We prepared series of P2-P4 domain variants of Escherichia coli ribonuclease P (RNase P) ribozyme, and examined enzymatic activities of them. The results indicated that mutations in these domains did not affect the cleavage site selection of the enzyme, but affected cleavage efficiencies and dependence on magnesium ion concentrations.
|Number of pages||2|
|Journal||Nucleic acids symposium series (2004)|
|Publication status||Published - 2004|
ASJC Scopus subject areas