New human myelodysplastic cell line, TER-3: G-CSF specific downregulation of Ca 2+/calmodulin-dependent protein kinase IV

Yuji Mishima, Yasuhito Terui, Yuko Mishima, Misa Katsuyama, Masaki Mori, Hiroshi Tomizuka, Toshiyuki Takizawa, Akira Miyazato, Masuzu Ueda, Muneo Yamada, Hirotoshi Hayasawa, Nobuyuki Mizunuma, Yukihito Ishizaka, Kazuma Ikeda, Takashi Kato, Keiya Ozawa, Kiyohiko Hatake

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

We have established a new hematopoietic cell line from a patient with myelodysplastic syndrome (MDS), which was refractory anemia with excess blasts (RAEB). This cell line, designated TER-3, depends on several cytokines for long-term survival and growth, and requires interleukin-3 (IL-3) for continuous growth. Cytochemical analysis revealed that TER-3 cells are weakly dianisidine positive and nonspecific esterase positive, but peroxidase negative. The surface marker profile shows that the TER-3 cells are strongly positive for myeloid, lymphoid, and megakaryocytic antigens such as CD15, CD19, and CD61, and negative for some common multilineage antigens such as CD13, CD33, and CD34. Thus, this cell line has a multilineage phenotype, suggesting that the transformation event occurred in multipotent stem cells. Dianisidine- and nonspecific esterase-positive TER-3 cells increase with granulocyte-colony stimulating factor (G-CSF) rather than with IL-3. These results suggest that the cell line is useful for understanding the mechanism underlying G-CSF-associated hematopoietic cell differentiation and activation in the patient with MDS.

Original languageEnglish
Pages (from-to)183-190
Number of pages8
JournalJournal of Cellular Physiology
Volume191
Issue number2
DOIs
Publication statusPublished - 2002
Externally publishedYes

Fingerprint

Calcium-Calmodulin-Dependent Protein Kinase Type 4
Calcium-Calmodulin-Dependent Protein Kinases
Granulocyte Colony-Stimulating Factor
Down-Regulation
Dianisidine
Cells
Cell Line
Carboxylesterase
Interleukin-3
Myelodysplastic Syndromes
Refractory Anemia with Excess of Blasts
Multipotent Stem Cells
Antigens
Growth
Stem cells
Refractory materials
Peroxidase
Cell Differentiation
Chemical activation
Cytokines

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Cell Biology
  • Physiology

Cite this

New human myelodysplastic cell line, TER-3 : G-CSF specific downregulation of Ca 2+/calmodulin-dependent protein kinase IV. / Mishima, Yuji; Terui, Yasuhito; Mishima, Yuko; Katsuyama, Misa; Mori, Masaki; Tomizuka, Hiroshi; Takizawa, Toshiyuki; Miyazato, Akira; Ueda, Masuzu; Yamada, Muneo; Hayasawa, Hirotoshi; Mizunuma, Nobuyuki; Ishizaka, Yukihito; Ikeda, Kazuma; Kato, Takashi; Ozawa, Keiya; Hatake, Kiyohiko.

In: Journal of Cellular Physiology, Vol. 191, No. 2, 2002, p. 183-190.

Research output: Contribution to journalArticle

Mishima, Y, Terui, Y, Mishima, Y, Katsuyama, M, Mori, M, Tomizuka, H, Takizawa, T, Miyazato, A, Ueda, M, Yamada, M, Hayasawa, H, Mizunuma, N, Ishizaka, Y, Ikeda, K, Kato, T, Ozawa, K & Hatake, K 2002, 'New human myelodysplastic cell line, TER-3: G-CSF specific downregulation of Ca 2+/calmodulin-dependent protein kinase IV', Journal of Cellular Physiology, vol. 191, no. 2, pp. 183-190. https://doi.org/10.1002/jcp.10095
Mishima, Yuji ; Terui, Yasuhito ; Mishima, Yuko ; Katsuyama, Misa ; Mori, Masaki ; Tomizuka, Hiroshi ; Takizawa, Toshiyuki ; Miyazato, Akira ; Ueda, Masuzu ; Yamada, Muneo ; Hayasawa, Hirotoshi ; Mizunuma, Nobuyuki ; Ishizaka, Yukihito ; Ikeda, Kazuma ; Kato, Takashi ; Ozawa, Keiya ; Hatake, Kiyohiko. / New human myelodysplastic cell line, TER-3 : G-CSF specific downregulation of Ca 2+/calmodulin-dependent protein kinase IV. In: Journal of Cellular Physiology. 2002 ; Vol. 191, No. 2. pp. 183-190.
@article{e74da9761ff34c9a8f4ae7df989af64b,
title = "New human myelodysplastic cell line, TER-3: G-CSF specific downregulation of Ca 2+/calmodulin-dependent protein kinase IV",
abstract = "We have established a new hematopoietic cell line from a patient with myelodysplastic syndrome (MDS), which was refractory anemia with excess blasts (RAEB). This cell line, designated TER-3, depends on several cytokines for long-term survival and growth, and requires interleukin-3 (IL-3) for continuous growth. Cytochemical analysis revealed that TER-3 cells are weakly dianisidine positive and nonspecific esterase positive, but peroxidase negative. The surface marker profile shows that the TER-3 cells are strongly positive for myeloid, lymphoid, and megakaryocytic antigens such as CD15, CD19, and CD61, and negative for some common multilineage antigens such as CD13, CD33, and CD34. Thus, this cell line has a multilineage phenotype, suggesting that the transformation event occurred in multipotent stem cells. Dianisidine- and nonspecific esterase-positive TER-3 cells increase with granulocyte-colony stimulating factor (G-CSF) rather than with IL-3. These results suggest that the cell line is useful for understanding the mechanism underlying G-CSF-associated hematopoietic cell differentiation and activation in the patient with MDS.",
author = "Yuji Mishima and Yasuhito Terui and Yuko Mishima and Misa Katsuyama and Masaki Mori and Hiroshi Tomizuka and Toshiyuki Takizawa and Akira Miyazato and Masuzu Ueda and Muneo Yamada and Hirotoshi Hayasawa and Nobuyuki Mizunuma and Yukihito Ishizaka and Kazuma Ikeda and Takashi Kato and Keiya Ozawa and Kiyohiko Hatake",
year = "2002",
doi = "10.1002/jcp.10095",
language = "English",
volume = "191",
pages = "183--190",
journal = "Journal of Cellular Physiology",
issn = "0021-9541",
publisher = "Wiley-Liss Inc.",
number = "2",

}

TY - JOUR

T1 - New human myelodysplastic cell line, TER-3

T2 - G-CSF specific downregulation of Ca 2+/calmodulin-dependent protein kinase IV

AU - Mishima, Yuji

AU - Terui, Yasuhito

AU - Mishima, Yuko

AU - Katsuyama, Misa

AU - Mori, Masaki

AU - Tomizuka, Hiroshi

AU - Takizawa, Toshiyuki

AU - Miyazato, Akira

AU - Ueda, Masuzu

AU - Yamada, Muneo

AU - Hayasawa, Hirotoshi

AU - Mizunuma, Nobuyuki

AU - Ishizaka, Yukihito

AU - Ikeda, Kazuma

AU - Kato, Takashi

AU - Ozawa, Keiya

AU - Hatake, Kiyohiko

PY - 2002

Y1 - 2002

N2 - We have established a new hematopoietic cell line from a patient with myelodysplastic syndrome (MDS), which was refractory anemia with excess blasts (RAEB). This cell line, designated TER-3, depends on several cytokines for long-term survival and growth, and requires interleukin-3 (IL-3) for continuous growth. Cytochemical analysis revealed that TER-3 cells are weakly dianisidine positive and nonspecific esterase positive, but peroxidase negative. The surface marker profile shows that the TER-3 cells are strongly positive for myeloid, lymphoid, and megakaryocytic antigens such as CD15, CD19, and CD61, and negative for some common multilineage antigens such as CD13, CD33, and CD34. Thus, this cell line has a multilineage phenotype, suggesting that the transformation event occurred in multipotent stem cells. Dianisidine- and nonspecific esterase-positive TER-3 cells increase with granulocyte-colony stimulating factor (G-CSF) rather than with IL-3. These results suggest that the cell line is useful for understanding the mechanism underlying G-CSF-associated hematopoietic cell differentiation and activation in the patient with MDS.

AB - We have established a new hematopoietic cell line from a patient with myelodysplastic syndrome (MDS), which was refractory anemia with excess blasts (RAEB). This cell line, designated TER-3, depends on several cytokines for long-term survival and growth, and requires interleukin-3 (IL-3) for continuous growth. Cytochemical analysis revealed that TER-3 cells are weakly dianisidine positive and nonspecific esterase positive, but peroxidase negative. The surface marker profile shows that the TER-3 cells are strongly positive for myeloid, lymphoid, and megakaryocytic antigens such as CD15, CD19, and CD61, and negative for some common multilineage antigens such as CD13, CD33, and CD34. Thus, this cell line has a multilineage phenotype, suggesting that the transformation event occurred in multipotent stem cells. Dianisidine- and nonspecific esterase-positive TER-3 cells increase with granulocyte-colony stimulating factor (G-CSF) rather than with IL-3. These results suggest that the cell line is useful for understanding the mechanism underlying G-CSF-associated hematopoietic cell differentiation and activation in the patient with MDS.

UR - http://www.scopus.com/inward/record.url?scp=18344390536&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=18344390536&partnerID=8YFLogxK

U2 - 10.1002/jcp.10095

DO - 10.1002/jcp.10095

M3 - Article

C2 - 12064461

AN - SCOPUS:18344390536

VL - 191

SP - 183

EP - 190

JO - Journal of Cellular Physiology

JF - Journal of Cellular Physiology

SN - 0021-9541

IS - 2

ER -