Myotube formation on gelatin nanofibers - Multi-walled carbon nanotubes hybrid scaffolds

Serge Ostrovidov; Xuetao Shi; Ling Zhang; Xiaobin Liang; Sang Bok Kim; Toshinori Fujie; Murugan Ramalingam; Mingwei Chen; Ken Nakajima; Faten Al-Hazmi; Hojae Bae; Adnan Memic; Ali Khademhosseini

doi:10.1016/j.biomaterials.2014.04.021

Myotube formation on gelatin nanofibers - Multi-walled carbon nanotubes hybrid scaffolds

Serge Ostrovidov, Xuetao Shi, Ling Zhang, Xiaobin Liang, Sang Bok Kim, Toshinori Fujie, Murugan Ramalingam, Mingwei Chen, Ken Nakajima, Faten Al-Hazmi, Hojae Bae, Adnan Memic, Ali Khademhosseini^*

^*Corresponding author for this work

Waseda Institute for Advanced Study

Research output: Contribution to journal › Article › peer-review

95 Citations (Scopus)

Abstract

Engineering functional muscle tissue requires the formation of densely packed, aligned, and mature myotubes. To enhance the formation of aligned myotubes with improved contractibility, we fabricated aligned electrospun gelatin multi-walled carbon nanotubes (MWNTs) hybrid fibers that were used as scaffolds for the growth of myoblasts (C2C12). The MWNTs significantly enhanced myotube formation by improving the mechanical properties of the resulting fibers and upregulated the activation of mechanotransduction related genes. In addition, the fibers enhanced the maturation of the myotubes and the amplitude of the myotube contractions under electrical stimulation (ES). Such hybrid material scaffolds may be useful to direct skeletal muscle cellular organization, improve cellular functionality and tissue formation.

Original language	English
Pages (from-to)	6268-6277
Number of pages	10
Journal	Biomaterials
Volume	35
Issue number	24
DOIs	https://doi.org/10.1016/j.biomaterials.2014.04.021
Publication status	Published - 2014

Keywords

C2C12
Carbon nanotubes
Electrospinning
Gelatin fibers
Myotube

ASJC Scopus subject areas

Biomaterials
Bioengineering
Ceramics and Composites
Mechanics of Materials
Biophysics
Medicine(all)

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10.1016/j.biomaterials.2014.04.021

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title = "Myotube formation on gelatin nanofibers - Multi-walled carbon nanotubes hybrid scaffolds",

abstract = "Engineering functional muscle tissue requires the formation of densely packed, aligned, and mature myotubes. To enhance the formation of aligned myotubes with improved contractibility, we fabricated aligned electrospun gelatin multi-walled carbon nanotubes (MWNTs) hybrid fibers that were used as scaffolds for the growth of myoblasts (C2C12). The MWNTs significantly enhanced myotube formation by improving the mechanical properties of the resulting fibers and upregulated the activation of mechanotransduction related genes. In addition, the fibers enhanced the maturation of the myotubes and the amplitude of the myotube contractions under electrical stimulation (ES). Such hybrid material scaffolds may be useful to direct skeletal muscle cellular organization, improve cellular functionality and tissue formation.",

keywords = "C2C12, Carbon nanotubes, Electrospinning, Gelatin fibers, Myotube",

author = "Serge Ostrovidov and Xuetao Shi and Ling Zhang and Xiaobin Liang and Kim, {Sang Bok} and Toshinori Fujie and Murugan Ramalingam and Mingwei Chen and Ken Nakajima and Faten Al-Hazmi and Hojae Bae and Adnan Memic and Ali Khademhosseini",

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T1 - Myotube formation on gelatin nanofibers - Multi-walled carbon nanotubes hybrid scaffolds

AU - Ostrovidov, Serge

AU - Shi, Xuetao

AU - Zhang, Ling

AU - Liang, Xiaobin

AU - Kim, Sang Bok

AU - Fujie, Toshinori

AU - Ramalingam, Murugan

AU - Chen, Mingwei

AU - Nakajima, Ken

AU - Al-Hazmi, Faten

AU - Bae, Hojae

AU - Memic, Adnan

AU - Khademhosseini, Ali

PY - 2014

Y1 - 2014

N2 - Engineering functional muscle tissue requires the formation of densely packed, aligned, and mature myotubes. To enhance the formation of aligned myotubes with improved contractibility, we fabricated aligned electrospun gelatin multi-walled carbon nanotubes (MWNTs) hybrid fibers that were used as scaffolds for the growth of myoblasts (C2C12). The MWNTs significantly enhanced myotube formation by improving the mechanical properties of the resulting fibers and upregulated the activation of mechanotransduction related genes. In addition, the fibers enhanced the maturation of the myotubes and the amplitude of the myotube contractions under electrical stimulation (ES). Such hybrid material scaffolds may be useful to direct skeletal muscle cellular organization, improve cellular functionality and tissue formation.

AB - Engineering functional muscle tissue requires the formation of densely packed, aligned, and mature myotubes. To enhance the formation of aligned myotubes with improved contractibility, we fabricated aligned electrospun gelatin multi-walled carbon nanotubes (MWNTs) hybrid fibers that were used as scaffolds for the growth of myoblasts (C2C12). The MWNTs significantly enhanced myotube formation by improving the mechanical properties of the resulting fibers and upregulated the activation of mechanotransduction related genes. In addition, the fibers enhanced the maturation of the myotubes and the amplitude of the myotube contractions under electrical stimulation (ES). Such hybrid material scaffolds may be useful to direct skeletal muscle cellular organization, improve cellular functionality and tissue formation.

KW - C2C12

KW - Carbon nanotubes

KW - Electrospinning

KW - Gelatin fibers

KW - Myotube

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EP - 6277

JO - Biomaterials

JF - Biomaterials

IS - 24

ER -

Myotube formation on gelatin nanofibers - Multi-walled carbon nanotubes hybrid scaffolds

Abstract

Keywords

ASJC Scopus subject areas

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