New L-Amino acid ligases catalyzing oligopeptide synthesis from various microorganisms

Toshinobu Arai, Kuniki Kino

    Research output: Contribution to journalArticle

    13 Citations (Scopus)

    Abstract

    L-Amino acid ligase synthesizes various peptides from unprotected L-amino acids in an ATP-dependent manner. Known L-amino acid ligases catalyze only dipeptide synthesis, but recently we found that RizB of Bacillus subtilis NBRC 3134 catalyzes oligopeptide synthesis. In the present study, we searched for new members of the L-amino acid ligase group that catalyze oligopeptide synthesis. Several hypothetical proteins possessing the ATP-grasp motif were selected by in silico analysis. These recombinant proteins were assayed for L-amino acid ligase activity. We obtained five L-amino acid ligases showing oligopeptide synthesis activities. These proteins showed low similarity in amino acid sequence, but commonly used branched-chain amino acids, such as RizB, as substrates. Furthermore, the spr0969 protein of Streptococcus pneumoniae synthesized longer peptides than those synthesized by RizB, and the BAD 1200 protein of Bifidobacterium adolescentis showed higher activity toward aromatic amino acids than toward branched-chain ones. We also examined some of their characteristics.

    Original languageEnglish
    Pages (from-to)1572-1577
    Number of pages6
    JournalBioscience, Biotechnology and Biochemistry
    Volume74
    Issue number8
    DOIs
    Publication statusPublished - 2010

    Fingerprint

    Oligopeptides
    Ligases
    Microorganisms
    Amino Acids
    Proteins
    Adenosine Triphosphate
    Branched Chain Amino Acids
    Aromatic Amino Acids
    Dipeptides
    Hand Strength
    Streptococcus pneumoniae
    Bacillus subtilis
    Recombinant Proteins
    Computer Simulation
    Bacilli
    Amino Acid Sequence
    Peptides
    Substrates

    Keywords

    • In silico analysis
    • L-amino acid ligase
    • Oligopeptide
    • Peptide synthesis

    ASJC Scopus subject areas

    • Biotechnology
    • Biochemistry
    • Molecular Biology
    • Applied Microbiology and Biotechnology
    • Analytical Chemistry
    • Organic Chemistry

    Cite this

    New L-Amino acid ligases catalyzing oligopeptide synthesis from various microorganisms. / Arai, Toshinobu; Kino, Kuniki.

    In: Bioscience, Biotechnology and Biochemistry, Vol. 74, No. 8, 2010, p. 1572-1577.

    Research output: Contribution to journalArticle

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