Multiple reporter gene assays for the assessment and estimation of chemical toxicity.

Junko Takahashi, Hitoshi Iwahashi

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)

Abstract

To detect chemical toxicity, we are making new bioassay systems that use promoters selected from yeast DNA microarray experiments. We performed multiple reporter gene assays using the promoters of these genes; the promoter regions were inserted upstream of green fluorescence protein (GFP). In this report, six genes (HSP26, MET17, YLL057C, FIT2, CUP1 and OYE3) were selected and assays were carried out for 55 chemicals. The promoters of these genes showed different responses to chemicals within 4 h. This result indicates that this technique enables us to predict the toxicity of chemicals in the environment and to understand toxicities of newly synthesized chemicals.

Original languageEnglish
Pages (from-to)269-282
Number of pages14
JournalEnvironmental sciences : an international journal of environmental physiology and toxicology
Volume11
Issue number5
Publication statusPublished - 2004
Externally publishedYes

ASJC Scopus subject areas

  • Medicine(all)

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