Mutational analysis on the S-domain of bacterial ribonuclease P ribozyme.

Terumichi Tanaka; Yo Kikuchi

Mutational analysis on the S-domain of bacterial ribonuclease P ribozyme.

Terumichi Tanaka^*, Yo Kikuchi

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

1 Citation (Scopus)

Abstract

We have prepared a temporal engineering template for Escherichia coli RNase P RNA that has additional restriction sites on the S-domain region. We have prepared several deletion variants of S-domain region. The results showed the P12 and P13 domains are necessary for efficient enzymatic reactions and the absence of P14 and P17 domains are easily rescued by the presence of high concentration of magnesium ions.

Original language	English
Pages (from-to)	371-372
Number of pages	2
Journal	Nucleic acids symposium series (2004)
Issue number	51
Publication status	Published - 2007
Externally published	Yes

ASJC Scopus subject areas

Medicine(all)

Cite this

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title = "Mutational analysis on the S-domain of bacterial ribonuclease P ribozyme.",

abstract = "We have prepared a temporal engineering template for Escherichia coli RNase P RNA that has additional restriction sites on the S-domain region. We have prepared several deletion variants of S-domain region. The results showed the P12 and P13 domains are necessary for efficient enzymatic reactions and the absence of P14 and P17 domains are easily rescued by the presence of high concentration of magnesium ions.",

author = "Terumichi Tanaka and Yo Kikuchi",

year = "2007",

language = "English",

pages = "371--372",

journal = "Nucleic acids symposium series (2004)",

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T1 - Mutational analysis on the S-domain of bacterial ribonuclease P ribozyme.

AU - Tanaka, Terumichi

AU - Kikuchi, Yo

PY - 2007

Y1 - 2007

N2 - We have prepared a temporal engineering template for Escherichia coli RNase P RNA that has additional restriction sites on the S-domain region. We have prepared several deletion variants of S-domain region. The results showed the P12 and P13 domains are necessary for efficient enzymatic reactions and the absence of P14 and P17 domains are easily rescued by the presence of high concentration of magnesium ions.

AB - We have prepared a temporal engineering template for Escherichia coli RNase P RNA that has additional restriction sites on the S-domain region. We have prepared several deletion variants of S-domain region. The results showed the P12 and P13 domains are necessary for efficient enzymatic reactions and the absence of P14 and P17 domains are easily rescued by the presence of high concentration of magnesium ions.

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M3 - Article

C2 - 18029741

AN - SCOPUS:42949150257

SN - 1746-8272

SP - 371

EP - 372

JO - Nucleic acids symposium series (2004)

JF - Nucleic acids symposium series (2004)

IS - 51

ER -

Mutational analysis on the S-domain of bacterial ribonuclease P ribozyme.

Abstract

ASJC Scopus subject areas

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