Nanoscale patterning of protein using electron beam lithography of organosilane self-assembled monolayers

Guo Jun Zhang, Takashi Tanii, Tamotsu Zako, Takumi Hosaka, Takeo Miyuke, Yuzo Kanari, Takashi Funatsu, Iwao Ohdomari

Research output: Contribution to journalArticle

70 Citations (Scopus)

Abstract

Nanopatterning of self-assembled monolayers (SAM) by electron beam lithography (EB) was developed to produce organic templates for various nanofabrication processes. EB lithography of SAMs provide a method of patterning substrates with proteins with ultrahigh resolution down to sub-10 nm on the scale of single molecule. Green fluorescent proteins (GFP), which is a spontaneous fluorescence protein isolated from the Pacific Jellyfish was chosen as a model protein. A cysteine residue at the c-terminal end of the protein by site-directed mutagenesis for the site specific modification of protein with biotin was introduced. Field-emission scanning electron microscopy was employed to observe nanostructures composed of two different types of organosilane layers. EB lithography of fluorinated SAM as an ideal template enables the high-resolution patterning of proteins with a high signal-to-noise ratio and integration of bioactive proteins into biosensors.

Original languageEnglish
Pages (from-to)833-837
Number of pages5
JournalSmall
Volume1
Issue number8-9
DOIs
Publication statusPublished - 2005 Aug 1

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Keywords

  • Biorecognition
  • Nanolithography
  • Patterning
  • Protein design
  • Self-assembled monolayers

ASJC Scopus subject areas

  • Biotechnology
  • Biomaterials
  • Chemistry(all)
  • Materials Science(all)

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