Multifactorial regulation of daily rhythms in expression of the metabolically responsive gene Spot14 in the mouse liver

Akinori Ishihara, Eriko Matsumoto, Kazumasa Horikawa, Takashi Kudo, Eiko Sakao, Ayako Nemoto, Katsuro Iwase, Hajime Sugiyama, Yutaka Tamura, Shigenobu Shibata, Masaki Takiguchi

    Research output: Contribution to journalArticle

    8 Citations (Scopus)

    Abstract

    Spot14 is a putative transcriptional regulator for genes involved in fatty acid synthesis. The Spot14 gene is activated in response to lipogenic stimuli such as dietary carbohydrate and is also under circadian regulation. The authors investigated factors responsible for daily oscillation of Spot14 expression. If mice were kept under a 12-h light/12-h dark cycle with ad libitum feeding, Spot14 mRNA levels in the liver reached a peak at an early dark period when mice, as nocturnal animals, start feeding. Under fasting, while Spot14 mRNA levels were generally decreased, the rhythmicity was still maintained, suggesting contribution of both nutritional elements and circadian clock factors on robust rhythmicity of Spot14 expression. Effects of circadian clock factors were confirmed by the observations that the circadian rhythm of Spot14 expression was seen also under the constant darkness and that the rhythmicity was lost in Clock mutant mice. When mice were housed in short-photoperiod (6-h light/18-h dark) and long-photoperiod (18-h light/6-h dark) cycles, rhythms of Spot14 mRNA levels were phase advanced and phase delayed, respectively, being concordant with the notion that Spot14 expression is under the control of the light-entrainable oscillator. As for nutritional mediators, in the liver of db/db mice exhibiting hyperinsulinemia-accompanied hyperglycemia, Spot14 mRNA levels were constantly high without apparent rhythmicity, consistent with previous observations for strong activation of the Spot14 gene by glucose and insulin. Restricted feeding during the 4-h mid-light period caused a phase advance of the Spot14 expression rhythm. On the other hand, restricted feeding during the 4-h mid-dark period led to damping of the rhythmicity, apparently resulting from the separation of phases between effects of the light/dark cycle and feeding on Spot14 expression. Thus, the daily rhythm of Spot14 expression in the liver is under the control of the light-entrainable oscillator, food-entrainable oscillator, and food-derived nutrients, in a separate or cooperative manner.

    Original languageEnglish
    Pages (from-to)324-334
    Number of pages11
    JournalJournal of Biological Rhythms
    Volume22
    Issue number4
    DOIs
    Publication statusPublished - 2007 Aug

    Fingerprint

    Periodicity
    Light
    liver
    Liver
    circadian rhythm
    mice
    Photoperiod
    photoperiod
    Genes
    restricted feeding
    scotophase
    Messenger RNA
    Circadian Clocks
    genes
    Food
    ad libitum feeding
    gene activation
    dietary carbohydrate
    hyperinsulinemia
    Dietary Carbohydrates

    Keywords

    • Clock genes
    • Clock output genes
    • Food-entrainable oscillator
    • Light-entrainable oscillator
    • Lipid metabolism
    • Restricted feeding

    ASJC Scopus subject areas

    • Agricultural and Biological Sciences (miscellaneous)
    • Physiology
    • Physiology (medical)

    Cite this

    Multifactorial regulation of daily rhythms in expression of the metabolically responsive gene Spot14 in the mouse liver. / Ishihara, Akinori; Matsumoto, Eriko; Horikawa, Kazumasa; Kudo, Takashi; Sakao, Eiko; Nemoto, Ayako; Iwase, Katsuro; Sugiyama, Hajime; Tamura, Yutaka; Shibata, Shigenobu; Takiguchi, Masaki.

    In: Journal of Biological Rhythms, Vol. 22, No. 4, 08.2007, p. 324-334.

    Research output: Contribution to journalArticle

    Ishihara, A, Matsumoto, E, Horikawa, K, Kudo, T, Sakao, E, Nemoto, A, Iwase, K, Sugiyama, H, Tamura, Y, Shibata, S & Takiguchi, M 2007, 'Multifactorial regulation of daily rhythms in expression of the metabolically responsive gene Spot14 in the mouse liver', Journal of Biological Rhythms, vol. 22, no. 4, pp. 324-334. https://doi.org/10.1177/0748730407302107
    Ishihara, Akinori ; Matsumoto, Eriko ; Horikawa, Kazumasa ; Kudo, Takashi ; Sakao, Eiko ; Nemoto, Ayako ; Iwase, Katsuro ; Sugiyama, Hajime ; Tamura, Yutaka ; Shibata, Shigenobu ; Takiguchi, Masaki. / Multifactorial regulation of daily rhythms in expression of the metabolically responsive gene Spot14 in the mouse liver. In: Journal of Biological Rhythms. 2007 ; Vol. 22, No. 4. pp. 324-334.
    @article{b64e7f365c6141d88b49b27673e4529e,
    title = "Multifactorial regulation of daily rhythms in expression of the metabolically responsive gene Spot14 in the mouse liver",
    abstract = "Spot14 is a putative transcriptional regulator for genes involved in fatty acid synthesis. The Spot14 gene is activated in response to lipogenic stimuli such as dietary carbohydrate and is also under circadian regulation. The authors investigated factors responsible for daily oscillation of Spot14 expression. If mice were kept under a 12-h light/12-h dark cycle with ad libitum feeding, Spot14 mRNA levels in the liver reached a peak at an early dark period when mice, as nocturnal animals, start feeding. Under fasting, while Spot14 mRNA levels were generally decreased, the rhythmicity was still maintained, suggesting contribution of both nutritional elements and circadian clock factors on robust rhythmicity of Spot14 expression. Effects of circadian clock factors were confirmed by the observations that the circadian rhythm of Spot14 expression was seen also under the constant darkness and that the rhythmicity was lost in Clock mutant mice. When mice were housed in short-photoperiod (6-h light/18-h dark) and long-photoperiod (18-h light/6-h dark) cycles, rhythms of Spot14 mRNA levels were phase advanced and phase delayed, respectively, being concordant with the notion that Spot14 expression is under the control of the light-entrainable oscillator. As for nutritional mediators, in the liver of db/db mice exhibiting hyperinsulinemia-accompanied hyperglycemia, Spot14 mRNA levels were constantly high without apparent rhythmicity, consistent with previous observations for strong activation of the Spot14 gene by glucose and insulin. Restricted feeding during the 4-h mid-light period caused a phase advance of the Spot14 expression rhythm. On the other hand, restricted feeding during the 4-h mid-dark period led to damping of the rhythmicity, apparently resulting from the separation of phases between effects of the light/dark cycle and feeding on Spot14 expression. Thus, the daily rhythm of Spot14 expression in the liver is under the control of the light-entrainable oscillator, food-entrainable oscillator, and food-derived nutrients, in a separate or cooperative manner.",
    keywords = "Clock genes, Clock output genes, Food-entrainable oscillator, Light-entrainable oscillator, Lipid metabolism, Restricted feeding",
    author = "Akinori Ishihara and Eriko Matsumoto and Kazumasa Horikawa and Takashi Kudo and Eiko Sakao and Ayako Nemoto and Katsuro Iwase and Hajime Sugiyama and Yutaka Tamura and Shigenobu Shibata and Masaki Takiguchi",
    year = "2007",
    month = "8",
    doi = "10.1177/0748730407302107",
    language = "English",
    volume = "22",
    pages = "324--334",
    journal = "Journal of Biological Rhythms",
    issn = "0748-7304",
    publisher = "SAGE Publications Inc.",
    number = "4",

    }

    TY - JOUR

    T1 - Multifactorial regulation of daily rhythms in expression of the metabolically responsive gene Spot14 in the mouse liver

    AU - Ishihara, Akinori

    AU - Matsumoto, Eriko

    AU - Horikawa, Kazumasa

    AU - Kudo, Takashi

    AU - Sakao, Eiko

    AU - Nemoto, Ayako

    AU - Iwase, Katsuro

    AU - Sugiyama, Hajime

    AU - Tamura, Yutaka

    AU - Shibata, Shigenobu

    AU - Takiguchi, Masaki

    PY - 2007/8

    Y1 - 2007/8

    N2 - Spot14 is a putative transcriptional regulator for genes involved in fatty acid synthesis. The Spot14 gene is activated in response to lipogenic stimuli such as dietary carbohydrate and is also under circadian regulation. The authors investigated factors responsible for daily oscillation of Spot14 expression. If mice were kept under a 12-h light/12-h dark cycle with ad libitum feeding, Spot14 mRNA levels in the liver reached a peak at an early dark period when mice, as nocturnal animals, start feeding. Under fasting, while Spot14 mRNA levels were generally decreased, the rhythmicity was still maintained, suggesting contribution of both nutritional elements and circadian clock factors on robust rhythmicity of Spot14 expression. Effects of circadian clock factors were confirmed by the observations that the circadian rhythm of Spot14 expression was seen also under the constant darkness and that the rhythmicity was lost in Clock mutant mice. When mice were housed in short-photoperiod (6-h light/18-h dark) and long-photoperiod (18-h light/6-h dark) cycles, rhythms of Spot14 mRNA levels were phase advanced and phase delayed, respectively, being concordant with the notion that Spot14 expression is under the control of the light-entrainable oscillator. As for nutritional mediators, in the liver of db/db mice exhibiting hyperinsulinemia-accompanied hyperglycemia, Spot14 mRNA levels were constantly high without apparent rhythmicity, consistent with previous observations for strong activation of the Spot14 gene by glucose and insulin. Restricted feeding during the 4-h mid-light period caused a phase advance of the Spot14 expression rhythm. On the other hand, restricted feeding during the 4-h mid-dark period led to damping of the rhythmicity, apparently resulting from the separation of phases between effects of the light/dark cycle and feeding on Spot14 expression. Thus, the daily rhythm of Spot14 expression in the liver is under the control of the light-entrainable oscillator, food-entrainable oscillator, and food-derived nutrients, in a separate or cooperative manner.

    AB - Spot14 is a putative transcriptional regulator for genes involved in fatty acid synthesis. The Spot14 gene is activated in response to lipogenic stimuli such as dietary carbohydrate and is also under circadian regulation. The authors investigated factors responsible for daily oscillation of Spot14 expression. If mice were kept under a 12-h light/12-h dark cycle with ad libitum feeding, Spot14 mRNA levels in the liver reached a peak at an early dark period when mice, as nocturnal animals, start feeding. Under fasting, while Spot14 mRNA levels were generally decreased, the rhythmicity was still maintained, suggesting contribution of both nutritional elements and circadian clock factors on robust rhythmicity of Spot14 expression. Effects of circadian clock factors were confirmed by the observations that the circadian rhythm of Spot14 expression was seen also under the constant darkness and that the rhythmicity was lost in Clock mutant mice. When mice were housed in short-photoperiod (6-h light/18-h dark) and long-photoperiod (18-h light/6-h dark) cycles, rhythms of Spot14 mRNA levels were phase advanced and phase delayed, respectively, being concordant with the notion that Spot14 expression is under the control of the light-entrainable oscillator. As for nutritional mediators, in the liver of db/db mice exhibiting hyperinsulinemia-accompanied hyperglycemia, Spot14 mRNA levels were constantly high without apparent rhythmicity, consistent with previous observations for strong activation of the Spot14 gene by glucose and insulin. Restricted feeding during the 4-h mid-light period caused a phase advance of the Spot14 expression rhythm. On the other hand, restricted feeding during the 4-h mid-dark period led to damping of the rhythmicity, apparently resulting from the separation of phases between effects of the light/dark cycle and feeding on Spot14 expression. Thus, the daily rhythm of Spot14 expression in the liver is under the control of the light-entrainable oscillator, food-entrainable oscillator, and food-derived nutrients, in a separate or cooperative manner.

    KW - Clock genes

    KW - Clock output genes

    KW - Food-entrainable oscillator

    KW - Light-entrainable oscillator

    KW - Lipid metabolism

    KW - Restricted feeding

    UR - http://www.scopus.com/inward/record.url?scp=34447573682&partnerID=8YFLogxK

    UR - http://www.scopus.com/inward/citedby.url?scp=34447573682&partnerID=8YFLogxK

    U2 - 10.1177/0748730407302107

    DO - 10.1177/0748730407302107

    M3 - Article

    C2 - 17660449

    AN - SCOPUS:34447573682

    VL - 22

    SP - 324

    EP - 334

    JO - Journal of Biological Rhythms

    JF - Journal of Biological Rhythms

    SN - 0748-7304

    IS - 4

    ER -