Muscle soreness-induced reduction in force generation is accompanied by increased nitric oxide content and DNA damage in human skeletal muscle

Zsolt Radak, Jozsef Pucsok, Sandor Mecseki, Tamas Csont, Peter Ferdinandy

Research output: Contribution to journalArticle

95 Citations (Scopus)


We examined the effect of exercise-induced muscle soreness on maximal force generation, tissue nitric oxide (NO) and 8-hydroxydeoxyguanosine (8- OHdG) content in human skeletal muscle. Female volunteers were assigned to control (C) and muscle soreness (MS) groups (n = 6 in each). MS group performed 200 eccentric muscle actions of the rectus femoris to induce muscle soreness. Maximal force generation was measured 24 h before and after exercise in both groups. Needle biopsy samples were assayed for NO content with electron spin resonance spectroscopy after ex vivo spin trapping, and 8- OHdG content were measured with an enzyme-linked immunoassay. Maximal force decreased by 11 ± 5.4% (p < .05) 24 h after exercise in MS group. Muscle soreness increased NO and 8-OHdG contents from their control values of 0.39 ± 0.08 arbitrary units and 0.035 ± 0.004 pmol/μmol DNA to 0.96 ± 0.05 (p < .05) arbitrary units and 0.044 ± 0.005 (p < .05) pmol/μmol DNA, respectively. This is the first demonstration that muscle soreness-induced decrease in maximal force generation is a result of an increase in muscular NO content and associated with enhanced formation of 8-OHdG in human skeletal muscle.

Original languageEnglish
Pages (from-to)1059-1063
Number of pages5
JournalFree Radical Biology and Medicine
Issue number7-8
Publication statusPublished - 1999 Apr 1
Externally publishedYes



  • 8- Hydroxydeoxyguanosine
  • Exercise
  • Free radicals
  • Human muscle
  • Maximal force
  • Muscle soreness
  • Nitric oxide

ASJC Scopus subject areas

  • Medicine(all)
  • Toxicology
  • Clinical Biochemistry

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