New generation of hemoglobin-based oxygen carriers evaluated for oxygenation of critically ischemic hamster flap tissue

Claudio Contaldo*, Jan Plock, Hiromi Sakai, Shinji Takeoka, Eishun Tsuchida, Michael Leunig, Andrej Banic, Dominique Erni

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

25 Citations (Scopus)


Objectives: The aim of this study was to investigate and compare the effects of a traditionally formulated, low-viscosity, right-shifted polymerized bovine hemoglobin solution and a highly viscous, left-shifted hemoglobin vesicle solution (HbV-HES) on the oxygenation of critically ischemic peripheral tissue. Design: Randomized, prospective study. Setting: University laboratory. Subject: A total of 40 male golden Syrian hamsters. Interventions: Island flaps were dissected from the back skin of anesthetized hamsters. The flap included a critically ischemic, hypoxic area that was perfused via a collateralized vasculature. One hour after completion of the preparation, the animals received a 33% blood exchange with 6% hydroxyethyl starch 200/ 0.5 (HES, n = 9), HbV suspended in HES (HbV-HES, n = 8), or polymerized bovine hemoglobin solution (n = 9). Measurements and Main Results: Three hours after the blood exchange, microcirculatory blood flow (laser-Doppler flowmetry) was increased to 262% of baseline for HbV-HES (p < .01) and 197% for polymerized bovine hemoglobin solution (p < .05 vs. baseline and HbV-HES). Partial tissue oxygen tension (bare fiber probes) was only improved after HbV-HES (9.4 torr to 14.2 torr, p < .01 vs. baseline and other groups). The tissue lactate/pyruvate ratio (microdialysis) was elevated to 51 in the untreated control animals, and to 34 ± 8 after HbV-HES (p < .05 vs. control) and 38 ± 11 after polymerized bovine hemoglobin solution (not significant). Conclusions: Our study suggests that in critically ischemic and hypoxic collateralized peripheral tissue, oxygenation may be improved by normovolemic hemodilution with HbV-HES. We attributed this improvement to a better restoration of the microcirculation and oxygen delivery due to the formulation of the solution.

Original languageEnglish
Pages (from-to)806-812
Number of pages7
JournalCritical Care Medicine
Issue number4
Publication statusPublished - 2005 Apr 1


  • Arterial occlusive diseases
  • Blood substitutes
  • Collateral circulation
  • Energy metabolism
  • Microcirculation
  • Surgical flaps

ASJC Scopus subject areas

  • Critical Care and Intensive Care Medicine


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