Nanocircular RNAs for RNA interference.

Hiroshi Abe; Naoko Abe; Mitsuru Harada; Satoshi Tsuneda; Yoshihiro Ito

Nanocircular RNAs for RNA interference.

Hiroshi Abe, Naoko Abe, Mitsuru Harada, Satoshi Tsuneda, Yoshihiro Ito

Research output: Contribution to journal › Article › peer-review

Abstract

We designed and synthesized dumbbell-shaped nanocircular RNAs for RNA interference applications, which consist of a stem and two loops(1). RNA dumbbells are specifically recognized and cleaved by the human Dicer enzyme, and are thus transformed into double-stranded RNA in cells, although this RNA is resistant to degradation in serum. The structure was optimized to maximize its RNAi activity. The most potent activity was achieved when the stem length was 23 base pairs. The RNAi activity is prolonged by the shape of the molecule, an endless structure, compared with that of normal siRNA.

Original language	English
Pages (from-to)	505-506
Number of pages	2
Journal	Nucleic acids symposium series (2004)
Issue number	52
Publication status	Published - 2008 Dec 1
Externally published	Yes

ASJC Scopus subject areas

Medicine(all)

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AU - Harada, Mitsuru

AU - Tsuneda, Satoshi

AU - Ito, Yoshihiro

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AB - We designed and synthesized dumbbell-shaped nanocircular RNAs for RNA interference applications, which consist of a stem and two loops(1). RNA dumbbells are specifically recognized and cleaved by the human Dicer enzyme, and are thus transformed into double-stranded RNA in cells, although this RNA is resistant to degradation in serum. The structure was optimized to maximize its RNAi activity. The most potent activity was achieved when the stem length was 23 base pairs. The RNAi activity is prolonged by the shape of the molecule, an endless structure, compared with that of normal siRNA.

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