Multi-reagents high speed exchange flow system for single biomolecutar dynamics real time monitoring

Yukiko Sato, Takahiro Arakawa, Tomoya Sameshima, Taro Ueno, Takashi Funatsu, Shuichi Shoji

Research output: Contribution to conferencePaperpeer-review

Abstract

We develop a multi-reagent high speed exchange flow system integrated with Total Internal Reflection Fluorescent Microscopy (TIRFM). The reagent switching time within 100 ms was obtained with high performance in-channel PDMS microvalves, the real time monitoring of a single protein behavior, the chaperonin GroEL - cochaperonin GroES rapid interaction, was performed. The fabricated TIRFM microflow system enables the observation of single biomolecular level very short time reactions of a few seconds.

Original languageEnglish
Pages603-605
Number of pages3
Publication statusPublished - 2006 Jan 1
Event10th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2006 - Tokyo, Japan
Duration: 2006 Nov 52006 Nov 9

Conference

Conference10th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2006
CountryJapan
CityTokyo
Period06/11/506/11/9

Keywords

  • Microflow system
  • PDMS microvalves
  • Protein analysis
  • Real time monitoring
  • Single biomolecular detection

ASJC Scopus subject areas

  • Chemical Engineering (miscellaneous)
  • Bioengineering

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