Mutant mice lacking ryanodine receptor type 3 exhibit deficits of contextual fear conditioning and activation of calcium/calmodulin-dependent protein kinase II in the hippocampus

Yasuko Kouzu, Takahiro Moriya, Hiroshi Takeshima, Tohru Yoshioka, Shigenobu Shibata

    Research output: Contribution to journalArticle

    50 Citations (Scopus)

    Abstract

    As it is known that ryanodine receptor type 3 is expressed in the hippocampus, we examined the contribution of this receptor to contextual fear conditioning behavior and to the activation of Ca2+/calmodulin-dependent protein kinase II using mice lacking the receptor. Ryanodine receptor type 3-deficient mice exhibited impairments of performance in the contextual fear conditioning test, passive avoidance test, and Y-maze learning test. Both the activities of Ca2+/calmodulin-dependent protein kinase IIβ and Ca2+/calmodulin-dependent protein kinase IIα were significantly increased in the experimental group compared to the control group in the hippocampus, but not in the cingulate cortex on the testing day 24 h after contextual fear training. However, the activities of Ca2+/calmodulin-dependent protein kinase IIβ and α were almost the same in the experimental and control groups in the hippocampus on the training day. Ryanodine receptor type 3-deficient mice did not show the increment of Ca2+/calmodulin-dependent protein kinase IIβ and α activities in the hippocampus on the testing day. In addition, these mutant mice showed the reduction of fear response in the elevated plus-maze test. The present results suggest that calcium-induced calcium release through the activation of ryanodine receptor type 3 in the hippocampus is important to the expression of the performance of contextual learning through the elevation of Ca2+/calmodulin-dependent protein kinase IIβ and α activities. Copyright (C) 2000 Elsevier Science B.V.

    Original languageEnglish
    Pages (from-to)142-150
    Number of pages9
    JournalMolecular Brain Research
    Volume76
    Issue number1
    DOIs
    Publication statusPublished - 2000 Mar 10

    Fingerprint

    Calcium-Calmodulin-Dependent Protein Kinase Type 2
    Calcium-Calmodulin-Dependent Protein Kinases
    Ryanodine Receptor Calcium Release Channel
    Fear
    Hippocampus
    Maze Learning
    Calcium
    Control Groups
    Gyrus Cinguli
    Conditioning (Psychology)
    Learning

    Keywords

    • Ca/calmodulin-dependent protein kinase II
    • Fear conditioning
    • Hippocampus
    • Knockout mouse
    • Memory
    • Ryanodine receptor

    ASJC Scopus subject areas

    • Molecular Biology
    • Cellular and Molecular Neuroscience

    Cite this

    Mutant mice lacking ryanodine receptor type 3 exhibit deficits of contextual fear conditioning and activation of calcium/calmodulin-dependent protein kinase II in the hippocampus. / Kouzu, Yasuko; Moriya, Takahiro; Takeshima, Hiroshi; Yoshioka, Tohru; Shibata, Shigenobu.

    In: Molecular Brain Research, Vol. 76, No. 1, 10.03.2000, p. 142-150.

    Research output: Contribution to journalArticle

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