Multi-reagents high speed exchange flow system for single biomolecutar dynamics real time monitoring

Yukiko Sato, Takahiro Arakawa, Tomoya Sameshima, Taro Ueno, Takashi Funatsu, Shuichi Shoji

    Research output: Chapter in Book/Report/Conference proceedingConference contribution

    Abstract

    We develop a multi-reagent high speed exchange flow system integrated with Total Internal Reflection Fluorescent Microscopy (TIRFM). The reagent switching time within 100 ms was obtained with high performance in-channel PDMS microvalves, the real time monitoring of a single protein behavior, the chaperonin GroEL - cochaperonin GroES rapid interaction, was performed. The fabricated TIRFM microflow system enables the observation of single biomolecular level very short time reactions of a few seconds.

    Original languageEnglish
    Title of host publicationMicro Total Analysis Systems - Proceedings of MicroTAS 2006 Conference: 10th International Conference on Miniaturized Systems for Chemistry and Life Sciences
    PublisherJapan Academic Association Inc
    Pages603-605
    Number of pages3
    Publication statusPublished - 2006
    Event10th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2006 - Tokyo
    Duration: 2006 Nov 52006 Nov 9

    Other

    Other10th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2006
    CityTokyo
    Period06/11/506/11/9

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    Keywords

    • Microflow system
    • PDMS microvalves
    • Protein analysis
    • Real time monitoring
    • Single biomolecular detection

    ASJC Scopus subject areas

    • Chemical Engineering (miscellaneous)
    • Bioengineering

    Cite this

    Sato, Y., Arakawa, T., Sameshima, T., Ueno, T., Funatsu, T., & Shoji, S. (2006). Multi-reagents high speed exchange flow system for single biomolecutar dynamics real time monitoring. In Micro Total Analysis Systems - Proceedings of MicroTAS 2006 Conference: 10th International Conference on Miniaturized Systems for Chemistry and Life Sciences (pp. 603-605). Japan Academic Association Inc.