Murine retroviral vectors expressing the tax1 gene of human T-cell leukemia virus type 1

Akagi Tsuyoshi, Hiroshi Nyunoya, Shimotohno Kunitada

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Murine retroviral vectors which express the tax1 gene of human T-cell leukemia virus type 1 (HTLV-1) have been constructed. A significant increase in virus titer was achieved by inserting a portion of the gag region of Moloney murine leukemia virus. Using these vectors, tax1 was stably introduced into primary human T-cells derived from peripheral blood lymphocytes. Expression of the functional tax1 in infected cells was confirmed by trans-activation of HTLV-1 long terminal repeat-directed transcription. These vectors provide a useful means of investigating the function of tax1 in natural target cells for HTLV-1.

Original languageEnglish
Pages (from-to)255-259
Number of pages5
JournalGene
Volume106
Issue number2
DOIs
Publication statusPublished - 1991 Oct 15
Externally publishedYes

Fingerprint

Deltaretrovirus
Genes
Moloney murine leukemia virus
Terminal Repeat Sequences
Viral Load
Lymphocytes
T-Lymphocytes

Keywords

  • gene transfer
  • human retrovirus
  • immortalization
  • primary human T lymphocytes
  • Recombinant DNA
  • trans-activation

ASJC Scopus subject areas

  • Genetics

Cite this

Murine retroviral vectors expressing the tax1 gene of human T-cell leukemia virus type 1. / Tsuyoshi, Akagi; Nyunoya, Hiroshi; Kunitada, Shimotohno.

In: Gene, Vol. 106, No. 2, 15.10.1991, p. 255-259.

Research output: Contribution to journalArticle

Tsuyoshi, Akagi ; Nyunoya, Hiroshi ; Kunitada, Shimotohno. / Murine retroviral vectors expressing the tax1 gene of human T-cell leukemia virus type 1. In: Gene. 1991 ; Vol. 106, No. 2. pp. 255-259.
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