Myosin light chain 2 modulates MgADP-induced contraction in rabbit skeletal and bovine cardiac skinned muscle

Hideaki Fujita, Daisuke Sasaki, Kenji Fukuda, Shin'ichi Ishiwata

    Research output: Contribution to journalArticle

    3 Citations (Scopus)

    Abstract

    Skinned skeletal and cardiac muscle fibres can be activated by MgADP in the presence of MgATP whout Ca2+; the isometric tension is developed in a sigmoidal manner with the addition of MgADP under relaxing conditions. The critical concentrations of MgADP for this MgADP-induced contraction are about 7.5 and 2.6 mM for skeletal and cardiac muscle fibres, respectively. To investigate whether muscle regulatory proteins, myosin light chain 2 (LC2) and troponin C (TnC), play a part in the MgADP-induced contraction, these proteins were partly extracted by treatment with trans-1,2-cyclohexanediamine-N,N,N′,N′-tetraacetic acid (CDTA), a chelater of divalent cations, and the MgADP-tension relationship was examined in rabbit psoas and bovine cardiac skinned fibres. We found that the sigmoidal MgADP-tension relationship became hyperbolic after a partial extraction of LC2 (about 30 %) and TnC (about 70 %). Reconstitution with LC2 restored the sigmoidal MgADP-tension relationship of control fibres almost fully in both skeletal and cardiac fibres, whereas reconstitution with TnC alone had no effect. Furthermore, cardiac fibres reconstituted with skeletal LC2 exhibited an MgADP-tension relationship intermediate between skeletal and cardiac fibres. The partial extraction of LC2 and TnC resulted in a reduction of the inhibitory effect of inorganic phosphate (Pi) on the MgADP-activated tension. Reconstitution with LC2 restored the original Pi-tension relationship, whereas reconstitution with TnC had no effect. In other words, extraction of LC2 apparently increased the affinity of myosin for MgADP but decreased the affinity for Pi. These results demonstrate that LC2 modulates MgADP-induced activation of actomyosin interaction.

    Original languageEnglish
    Pages (from-to)221-229
    Number of pages9
    JournalJournal of Physiology
    Volume542
    Issue number1
    DOIs
    Publication statusPublished - 2002 Jul 1

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    Adenosine Diphosphate
    Myocardium
    Rabbits
    Troponin C
    Light
    Skeletal Muscle Fibers
    myosin light chain 2
    Actomyosin
    Muscle Proteins
    Divalent Cations
    Myosins
    Adenosine Triphosphate
    Phosphates
    Acids

    ASJC Scopus subject areas

    • Physiology

    Cite this

    Myosin light chain 2 modulates MgADP-induced contraction in rabbit skeletal and bovine cardiac skinned muscle. / Fujita, Hideaki; Sasaki, Daisuke; Fukuda, Kenji; Ishiwata, Shin'ichi.

    In: Journal of Physiology, Vol. 542, No. 1, 01.07.2002, p. 221-229.

    Research output: Contribution to journalArticle

    Fujita, Hideaki ; Sasaki, Daisuke ; Fukuda, Kenji ; Ishiwata, Shin'ichi. / Myosin light chain 2 modulates MgADP-induced contraction in rabbit skeletal and bovine cardiac skinned muscle. In: Journal of Physiology. 2002 ; Vol. 542, No. 1. pp. 221-229.
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    abstract = "Skinned skeletal and cardiac muscle fibres can be activated by MgADP in the presence of MgATP whout Ca2+; the isometric tension is developed in a sigmoidal manner with the addition of MgADP under relaxing conditions. The critical concentrations of MgADP for this MgADP-induced contraction are about 7.5 and 2.6 mM for skeletal and cardiac muscle fibres, respectively. To investigate whether muscle regulatory proteins, myosin light chain 2 (LC2) and troponin C (TnC), play a part in the MgADP-induced contraction, these proteins were partly extracted by treatment with trans-1,2-cyclohexanediamine-N,N,N′,N′-tetraacetic acid (CDTA), a chelater of divalent cations, and the MgADP-tension relationship was examined in rabbit psoas and bovine cardiac skinned fibres. We found that the sigmoidal MgADP-tension relationship became hyperbolic after a partial extraction of LC2 (about 30 {\%}) and TnC (about 70 {\%}). Reconstitution with LC2 restored the sigmoidal MgADP-tension relationship of control fibres almost fully in both skeletal and cardiac fibres, whereas reconstitution with TnC alone had no effect. Furthermore, cardiac fibres reconstituted with skeletal LC2 exhibited an MgADP-tension relationship intermediate between skeletal and cardiac fibres. The partial extraction of LC2 and TnC resulted in a reduction of the inhibitory effect of inorganic phosphate (Pi) on the MgADP-activated tension. Reconstitution with LC2 restored the original Pi-tension relationship, whereas reconstitution with TnC had no effect. In other words, extraction of LC2 apparently increased the affinity of myosin for MgADP but decreased the affinity for Pi. These results demonstrate that LC2 modulates MgADP-induced activation of actomyosin interaction.",
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    AB - Skinned skeletal and cardiac muscle fibres can be activated by MgADP in the presence of MgATP whout Ca2+; the isometric tension is developed in a sigmoidal manner with the addition of MgADP under relaxing conditions. The critical concentrations of MgADP for this MgADP-induced contraction are about 7.5 and 2.6 mM for skeletal and cardiac muscle fibres, respectively. To investigate whether muscle regulatory proteins, myosin light chain 2 (LC2) and troponin C (TnC), play a part in the MgADP-induced contraction, these proteins were partly extracted by treatment with trans-1,2-cyclohexanediamine-N,N,N′,N′-tetraacetic acid (CDTA), a chelater of divalent cations, and the MgADP-tension relationship was examined in rabbit psoas and bovine cardiac skinned fibres. We found that the sigmoidal MgADP-tension relationship became hyperbolic after a partial extraction of LC2 (about 30 %) and TnC (about 70 %). Reconstitution with LC2 restored the sigmoidal MgADP-tension relationship of control fibres almost fully in both skeletal and cardiac fibres, whereas reconstitution with TnC alone had no effect. Furthermore, cardiac fibres reconstituted with skeletal LC2 exhibited an MgADP-tension relationship intermediate between skeletal and cardiac fibres. The partial extraction of LC2 and TnC resulted in a reduction of the inhibitory effect of inorganic phosphate (Pi) on the MgADP-activated tension. Reconstitution with LC2 restored the original Pi-tension relationship, whereas reconstitution with TnC had no effect. In other words, extraction of LC2 apparently increased the affinity of myosin for MgADP but decreased the affinity for Pi. These results demonstrate that LC2 modulates MgADP-induced activation of actomyosin interaction.

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