Mutual reinforcement of inflammation and carcinogenesis by the Helicobacter pylori CagA oncoprotein

Nobumi Suzuki, Naoko Murata-Kamiya, Kohei Yanagiya, Wataru Suda, Masahira Hattori, Hiroaki Kanda, Atsuhiro Bingo, Yumiko Fujii, Shin Maeda, Kazuhiko Koike, Masanori Hatakeyama

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Helicobacter pylori cagA-positive strain delivers the CagA oncoprotein into gastric epithelial cells and at the same time elicits stomach inflammation. To experimentally investigate the pathophysiological interplay between CagA and inflammation, transgenic mice systemically expressing the bacterial cagA gene were treated with a colitis inducer, dextran sulfate sodium (DSS). Compared with control mice, DSS-induced colitis was markedly deteriorated in cagA-transgenic mice. In the colonic epithelia of cagA-transgenic mice, there was a substantial decrease in the level of I? B, which binds and sequesters NF-? B in the cytoplasm. This I? B reduction was due to CagA-mediated inhibition of PAR1, which may stimulate I? B degradation by perturbing microtubule stability. Whereas the CagA-mediated I? B reduction did not automatically activate NF-? B, it lowered the threshold of NF-? B activation by inflammogenic insults, thereby contributing to colitis exacerbation in cagA-transgenic mice. CagA also activates inflammasomes independently of NF-? B signaling, which further potentiates inflammation. The incidence of colonic dysplasia was elevated in DSS-treated cagA-transgenic mice due to a robust increase in the number of pre-cancerous flat-type dysplasias. Thus, CagA deteriorated inflammation, whereas inflammation strengthened the oncogenic potential of CagA. This work revealed that H. pylori CagA and inflammation reinforce each other in creating a downward spiral that instigates neoplastic transformation.

Original languageEnglish
Article number10024
JournalScientific Reports
Volume5
DOIs
Publication statusPublished - 2015 May 6
Externally publishedYes

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Oncogene Proteins
Helicobacter pylori
Carcinogenesis
Transgenic Mice
Inflammation
Dextran Sulfate
Colitis
Stomach
Inflammasomes
Bacterial Genes
Microtubules
Cytoplasm
Epithelium
Epithelial Cells
Incidence

ASJC Scopus subject areas

  • General

Cite this

Suzuki, N., Murata-Kamiya, N., Yanagiya, K., Suda, W., Hattori, M., Kanda, H., ... Hatakeyama, M. (2015). Mutual reinforcement of inflammation and carcinogenesis by the Helicobacter pylori CagA oncoprotein. Scientific Reports, 5, [10024]. https://doi.org/10.1038/srep10024

Mutual reinforcement of inflammation and carcinogenesis by the Helicobacter pylori CagA oncoprotein. / Suzuki, Nobumi; Murata-Kamiya, Naoko; Yanagiya, Kohei; Suda, Wataru; Hattori, Masahira; Kanda, Hiroaki; Bingo, Atsuhiro; Fujii, Yumiko; Maeda, Shin; Koike, Kazuhiko; Hatakeyama, Masanori.

In: Scientific Reports, Vol. 5, 10024, 06.05.2015.

Research output: Contribution to journalArticle

Suzuki, N, Murata-Kamiya, N, Yanagiya, K, Suda, W, Hattori, M, Kanda, H, Bingo, A, Fujii, Y, Maeda, S, Koike, K & Hatakeyama, M 2015, 'Mutual reinforcement of inflammation and carcinogenesis by the Helicobacter pylori CagA oncoprotein', Scientific Reports, vol. 5, 10024. https://doi.org/10.1038/srep10024
Suzuki, Nobumi ; Murata-Kamiya, Naoko ; Yanagiya, Kohei ; Suda, Wataru ; Hattori, Masahira ; Kanda, Hiroaki ; Bingo, Atsuhiro ; Fujii, Yumiko ; Maeda, Shin ; Koike, Kazuhiko ; Hatakeyama, Masanori. / Mutual reinforcement of inflammation and carcinogenesis by the Helicobacter pylori CagA oncoprotein. In: Scientific Reports. 2015 ; Vol. 5.
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