Multiple-site optical recording for characterization of functional synaptic organization of the optic tectum of rainbow trout

Masae Kinoshita, Risa Ueda, Satoshi Kojima, Katsushige Sato, Masahiko Watanabe, Akihisa Urano, Etsuro Ito

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

To map the functional synaptic organization over a wide area in the optic tectum, we directly monitored two-dimensional propagation of postsynaptic depolarization evoked by firing of retinotectal afferents in optic tectum slices prepared from rainbow trout (Oncorhynchus mykiss), using a voltage-sensitive dye and a photodiode array system. The postsynaptic responses to afferent stimulation first propagated in the stratum opticum and stratum fibrosum et griseum superficiale in an anterograde fashion in the afferents and then expanded vertically into the deep layers. This vertical propagation appeared to occur along a bundle-like structure that corresponded well with a cluster of neurons whose somata are located in the stratum periventriculare. Pharmacological studies showed that these postsynaptic responses were mediated by ionotropic glutamate receptors. On the other hand, the optical signals appeared to consist of at least two components (a transient signal and a slow signal). The second transient signal summated with the first slow signal by paired stimulation, suggesting that the transient and slow signals originated from different cell types. Taken together, these results showed that the functional synaptic organization of the teleost optic tectum comprises of two depolarization-signal propagating paths along a horizontal layer structure and a vertical bundle-like structure and that these synaptic responses occur via glutamatergic transmission.

Original languageEnglish
Pages (from-to)868-876
Number of pages9
JournalEuropean Journal of Neuroscience
Volume16
Issue number5
DOIs
Publication statusPublished - 2002
Externally publishedYes

Fingerprint

Superior Colliculi
Oncorhynchus mykiss
Ionotropic Glutamate Receptors
Carisoprodol
Coloring Agents
Pharmacology
Neurons

Keywords

  • Glutamate receptor
  • Golgi staining
  • Oncorhynchus mykiss
  • Retinotectal afferent

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Multiple-site optical recording for characterization of functional synaptic organization of the optic tectum of rainbow trout. / Kinoshita, Masae; Ueda, Risa; Kojima, Satoshi; Sato, Katsushige; Watanabe, Masahiko; Urano, Akihisa; Ito, Etsuro.

In: European Journal of Neuroscience, Vol. 16, No. 5, 2002, p. 868-876.

Research output: Contribution to journalArticle

Kinoshita, Masae ; Ueda, Risa ; Kojima, Satoshi ; Sato, Katsushige ; Watanabe, Masahiko ; Urano, Akihisa ; Ito, Etsuro. / Multiple-site optical recording for characterization of functional synaptic organization of the optic tectum of rainbow trout. In: European Journal of Neuroscience. 2002 ; Vol. 16, No. 5. pp. 868-876.
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