Nanosecond fluorometric investigation of hydrodynamic properties of adenosine triphosphatase from thermophilic bacterium PS3

Kazuhiko Kinosita, Akira Ikegami, Masasuke Yoshida, Yasuo Kagawa

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

The soluble portion (TF1) of proton-translocating ATPase from thermophilic bacterium PS3 was labeled with a fluorescent dye N-(1-pyrene)maleimide. The decay of fluorescence anisotropy of the adduct showed that TF1 in aqueous solution was characterized by a volume of equivalent sphere of 1, 120 nm3. This value is 2.4 times the volume calculated from the molecular weight and partial specific volume, indicating a non-spherical shape and/or extensive hydration. A prolate ellipsoid with an axial ratio of 2 to 3 is suggested as a first approximation of the shape of hydrated TF1. The presence or absence of ATP, ADP, or Mg2+ did not alter the volume of the equivalent sphere appreciably; the probable conformational change of TF1 induced by these ligands does not lead to a gross alteration of its hydrody-namic properties.

Original languageEnglish
Pages (from-to)2043-2046
Number of pages4
JournalJournal of Biochemistry
Volume92
Issue number6
Publication statusPublished - 1982 Oct
Externally publishedYes

Fingerprint

Phosmet
Fluorescence Polarization
Proton-Translocating ATPases
Adenosine
Hydrodynamics
Fluorescent Dyes
Bacteria
Adenosine Diphosphate
Adenosine Triphosphatases
Adenosine Triphosphate
Molecular Weight
Ligands
Administrative data processing
Adenosinetriphosphate
Pyrene
Hydration
Density (specific gravity)
Prolate ellipsoid
Protons
Anisotropy

ASJC Scopus subject areas

  • Statistics, Probability and Uncertainty
  • Applied Mathematics
  • Physiology (medical)
  • Radiology Nuclear Medicine and imaging
  • Molecular Biology
  • Biochemistry

Cite this

Nanosecond fluorometric investigation of hydrodynamic properties of adenosine triphosphatase from thermophilic bacterium PS3. / Kinosita, Kazuhiko; Ikegami, Akira; Yoshida, Masasuke; Kagawa, Yasuo.

In: Journal of Biochemistry, Vol. 92, No. 6, 10.1982, p. 2043-2046.

Research output: Contribution to journalArticle

Kinosita, Kazuhiko ; Ikegami, Akira ; Yoshida, Masasuke ; Kagawa, Yasuo. / Nanosecond fluorometric investigation of hydrodynamic properties of adenosine triphosphatase from thermophilic bacterium PS3. In: Journal of Biochemistry. 1982 ; Vol. 92, No. 6. pp. 2043-2046.
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