Navigating the folding energy landscape of green fluorescent protein

Morten Bertz, Andrea Kunfermann, Matthias Rief

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

The directed application of force to different points on the surface of the green fluorescent protein (GFP) makes it possible to shift between two different pathways with distinct unfolding intermediates. One pathway resembles a "folding-like" pathway, whereas the other may play a role during processes such as import through a pore. The picture shows the energy landscape of GFP with intermediates and pathways. (Figure Presented)

Original languageEnglish
Pages (from-to)8192-8195
Number of pages4
JournalAngewandte Chemie - International Edition
Volume47
Issue number43
DOIs
Publication statusPublished - 2008 Oct 13
Externally publishedYes

Fingerprint

Green Fluorescent Proteins
Proteins

Keywords

  • Protein engineering
  • Protein folding
  • Protein structures
  • Scanning probe microscopy
  • Single-molecule studies

ASJC Scopus subject areas

  • Chemistry(all)
  • Catalysis

Cite this

Navigating the folding energy landscape of green fluorescent protein. / Bertz, Morten; Kunfermann, Andrea; Rief, Matthias.

In: Angewandte Chemie - International Edition, Vol. 47, No. 43, 13.10.2008, p. 8192-8195.

Research output: Contribution to journalArticle

Bertz, Morten ; Kunfermann, Andrea ; Rief, Matthias. / Navigating the folding energy landscape of green fluorescent protein. In: Angewandte Chemie - International Edition. 2008 ; Vol. 47, No. 43. pp. 8192-8195.
@article{79653f6cc9b846199d78fa66cc3b6b1f,
title = "Navigating the folding energy landscape of green fluorescent protein",
abstract = "The directed application of force to different points on the surface of the green fluorescent protein (GFP) makes it possible to shift between two different pathways with distinct unfolding intermediates. One pathway resembles a {"}folding-like{"} pathway, whereas the other may play a role during processes such as import through a pore. The picture shows the energy landscape of GFP with intermediates and pathways. (Figure Presented)",
keywords = "Protein engineering, Protein folding, Protein structures, Scanning probe microscopy, Single-molecule studies",
author = "Morten Bertz and Andrea Kunfermann and Matthias Rief",
year = "2008",
month = "10",
day = "13",
doi = "10.1002/anie.200802987",
language = "English",
volume = "47",
pages = "8192--8195",
journal = "Angewandte Chemie - International Edition",
issn = "1433-7851",
publisher = "John Wiley and Sons Ltd",
number = "43",

}

TY - JOUR

T1 - Navigating the folding energy landscape of green fluorescent protein

AU - Bertz, Morten

AU - Kunfermann, Andrea

AU - Rief, Matthias

PY - 2008/10/13

Y1 - 2008/10/13

N2 - The directed application of force to different points on the surface of the green fluorescent protein (GFP) makes it possible to shift between two different pathways with distinct unfolding intermediates. One pathway resembles a "folding-like" pathway, whereas the other may play a role during processes such as import through a pore. The picture shows the energy landscape of GFP with intermediates and pathways. (Figure Presented)

AB - The directed application of force to different points on the surface of the green fluorescent protein (GFP) makes it possible to shift between two different pathways with distinct unfolding intermediates. One pathway resembles a "folding-like" pathway, whereas the other may play a role during processes such as import through a pore. The picture shows the energy landscape of GFP with intermediates and pathways. (Figure Presented)

KW - Protein engineering

KW - Protein folding

KW - Protein structures

KW - Scanning probe microscopy

KW - Single-molecule studies

UR - http://www.scopus.com/inward/record.url?scp=54249156909&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=54249156909&partnerID=8YFLogxK

U2 - 10.1002/anie.200802987

DO - 10.1002/anie.200802987

M3 - Article

VL - 47

SP - 8192

EP - 8195

JO - Angewandte Chemie - International Edition

JF - Angewandte Chemie - International Edition

SN - 1433-7851

IS - 43

ER -