New human myelodysplastic cell line, TER-3: G-CSF specific downregulation of Ca 2+/calmodulin-dependent protein kinase IV

Yuji Mishima, Yasuhito Terui, Yuko Mishima, Misa Katsuyama, Masaki Mori, Hiroshi Tomizuka, Toshiyuki Takizawa, Akira Miyazato, Masuzu Ueda, Muneo Yamada, Hirotoshi Hayasawa, Nobuyuki Mizunuma, Yukihito Ishizaka, Kazuma Ikeda, Takashi Kato, Keiya Ozawa, Kiyohiko Hatake

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

We have established a new hematopoietic cell line from a patient with myelodysplastic syndrome (MDS), which was refractory anemia with excess blasts (RAEB). This cell line, designated TER-3, depends on several cytokines for long-term survival and growth, and requires interleukin-3 (IL-3) for continuous growth. Cytochemical analysis revealed that TER-3 cells are weakly dianisidine positive and nonspecific esterase positive, but peroxidase negative. The surface marker profile shows that the TER-3 cells are strongly positive for myeloid, lymphoid, and megakaryocytic antigens such as CD15, CD19, and CD61, and negative for some common multilineage antigens such as CD13, CD33, and CD34. Thus, this cell line has a multilineage phenotype, suggesting that the transformation event occurred in multipotent stem cells. Dianisidine- and nonspecific esterase-positive TER-3 cells increase with granulocyte-colony stimulating factor (G-CSF) rather than with IL-3. These results suggest that the cell line is useful for understanding the mechanism underlying G-CSF-associated hematopoietic cell differentiation and activation in the patient with MDS.

Original languageEnglish
Pages (from-to)183-190
Number of pages8
JournalJournal of Cellular Physiology
Volume191
Issue number2
DOIs
Publication statusPublished - 2002 Apr 15
Externally publishedYes

ASJC Scopus subject areas

  • Physiology
  • Clinical Biochemistry
  • Cell Biology

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  • Cite this

    Mishima, Y., Terui, Y., Mishima, Y., Katsuyama, M., Mori, M., Tomizuka, H., Takizawa, T., Miyazato, A., Ueda, M., Yamada, M., Hayasawa, H., Mizunuma, N., Ishizaka, Y., Ikeda, K., Kato, T., Ozawa, K., & Hatake, K. (2002). New human myelodysplastic cell line, TER-3: G-CSF specific downregulation of Ca 2+/calmodulin-dependent protein kinase IV. Journal of Cellular Physiology, 191(2), 183-190. https://doi.org/10.1002/jcp.10095