Newt prolactin and its involvement in reproduction

S. Kikuyama, T. Yazawa, S. Abe, K. Yamamoto, T. Iwata, K. Hoshi, I. Hasunuma, G. Mosconi, A. M. Polzonetti-Magni

    Research output: Contribution to journalArticle

    22 Citations (Scopus)

    Abstract

    The amino acid sequence of newt (Cynops pyrrhogaster) prolactin deduced from the nucleotide sequence of its cDNA showed a relatively high homology with sequences of chicken and sea turtle prolactins as well as with those of anuran prolactins. Cynops prolactin receptor transcripts were detected in various tissues and organs, suggesting that prolactin plays multiple roles in urodeles. Urodele prolactin was purified from the pituitaries of C. pyrrhogaster. Antiserum against this prolactin was used for radioimmunoassay of plasma prolactin and immunoneutralization experiments. Endogenous prolactin was shown to induce migration to water, courtship behavior, and cessation of spermatocytogenesis in the Cynops newt. The hormone was found to be involved in the development of cloacal glands such as the lateral and abdominal glands, growth of the tail and Mauthner neurons, secretion of oviducal jelly, and enhanced synthesis of a female attracting pheromone (sodefrin), and responsiveness of the olfactory epithelium to sodefrin. In most of these cases, prolactin was found to act synergistically or antagonistically with sex steroids. We also discovered that hypersecretion of prolactin in the newts subjected to cold temperature was induced by hypothalamic stimulation rather than release from hypothalamic inhibition.

    Original languageEnglish
    Pages (from-to)984-993
    Number of pages10
    JournalCanadian Journal of Physiology and Pharmacology
    Volume78
    Issue number12
    DOIs
    Publication statusPublished - 2000

    Fingerprint

    Salamandridae
    Prolactin
    Reproduction
    sodefrin
    Prolactin Receptors
    Courtship
    Olfactory Mucosa
    Turtles
    Pheromones
    Spermatogenesis
    Sequence Homology
    Radioimmunoassay
    Tail
    Immune Sera
    Amino Acid Sequence
    Chickens
    Complementary DNA
    Steroids
    Hormones

    Keywords

    • Newts
    • Prolactin
    • Reproduction

    ASJC Scopus subject areas

    • Physiology
    • Pharmacology

    Cite this

    Kikuyama, S., Yazawa, T., Abe, S., Yamamoto, K., Iwata, T., Hoshi, K., ... Polzonetti-Magni, A. M. (2000). Newt prolactin and its involvement in reproduction. Canadian Journal of Physiology and Pharmacology, 78(12), 984-993. https://doi.org/10.1139/cjpp-78-12-984

    Newt prolactin and its involvement in reproduction. / Kikuyama, S.; Yazawa, T.; Abe, S.; Yamamoto, K.; Iwata, T.; Hoshi, K.; Hasunuma, I.; Mosconi, G.; Polzonetti-Magni, A. M.

    In: Canadian Journal of Physiology and Pharmacology, Vol. 78, No. 12, 2000, p. 984-993.

    Research output: Contribution to journalArticle

    Kikuyama, S, Yazawa, T, Abe, S, Yamamoto, K, Iwata, T, Hoshi, K, Hasunuma, I, Mosconi, G & Polzonetti-Magni, AM 2000, 'Newt prolactin and its involvement in reproduction', Canadian Journal of Physiology and Pharmacology, vol. 78, no. 12, pp. 984-993. https://doi.org/10.1139/cjpp-78-12-984
    Kikuyama S, Yazawa T, Abe S, Yamamoto K, Iwata T, Hoshi K et al. Newt prolactin and its involvement in reproduction. Canadian Journal of Physiology and Pharmacology. 2000;78(12):984-993. https://doi.org/10.1139/cjpp-78-12-984
    Kikuyama, S. ; Yazawa, T. ; Abe, S. ; Yamamoto, K. ; Iwata, T. ; Hoshi, K. ; Hasunuma, I. ; Mosconi, G. ; Polzonetti-Magni, A. M. / Newt prolactin and its involvement in reproduction. In: Canadian Journal of Physiology and Pharmacology. 2000 ; Vol. 78, No. 12. pp. 984-993.
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