Nanaomycin K, a new epithelial–mesenchymal transition inhibitor produced by the actinomycete “Streptomyces rosa subsp. notoensis” OS-3966

Hirotaka Matsuo, Jun Nakanishi, Yoshihiko Noguchi, Koichi Kitagawa, Katsumi Shigemura, Toshiaki Sunazuka, Yōko Takahashi, Satoshi Ōmura, Takuji Nakashima

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

A new nanaomycin analog, nanaomycin K, was isolated from a cultured broth of actinomycete strain “Streptomyces rosa subsp. notoensis” OS-3966. Nuclear magnetic resonance (NMR) analyses revealed that the planar structure of nanaomycin K had an ergothioneine moiety. To determine the absolute configuration, nanaomycin K was semisynthesized using standards of nanaomycin E and L-ergothioneine. The natural and semisynthetic nanaomycin K were identified as the same compounds based on retention time, mass spectrometry, 1H NMR, and optical rotation data. Nanaomycin K showed cytotoxicity against Madin–Darby canine kidney (MDCK) cells undergoing transforming growth factor (TGF) β1-induced epithelial–mesenchymal transition.

Original languageEnglish
Pages (from-to)291-295
Number of pages5
JournalJournal of Bioscience and Bioengineering
Volume129
Issue number3
DOIs
Publication statusPublished - 2020 Mar
Externally publishedYes

Keywords

  • Actinomycete
  • Epithelial–mesenchymal transition
  • Ergothioneine
  • Nanaomycin K
  • Transforming growth factor β1

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

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