Muscle soreness is associated with enhancement of nitrogen oxide and DNA damage in human skeletal muscle

Zsolt Radak, J. Pucsok, S. Mecseki, T. Csont, L. Boros, S. Ferdinandi

Research output: Contribution to journalArticle

Abstract

The mechanisms behind the muscle soreness are still poorly understood. The aim of the present investigation was to study the possible involvement of free radical species in muscle soreness. The presence of free radicals was measured directly by the determination of NO level using electron spin resonance (ESR) and indirectly by the measurement of DNA adduct of 8-hydroxydeoxyguanosine (8-OHdG) by ELISA method from human skeletal muscle. Fourteen physically active university students voluntarily participated in the study, and were divided into control (C) and exercised (E) group. The E group performed eccentric exercises in the gym and the biopsy samples were taken from the rectus femoris muscle one day after the exercising. The level of muscle soreness was evaluated by questionnaire scaled from zero to ten. The score ranged between 6 and 10 indicating significant muscle soreness at the time of biopsy sampling. There was a massive nearly three fold increase (P <0.0032) in NO level in the E group (0.96±0.05 arbitrary unit) compared with C group (0.39±0.08 arbitrary unit). The concentration of 8-OHdG was about 30% larger (P <0.05) in E group than in the muscle of C subjects. The results of the present study suggest that during muscle soreness there is a massive increase in NO production which is associated with the increase of DNA damage in human skeletal muscle.

Original languageEnglish
JournalFASEB Journal
Volume12
Issue number5
Publication statusPublished - 1998 Mar 20
Externally publishedYes

Fingerprint

nitrogen oxides
Myalgia
DNA damage
DNA Damage
Muscle
skeletal muscle
Nitric Oxide
Skeletal Muscle
muscles
DNA
Free Radicals
Biopsy
Muscles
biopsy
DNA Adducts
Quadriceps Muscle
Electron Spin Resonance Spectroscopy
DNA adducts
electron paramagnetic resonance spectroscopy
Enzyme-Linked Immunosorbent Assay

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Cell Biology

Cite this

Radak, Z., Pucsok, J., Mecseki, S., Csont, T., Boros, L., & Ferdinandi, S. (1998). Muscle soreness is associated with enhancement of nitrogen oxide and DNA damage in human skeletal muscle. FASEB Journal, 12(5).

Muscle soreness is associated with enhancement of nitrogen oxide and DNA damage in human skeletal muscle. / Radak, Zsolt; Pucsok, J.; Mecseki, S.; Csont, T.; Boros, L.; Ferdinandi, S.

In: FASEB Journal, Vol. 12, No. 5, 20.03.1998.

Research output: Contribution to journalArticle

Radak, Z, Pucsok, J, Mecseki, S, Csont, T, Boros, L & Ferdinandi, S 1998, 'Muscle soreness is associated with enhancement of nitrogen oxide and DNA damage in human skeletal muscle', FASEB Journal, vol. 12, no. 5.
Radak Z, Pucsok J, Mecseki S, Csont T, Boros L, Ferdinandi S. Muscle soreness is associated with enhancement of nitrogen oxide and DNA damage in human skeletal muscle. FASEB Journal. 1998 Mar 20;12(5).
Radak, Zsolt ; Pucsok, J. ; Mecseki, S. ; Csont, T. ; Boros, L. ; Ferdinandi, S. / Muscle soreness is associated with enhancement of nitrogen oxide and DNA damage in human skeletal muscle. In: FASEB Journal. 1998 ; Vol. 12, No. 5.
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