Neurogenic potential of progenitors derived from human circulating CD14+ monocytes

Hiroaki Kodama, Takafumi Inoue, Ryuichi Watanabe, Daisuke Yasutomi, Yutaka Kawakami, Satoshi Ogawa, Katsuhiko Mikoshiba, Yasuo Ikeda, Masataka Kuwana

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

We previously reported a primitive cell fraction derived from human circulating CD14+ monocytes, named monocyte-derived multipotential cells (MOMC), that can differentiate along mesenchymal lineages, including bone, cartilage, fat, skeletal muscle and cardiac muscle. In this study, we investigated whether MOMC can differentiate into the neuronal lineage. MOMC were fluorescently labelled and cocultivated with a primary culture of rat neurons for up to 4 weeks. The protein and gene expressions of neuron-specific markers in the human MOMC were evaluated over time using immunohistochemistry, in situ hybridization and reverse transcription followed by PCR. Shortly after cocultivation with rat neurons, nearly all the MOMC expressed early neuroectodermal markers, Mash1, Neurogenin2 and NeuroD, together with nestin, an intermediate filament expressed in neurogenesis. After 14 days of coculture, a subpopulation of MOMC displayed a multipolar morphology with elongated neurites and expressed mature neuron-specific markers, including neurofilament, microtubule-associated protein type 2, β3-tubulin, NeuN and Hu. Transdifferentiation of monocytes into the neuroectodermal lineage was shown by the simultaneous expression of proneural markers and CD45/CD14 early in the differentiation process. The cocultivated MOMC retained their proliferative capacity for at least 16 days. Finally, the neuronal differentiation of MOMC was observed when they were cultured with neurons without cell-to-cell contact. The capacity of MOMC to differentiate into both mesodermal and neuroectodermal lineages suggests that circulating CD14+ monocytes are more multipotential than previously thought.

Original languageEnglish
Pages (from-to)209-217
Number of pages9
JournalImmunology and Cell Biology
Volume84
Issue number2
DOIs
Publication statusPublished - 2006 Apr
Externally publishedYes

Fingerprint

Neurons
Monocytes
Muscle
Rats
Nestin
Microtubule-Associated Proteins
Cartilage
Transcription
Tubulin
Intermediate Filaments
Gene expression
Coculture Techniques
Bone
Fats
Neurogenesis
Neurites
Reverse Transcription
In Situ Hybridization
Myocardium
Skeletal Muscle

Keywords

  • Monocyte
  • Monocyte-derived multipotential cell
  • Neuron
  • Regeneration
  • Transdifferentiation

ASJC Scopus subject areas

  • Immunology
  • Clinical Biochemistry
  • Cell Biology

Cite this

Neurogenic potential of progenitors derived from human circulating CD14+ monocytes. / Kodama, Hiroaki; Inoue, Takafumi; Watanabe, Ryuichi; Yasutomi, Daisuke; Kawakami, Yutaka; Ogawa, Satoshi; Mikoshiba, Katsuhiko; Ikeda, Yasuo; Kuwana, Masataka.

In: Immunology and Cell Biology, Vol. 84, No. 2, 04.2006, p. 209-217.

Research output: Contribution to journalArticle

Kodama, H, Inoue, T, Watanabe, R, Yasutomi, D, Kawakami, Y, Ogawa, S, Mikoshiba, K, Ikeda, Y & Kuwana, M 2006, 'Neurogenic potential of progenitors derived from human circulating CD14+ monocytes', Immunology and Cell Biology, vol. 84, no. 2, pp. 209-217. https://doi.org/10.1111/j.1440-1711.2006.01424.x
Kodama, Hiroaki ; Inoue, Takafumi ; Watanabe, Ryuichi ; Yasutomi, Daisuke ; Kawakami, Yutaka ; Ogawa, Satoshi ; Mikoshiba, Katsuhiko ; Ikeda, Yasuo ; Kuwana, Masataka. / Neurogenic potential of progenitors derived from human circulating CD14+ monocytes. In: Immunology and Cell Biology. 2006 ; Vol. 84, No. 2. pp. 209-217.
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