Nanosecond time-resolved fluorescence investigations of temperature-induced conformational changes in cytochrome oxidase in phosphatidylcholine vesicles and solubilized systems

Suguru Kawato, Satoshi Yoshida, Yutaka Orii, Akira Ikegami, Kazuhiko Kinosita

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Intrinsic and lipid phase transition-induced conformational changes in cytochrome oxidase in phosphatidylcholine vesicle and solubilized systems were examined by the fluorescence lifetime of N-(1-anilinonaphthyl-4)-maleimide conjugated with the enzyme. The time-dependent fluorescence intensity of N-(1-anilinonaphthyl-4)-maleimide attached to cytochrome oxidase was described as a triple exponential decay. Both the intrinsic and lipid phase transition-induced conformational changes were detectable in plots of the average lifetime against temperature. In most cases a peak occurred at the temperature of the conformational change. The time-dependent emission anisotropy showed that N-(1-anilinonaphthyl-4)-maleimide embedded in cytochrome oxidase in phosphatidylcholine vesicles underwent a rapid restricted wobbling within a cone. The half-angle of the cone was around 30° for cytochrome oxidase in dimyristoyl phosphatidylcholine vesicles.

Original languageEnglish
Pages (from-to)85-92
Number of pages8
JournalBBA - Bioenergetics
Volume634
Issue numberC
DOIs
Publication statusPublished - 1981
Externally publishedYes

Keywords

  • (Nanosecond spectroscopy)
  • Conformational change
  • Cytochrome oxidase
  • Fluorescence anisotropy
  • Phase transition
  • Phosphatidylcholine vesicle

ASJC Scopus subject areas

  • Biophysics
  • Medicine(all)

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