Nanostructures control the hepatocellular responses to a cytotoxic agent "cisplatin"

Shimaa A. Abdellatef, Riho Tange, Takeshi Sato, Akihiko Ohi, Toshihide Nabatame, Akiyoshi Taniguchi

    Research output: Contribution to journalArticle

    2 Citations (Scopus)

    Abstract

    In drug discovery programs, the alteration between in vivo and in vitro cellular responses to drug represents one of the main challenges. Since the variation in the native extracellular matrix (ECM) between in vivo and 2D in vitro conditions is one of the key reasons for such discrepancies, thus the utilization of substrate that likely mimics ECM characteristics (topography, stiffness, and chemical composition) is needed to overcome such problem. Here, we investigated the role of substrate nanotopography as one of the major determinants of hepatic cellular responses to a chemotherapeutic agent "cisplatin." We studied the substratum induced variations in cisplatin cytotoxicity; a higher cytotoxic response to cisplatin was observed for cells cultured on the nanopattern relative to a flat substrate. Moreover, the nanofeatures with grating shapes that mimic the topography of major ECM protein constituents (collagen) induced alterations in the cellular orientation and chromatin condensation compared to flat surfaces. Accordingly, the developments of biomimetic substrates with a particular topography could have potentials in drug development analyses to reflect more physiological mimicry conditions in vitro.

    Original languageEnglish
    Article number925319
    JournalBioMed Research International
    Volume2015
    DOIs
    Publication statusPublished - 2015

    Fingerprint

    Nanostructures
    Cytotoxins
    Cisplatin
    Topography
    Extracellular Matrix
    Substrates
    Biomimetics
    Extracellular Matrix Proteins
    Drug Discovery
    Pharmaceutical Preparations
    Chromatin
    Cultured Cells
    Collagen
    Cytotoxicity
    Condensation
    Liver
    Stiffness
    In Vitro Techniques
    Chemical analysis

    ASJC Scopus subject areas

    • Biochemistry, Genetics and Molecular Biology(all)
    • Immunology and Microbiology(all)

    Cite this

    Nanostructures control the hepatocellular responses to a cytotoxic agent "cisplatin". / Abdellatef, Shimaa A.; Tange, Riho; Sato, Takeshi; Ohi, Akihiko; Nabatame, Toshihide; Taniguchi, Akiyoshi.

    In: BioMed Research International, Vol. 2015, 925319, 2015.

    Research output: Contribution to journalArticle

    Abdellatef, Shimaa A. ; Tange, Riho ; Sato, Takeshi ; Ohi, Akihiko ; Nabatame, Toshihide ; Taniguchi, Akiyoshi. / Nanostructures control the hepatocellular responses to a cytotoxic agent "cisplatin". In: BioMed Research International. 2015 ; Vol. 2015.
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    AU - Sato, Takeshi

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    AU - Nabatame, Toshihide

    AU - Taniguchi, Akiyoshi

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