NaF activates MAPKs and induces apoptosis in odontoblast-like cells

H. Karube, G. Nishitai, K. Inageda, H. Kurosu, M. Matsuoka

Research output: Contribution to journalArticle

68 Citations (Scopus)

Abstract

The cytotoxic effects of fluoride on odontoblasts are not clear. In this study, we examined whether NaF induces apoptosis in MDPC-23 odontoblast-like cells and the involvement of mitogen-activated protein kinase (MAPK) signaling pathways in NaF-induced apoptosis. MDPC-23 cells incubated with 5 mM NaF for 24 hrs exhibited caspase-3 activation, cleavage of poly(ADP-ribose) polymerase, DNA fragmentation, and an increase in cytoplasmic nucleosomes. Prior to the induction of apoptosis, all MAPKs examined were phosphorylated, but in a different manner. In contrast to the sustained phosphorylation of c-Jun NH2-terminal kinase (JNK) and p38, NaF exposure induced a biphasic phosphorylation of extracellular signal-regulated protein kinase (ERK). NaF-induced apoptosis was markedly suppressed by treatment with the JNK inhibitor, SP600125, and mildly suppressed by the MAPK/ERK kinase inhibitor, U0126. Inhibition of p38 activity did not protect cells from apoptosis. Thus, exposure to NaF induces apoptosis in odontoblast-like cells, depending on JNK and, less significantly, ERK pathways.

Original languageEnglish
Pages (from-to)461-465
Number of pages5
JournalJournal of Dental Research
Volume88
Issue number5
DOIs
Publication statusPublished - 2009 May 1
Externally publishedYes

Fingerprint

Odontoblasts
Apoptosis
JNK Mitogen-Activated Protein Kinases
Phosphorylation
Poly(ADP-ribose) Polymerases
MAP Kinase Signaling System
Nucleosomes
Mitogen-Activated Protein Kinase Kinases
Extracellular Signal-Regulated MAP Kinases
DNA Fragmentation
Mitogen-Activated Protein Kinases
Fluorides
Caspase 3
Protein Kinases

Keywords

  • Apoptosis
  • MAPKs
  • MDPC-23 cells
  • Odontoblasts
  • Sodium fluoride

ASJC Scopus subject areas

  • Dentistry(all)

Cite this

Karube, H., Nishitai, G., Inageda, K., Kurosu, H., & Matsuoka, M. (2009). NaF activates MAPKs and induces apoptosis in odontoblast-like cells. Journal of Dental Research, 88(5), 461-465. https://doi.org/10.1177/0022034509334771

NaF activates MAPKs and induces apoptosis in odontoblast-like cells. / Karube, H.; Nishitai, G.; Inageda, K.; Kurosu, H.; Matsuoka, M.

In: Journal of Dental Research, Vol. 88, No. 5, 01.05.2009, p. 461-465.

Research output: Contribution to journalArticle

Karube, H, Nishitai, G, Inageda, K, Kurosu, H & Matsuoka, M 2009, 'NaF activates MAPKs and induces apoptosis in odontoblast-like cells', Journal of Dental Research, vol. 88, no. 5, pp. 461-465. https://doi.org/10.1177/0022034509334771
Karube, H. ; Nishitai, G. ; Inageda, K. ; Kurosu, H. ; Matsuoka, M. / NaF activates MAPKs and induces apoptosis in odontoblast-like cells. In: Journal of Dental Research. 2009 ; Vol. 88, No. 5. pp. 461-465.
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