Nitric oxide generation around buccal ganglia accompanying feeding behavior in the pond snail, Lymnaea stagnalis

Suguru Kobayashi, Hisayo Sadamoto, Hiroto Ogawa, Yoshiichiro Kitamura, Kotaro Oka, Kazuo Tanishita, Etsuro Ito

Research output: Contribution to journalArticle

45 Citations (Scopus)

Abstract

Although there are many lines of evidence for both the presence of nitric oxide synthase (NOS) in the central nervous system (CNS) and the effects of NO on activating and modulating the feeding circuit in Lymnaea stagnalis, there has been no direct evidence that NO generation in the CNS accompanies feeding behavior. In the present study, we used a NO specific electrode to measure the increase in NO concentration around the buccal ganglia when the lips of semi-intact preparations of L. stagnalis were stimulated by sucrose. The NO concentration of the buccal ganglia was significantly increased by an application of sucrose to the lips. A NO scavenger and a NOS inhibitor suppressed this increase in NO concentration. A pair of putative NO-generative neurons in the buccal ganglia, the B2 cells, are active during the inter-feeding phase, and the bursting of the B2 cell elicited by sucrose application starts simultaneously with the feeding response. The rhythmic pulses of NO generation corresponded well with the rhythmic bursting of the B2 cells, which itself corresponds to the 'fictive feeding response'. The present data provide the first direct evidence that NO is generated in the buccal ganglia of L. staganalis and is involved in a specific behavior such as feeding. (C) 2000 Elsevier Science Ireland Ltd.

Original languageEnglish
Pages (from-to)27-34
Number of pages8
JournalNeuroscience Research
Volume38
Issue number1
DOIs
Publication statusPublished - 2000 Sep 1
Externally publishedYes

Fingerprint

Lymnaea
Cheek
Snails
Feeding Behavior
Ganglia
Nitric Oxide
Sucrose
Lip
Nitric Oxide Synthase
Central Nervous System
Electrodes
Neurons

Keywords

  • Feeding behavior
  • Lymnaea stagnalis
  • Nitric Oxide
  • NO electrode

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Nitric oxide generation around buccal ganglia accompanying feeding behavior in the pond snail, Lymnaea stagnalis. / Kobayashi, Suguru; Sadamoto, Hisayo; Ogawa, Hiroto; Kitamura, Yoshiichiro; Oka, Kotaro; Tanishita, Kazuo; Ito, Etsuro.

In: Neuroscience Research, Vol. 38, No. 1, 01.09.2000, p. 27-34.

Research output: Contribution to journalArticle

Kobayashi, Suguru ; Sadamoto, Hisayo ; Ogawa, Hiroto ; Kitamura, Yoshiichiro ; Oka, Kotaro ; Tanishita, Kazuo ; Ito, Etsuro. / Nitric oxide generation around buccal ganglia accompanying feeding behavior in the pond snail, Lymnaea stagnalis. In: Neuroscience Research. 2000 ; Vol. 38, No. 1. pp. 27-34.
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