Mutational analysis on the S-domain of bacterial ribonuclease P ribozyme.

Terumichi Tanaka, Yo Kikuchi

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

We have prepared a temporal engineering template for Escherichia coli RNase P RNA that has additional restriction sites on the S-domain region. We have prepared several deletion variants of S-domain region. The results showed the P12 and P13 domains are necessary for efficient enzymatic reactions and the absence of P14 and P17 domains are easily rescued by the presence of high concentration of magnesium ions.

Original languageEnglish
Pages (from-to)371-372
Number of pages2
JournalNucleic acids symposium series (2004)
Issue number51
Publication statusPublished - 2007
Externally publishedYes

Fingerprint

Ribonuclease P
Catalytic RNA
Magnesium
RNA
Ions
Escherichia coli

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Mutational analysis on the S-domain of bacterial ribonuclease P ribozyme. / Tanaka, Terumichi; Kikuchi, Yo.

In: Nucleic acids symposium series (2004), No. 51, 2007, p. 371-372.

Research output: Contribution to journalArticle

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