Abstract
We designed and synthesized dumbbell-shaped nanocircular RNAs for RNA interference applications, which consist of a stem and two loops(1). RNA dumbbells are specifically recognized and cleaved by the human Dicer enzyme, and are thus transformed into double-stranded RNA in cells, although this RNA is resistant to degradation in serum. The structure was optimized to maximize its RNAi activity. The most potent activity was achieved when the stem length was 23 base pairs. The RNAi activity is prolonged by the shape of the molecule, an endless structure, compared with that of normal siRNA.
| Original language | English |
|---|---|
| Pages (from-to) | 505-506 |
| Number of pages | 2 |
| Journal | Nucleic acids symposium series (2004) |
| Issue number | 52 |
| Publication status | Published - 2008 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Medicine(all)
Cite this
Nanocircular RNAs for RNA interference. / Abe, Hiroshi; Abe, Naoko; Harada, Mitsuru; Tsuneda, Satoshi; Ito, Yoshihiro.
In: Nucleic acids symposium series (2004), No. 52, 2008, p. 505-506.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Nanocircular RNAs for RNA interference.
AU - Abe, Hiroshi
AU - Abe, Naoko
AU - Harada, Mitsuru
AU - Tsuneda, Satoshi
AU - Ito, Yoshihiro
PY - 2008
Y1 - 2008
N2 - We designed and synthesized dumbbell-shaped nanocircular RNAs for RNA interference applications, which consist of a stem and two loops(1). RNA dumbbells are specifically recognized and cleaved by the human Dicer enzyme, and are thus transformed into double-stranded RNA in cells, although this RNA is resistant to degradation in serum. The structure was optimized to maximize its RNAi activity. The most potent activity was achieved when the stem length was 23 base pairs. The RNAi activity is prolonged by the shape of the molecule, an endless structure, compared with that of normal siRNA.
AB - We designed and synthesized dumbbell-shaped nanocircular RNAs for RNA interference applications, which consist of a stem and two loops(1). RNA dumbbells are specifically recognized and cleaved by the human Dicer enzyme, and are thus transformed into double-stranded RNA in cells, although this RNA is resistant to degradation in serum. The structure was optimized to maximize its RNAi activity. The most potent activity was achieved when the stem length was 23 base pairs. The RNAi activity is prolonged by the shape of the molecule, an endless structure, compared with that of normal siRNA.
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UR - http://www.scopus.com/inward/citedby.url?scp=78649403330&partnerID=8YFLogxK
M3 - Article
C2 - 18776475
AN - SCOPUS:78649403330
SP - 505
EP - 506
JO - Nucleic acids symposium series (2004)
JF - Nucleic acids symposium series (2004)
SN - 1746-8272
IS - 52
ER -