Neutrophilic cell production by combination of stem cell factor and thrombopoietin from CD34+ cord blood cells in long-term serum-deprived liquid culture

Nobukuni Sawai, Kenichi Koike, Susumu Ito, Hadija Hemed Mwamtemi, Yumi Kurokawa, Tatsuya Kinoshita, Kazuo Sakashita, Tsukasa Higuchi, Kouichi Takeuchi, Masaaki Shiohara, Hiroshi Miyazaki, Takashi Kato, Atsushi Komiyama

Research output: Contribution to journalArticle

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Abstract

In the present study, we investigated the effects of stem cell factor (SCF) and/or thrombopoietin (TPO) on the cell production by cord blood CD34+ cells using a serum-deprived liquid culture system. Although SCF alone supported a modest production of neutrophilic cells and a remarkable generation of mast cells, the addition of TPO to the culture containing SCF caused an apparent generation of neutrophilic cells, identified by immunocytochemical staining and flow cytometric analysis. The significant production of neutrophilic cells by SCF and TPO was persistently observed from 2 weeks to 2 to 3 months of culture. The interaction between SCF and TPO on the neutrophilic cell generation was greater than the combined effects of SCF with granulocyte colony-stimulating factor (G-CSF) or granulocyte- macrophage colony-stimulating factor (GM-CSF). The addition of neutralizing antibody against G-CSF or GM-CSF did not influence the SCF + TPO-dependent neutrophilic cell production. A single-cell culture study showed that not only CD34+CD38+c-kit+ cells but also CD34+CD38-c-kit+ cells were responsible for the neutrophilic cell generation. In clonal cell cultures, GM progenitors as well as erythroid progenitors and multipotential progenitors expanded in the cultures supplemented with SCF and TPO. The neutrophilic cells grown by SCF + TPO were at myeloblast to band cell stages, and scarcely matured to segmented neutrophils. In addition, the cells generated by SCF + TPO were stained with monoclonal antibodies against myeloperoxidase, elastase, lactoferrin, and CD11b, but they had negligible levels of alkaline phosphatase (ALP) and CD35. The replating of the CD34-c-kit(-/low) CD15+ cells grown by SCF + TPO into a culture containing SCF + G-CSF permitted both the terminal maturation into segmented cells and the appearance of ALP and CD35. These results indicate the existence of a G-CSF/GM-CSF-independent system of neutrophilic cell production.

Original languageEnglish
Pages (from-to)509-518
Number of pages10
JournalBlood
Volume93
Issue number2
Publication statusPublished - 1999 Jan 15
Externally publishedYes

Fingerprint

Thrombopoietin
Stem Cell Factor
Fetal Blood
Cell culture
Blood Cells
Blood
Cells
Liquids
Serum
Granulocyte Colony-Stimulating Factor
Granulocyte-Macrophage Colony-Stimulating Factor
Alkaline Phosphatase
Cell Culture Techniques
Lactoferrin
Pancreatic Elastase
Granulocyte Precursor Cells
Neutralizing Antibodies
Peroxidase

ASJC Scopus subject areas

  • Hematology

Cite this

Sawai, N., Koike, K., Ito, S., Mwamtemi, H. H., Kurokawa, Y., Kinoshita, T., ... Komiyama, A. (1999). Neutrophilic cell production by combination of stem cell factor and thrombopoietin from CD34+ cord blood cells in long-term serum-deprived liquid culture. Blood, 93(2), 509-518.

Neutrophilic cell production by combination of stem cell factor and thrombopoietin from CD34+ cord blood cells in long-term serum-deprived liquid culture. / Sawai, Nobukuni; Koike, Kenichi; Ito, Susumu; Mwamtemi, Hadija Hemed; Kurokawa, Yumi; Kinoshita, Tatsuya; Sakashita, Kazuo; Higuchi, Tsukasa; Takeuchi, Kouichi; Shiohara, Masaaki; Miyazaki, Hiroshi; Kato, Takashi; Komiyama, Atsushi.

In: Blood, Vol. 93, No. 2, 15.01.1999, p. 509-518.

Research output: Contribution to journalArticle

Sawai, N, Koike, K, Ito, S, Mwamtemi, HH, Kurokawa, Y, Kinoshita, T, Sakashita, K, Higuchi, T, Takeuchi, K, Shiohara, M, Miyazaki, H, Kato, T & Komiyama, A 1999, 'Neutrophilic cell production by combination of stem cell factor and thrombopoietin from CD34+ cord blood cells in long-term serum-deprived liquid culture', Blood, vol. 93, no. 2, pp. 509-518.
Sawai, Nobukuni ; Koike, Kenichi ; Ito, Susumu ; Mwamtemi, Hadija Hemed ; Kurokawa, Yumi ; Kinoshita, Tatsuya ; Sakashita, Kazuo ; Higuchi, Tsukasa ; Takeuchi, Kouichi ; Shiohara, Masaaki ; Miyazaki, Hiroshi ; Kato, Takashi ; Komiyama, Atsushi. / Neutrophilic cell production by combination of stem cell factor and thrombopoietin from CD34+ cord blood cells in long-term serum-deprived liquid culture. In: Blood. 1999 ; Vol. 93, No. 2. pp. 509-518.
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abstract = "In the present study, we investigated the effects of stem cell factor (SCF) and/or thrombopoietin (TPO) on the cell production by cord blood CD34+ cells using a serum-deprived liquid culture system. Although SCF alone supported a modest production of neutrophilic cells and a remarkable generation of mast cells, the addition of TPO to the culture containing SCF caused an apparent generation of neutrophilic cells, identified by immunocytochemical staining and flow cytometric analysis. The significant production of neutrophilic cells by SCF and TPO was persistently observed from 2 weeks to 2 to 3 months of culture. The interaction between SCF and TPO on the neutrophilic cell generation was greater than the combined effects of SCF with granulocyte colony-stimulating factor (G-CSF) or granulocyte- macrophage colony-stimulating factor (GM-CSF). The addition of neutralizing antibody against G-CSF or GM-CSF did not influence the SCF + TPO-dependent neutrophilic cell production. A single-cell culture study showed that not only CD34+CD38+c-kit+ cells but also CD34+CD38-c-kit+ cells were responsible for the neutrophilic cell generation. In clonal cell cultures, GM progenitors as well as erythroid progenitors and multipotential progenitors expanded in the cultures supplemented with SCF and TPO. The neutrophilic cells grown by SCF + TPO were at myeloblast to band cell stages, and scarcely matured to segmented neutrophils. In addition, the cells generated by SCF + TPO were stained with monoclonal antibodies against myeloperoxidase, elastase, lactoferrin, and CD11b, but they had negligible levels of alkaline phosphatase (ALP) and CD35. The replating of the CD34-c-kit(-/low) CD15+ cells grown by SCF + TPO into a culture containing SCF + G-CSF permitted both the terminal maturation into segmented cells and the appearance of ALP and CD35. These results indicate the existence of a G-CSF/GM-CSF-independent system of neutrophilic cell production.",
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AU - Mwamtemi, Hadija Hemed

AU - Kurokawa, Yumi

AU - Kinoshita, Tatsuya

AU - Sakashita, Kazuo

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