate of synaptophysin-GFP, further suggesting that it is also distinct from the well-documented movement of membranous vesicles in its relation with microtubules.

Original languageEnglish
Pages (from-to)236-246
Number of pages11
JournalJournal of Neuroscience Research
Volume65
Issue number3
DOIs
Publication statusPublished - 2001 Aug 1
Externally publishedYes

Fingerprint

Microtubules
Endoplasmic Reticulum
Axons
Synaptophysin
Axonal Transport
Fluorescence
Proteins
Sarcoplasmic Reticulum Calcium-Transporting ATPases
Fluorescence Recovery After Photobleaching
Nocodazole
Vinblastine
Synaptic Vesicles
Spinal Ganglia
Inositol
Membrane Proteins
Neurons
Temperature

Keywords

  • ER
  • FRAP
  • Inositol 1,4,5-trisphosphate receptor
  • Microtubule inhibitors
  • Sarcoplasmic/endoplasmic reticulum calcium ATPase

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Movement of endoplasmic reticulum in the living axon is distinct from other membranous vesicles in its rate, form, and sensitivity to microtubule inhibitors. / Aihara, Yuko; Inoue, Takafumi; Tashiro, Tomoko; Okamoto, Koichi; Komiya, Yoshiaki; Mikoshiba, Katsuhiko.

In: Journal of Neuroscience Research, Vol. 65, No. 3, 01.08.2001, p. 236-246.

Research output: Contribution to journalArticle

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