Multi-reagents high speed exchange flow system for single biomolecutar dynamics real time monitoring

Yukiko Sato, Takahiro Arakawa, Tomoya Sameshima, Taro Ueno, Takashi Funatsu, Shuichi Shoji

    Research output: Chapter in Book/Report/Conference proceedingConference contribution

    Abstract

    We develop a multi-reagent high speed exchange flow system integrated with Total Internal Reflection Fluorescent Microscopy (TIRFM). The reagent switching time within 100 ms was obtained with high performance in-channel PDMS microvalves, the real time monitoring of a single protein behavior, the chaperonin GroEL - cochaperonin GroES rapid interaction, was performed. The fabricated TIRFM microflow system enables the observation of single biomolecular level very short time reactions of a few seconds.

    Original languageEnglish
    Title of host publicationMicro Total Analysis Systems - Proceedings of MicroTAS 2006 Conference: 10th International Conference on Miniaturized Systems for Chemistry and Life Sciences
    PublisherJapan Academic Association Inc
    Pages603-605
    Number of pages3
    Publication statusPublished - 2006
    Event10th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2006 - Tokyo
    Duration: 2006 Nov 52006 Nov 9

    Other

    Other10th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2006
    CityTokyo
    Period06/11/506/11/9

    Fingerprint

    Microscopic examination
    Chaperonins
    Monitoring
    Proteins

    Keywords

    • Microflow system
    • PDMS microvalves
    • Protein analysis
    • Real time monitoring
    • Single biomolecular detection

    ASJC Scopus subject areas

    • Chemical Engineering (miscellaneous)
    • Bioengineering

    Cite this

    Sato, Y., Arakawa, T., Sameshima, T., Ueno, T., Funatsu, T., & Shoji, S. (2006). Multi-reagents high speed exchange flow system for single biomolecutar dynamics real time monitoring. In Micro Total Analysis Systems - Proceedings of MicroTAS 2006 Conference: 10th International Conference on Miniaturized Systems for Chemistry and Life Sciences (pp. 603-605). Japan Academic Association Inc.

    Multi-reagents high speed exchange flow system for single biomolecutar dynamics real time monitoring. / Sato, Yukiko; Arakawa, Takahiro; Sameshima, Tomoya; Ueno, Taro; Funatsu, Takashi; Shoji, Shuichi.

    Micro Total Analysis Systems - Proceedings of MicroTAS 2006 Conference: 10th International Conference on Miniaturized Systems for Chemistry and Life Sciences. Japan Academic Association Inc, 2006. p. 603-605.

    Research output: Chapter in Book/Report/Conference proceedingConference contribution

    Sato, Y, Arakawa, T, Sameshima, T, Ueno, T, Funatsu, T & Shoji, S 2006, Multi-reagents high speed exchange flow system for single biomolecutar dynamics real time monitoring. in Micro Total Analysis Systems - Proceedings of MicroTAS 2006 Conference: 10th International Conference on Miniaturized Systems for Chemistry and Life Sciences. Japan Academic Association Inc, pp. 603-605, 10th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2006, Tokyo, 06/11/5.
    Sato Y, Arakawa T, Sameshima T, Ueno T, Funatsu T, Shoji S. Multi-reagents high speed exchange flow system for single biomolecutar dynamics real time monitoring. In Micro Total Analysis Systems - Proceedings of MicroTAS 2006 Conference: 10th International Conference on Miniaturized Systems for Chemistry and Life Sciences. Japan Academic Association Inc. 2006. p. 603-605
    Sato, Yukiko ; Arakawa, Takahiro ; Sameshima, Tomoya ; Ueno, Taro ; Funatsu, Takashi ; Shoji, Shuichi. / Multi-reagents high speed exchange flow system for single biomolecutar dynamics real time monitoring. Micro Total Analysis Systems - Proceedings of MicroTAS 2006 Conference: 10th International Conference on Miniaturized Systems for Chemistry and Life Sciences. Japan Academic Association Inc, 2006. pp. 603-605
    @inproceedings{9e95ea59ee2d4dd794e826e636aa75e5,
    title = "Multi-reagents high speed exchange flow system for single biomolecutar dynamics real time monitoring",
    abstract = "We develop a multi-reagent high speed exchange flow system integrated with Total Internal Reflection Fluorescent Microscopy (TIRFM). The reagent switching time within 100 ms was obtained with high performance in-channel PDMS microvalves, the real time monitoring of a single protein behavior, the chaperonin GroEL - cochaperonin GroES rapid interaction, was performed. The fabricated TIRFM microflow system enables the observation of single biomolecular level very short time reactions of a few seconds.",
    keywords = "Microflow system, PDMS microvalves, Protein analysis, Real time monitoring, Single biomolecular detection",
    author = "Yukiko Sato and Takahiro Arakawa and Tomoya Sameshima and Taro Ueno and Takashi Funatsu and Shuichi Shoji",
    year = "2006",
    language = "English",
    pages = "603--605",
    booktitle = "Micro Total Analysis Systems - Proceedings of MicroTAS 2006 Conference: 10th International Conference on Miniaturized Systems for Chemistry and Life Sciences",
    publisher = "Japan Academic Association Inc",

    }

    TY - GEN

    T1 - Multi-reagents high speed exchange flow system for single biomolecutar dynamics real time monitoring

    AU - Sato, Yukiko

    AU - Arakawa, Takahiro

    AU - Sameshima, Tomoya

    AU - Ueno, Taro

    AU - Funatsu, Takashi

    AU - Shoji, Shuichi

    PY - 2006

    Y1 - 2006

    N2 - We develop a multi-reagent high speed exchange flow system integrated with Total Internal Reflection Fluorescent Microscopy (TIRFM). The reagent switching time within 100 ms was obtained with high performance in-channel PDMS microvalves, the real time monitoring of a single protein behavior, the chaperonin GroEL - cochaperonin GroES rapid interaction, was performed. The fabricated TIRFM microflow system enables the observation of single biomolecular level very short time reactions of a few seconds.

    AB - We develop a multi-reagent high speed exchange flow system integrated with Total Internal Reflection Fluorescent Microscopy (TIRFM). The reagent switching time within 100 ms was obtained with high performance in-channel PDMS microvalves, the real time monitoring of a single protein behavior, the chaperonin GroEL - cochaperonin GroES rapid interaction, was performed. The fabricated TIRFM microflow system enables the observation of single biomolecular level very short time reactions of a few seconds.

    KW - Microflow system

    KW - PDMS microvalves

    KW - Protein analysis

    KW - Real time monitoring

    KW - Single biomolecular detection

    UR - http://www.scopus.com/inward/record.url?scp=84902436762&partnerID=8YFLogxK

    UR - http://www.scopus.com/inward/citedby.url?scp=84902436762&partnerID=8YFLogxK

    M3 - Conference contribution

    SP - 603

    EP - 605

    BT - Micro Total Analysis Systems - Proceedings of MicroTAS 2006 Conference: 10th International Conference on Miniaturized Systems for Chemistry and Life Sciences

    PB - Japan Academic Association Inc

    ER -