Nitric oxide synthases and soluble guanylyl cyclase underlying the modulation of electrical oscillations in a central olfactory organ

Sayoko Fujie, Takehiro Yamamoto, Jun Murakami, Dai Hatakeyama, Katsuki Shiga, Norio Suzuki, Etsuro Ito

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

We have isolated and characterized the cDNAs for nitric oxide synthase (NOS) and soluble guanylyl cyclase (sGC) from the terrestrial slug Limax marginatus, and examined the presence and distribution of their mRNAs in the central nervous system using histological techniques and a reverse transcription-polymerase chain reaction method. Our results showed that both bursting and nonbursting neurons in the procerebral lobes contain the mRNAs for both NOS and sGC. We further found that the oscillation frequency of electrical activity in the procerebral lobes increases with increasing intracellular concentrations of cyclic GMP (cGMP). Taken together with previous data on the NO-induced cGMP-like immunoreactivity and on the anatomical distribution of neuntes and the localization of synapses of bursting and nonborstieg neurons, our present results suggest that NO-induced changes in cGMP concentration modulate the oscillation frequency in the procerebral lobes by acting on the olfactory input pathways, but possibly not on the output pathways, in slugs.

Original languageEnglish
Pages (from-to)14-30
Number of pages17
JournalJournal of Neurobiology
Volume62
Issue number1
DOIs
Publication statusPublished - 2005 Jan
Externally publishedYes

Fingerprint

Cyclic GMP
Nitric Oxide Synthase
Gastropoda
Histological Techniques
Neurons
Olfactory Pathways
Messenger RNA
Synapses
Reverse Transcription
Central Nervous System
Complementary DNA
Polymerase Chain Reaction
Soluble Guanylyl Cyclase

Keywords

  • Cyclic GMP
  • Mollusk
  • Nitric oxide
  • Olfaction
  • Procerebral lobe

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Nitric oxide synthases and soluble guanylyl cyclase underlying the modulation of electrical oscillations in a central olfactory organ. / Fujie, Sayoko; Yamamoto, Takehiro; Murakami, Jun; Hatakeyama, Dai; Shiga, Katsuki; Suzuki, Norio; Ito, Etsuro.

In: Journal of Neurobiology, Vol. 62, No. 1, 01.2005, p. 14-30.

Research output: Contribution to journalArticle

Fujie, Sayoko ; Yamamoto, Takehiro ; Murakami, Jun ; Hatakeyama, Dai ; Shiga, Katsuki ; Suzuki, Norio ; Ito, Etsuro. / Nitric oxide synthases and soluble guanylyl cyclase underlying the modulation of electrical oscillations in a central olfactory organ. In: Journal of Neurobiology. 2005 ; Vol. 62, No. 1. pp. 14-30.
@article{49c71a24d78c4393baf9a76498b4c543,
title = "Nitric oxide synthases and soluble guanylyl cyclase underlying the modulation of electrical oscillations in a central olfactory organ",
abstract = "We have isolated and characterized the cDNAs for nitric oxide synthase (NOS) and soluble guanylyl cyclase (sGC) from the terrestrial slug Limax marginatus, and examined the presence and distribution of their mRNAs in the central nervous system using histological techniques and a reverse transcription-polymerase chain reaction method. Our results showed that both bursting and nonbursting neurons in the procerebral lobes contain the mRNAs for both NOS and sGC. We further found that the oscillation frequency of electrical activity in the procerebral lobes increases with increasing intracellular concentrations of cyclic GMP (cGMP). Taken together with previous data on the NO-induced cGMP-like immunoreactivity and on the anatomical distribution of neuntes and the localization of synapses of bursting and nonborstieg neurons, our present results suggest that NO-induced changes in cGMP concentration modulate the oscillation frequency in the procerebral lobes by acting on the olfactory input pathways, but possibly not on the output pathways, in slugs.",
keywords = "Cyclic GMP, Mollusk, Nitric oxide, Olfaction, Procerebral lobe",
author = "Sayoko Fujie and Takehiro Yamamoto and Jun Murakami and Dai Hatakeyama and Katsuki Shiga and Norio Suzuki and Etsuro Ito",
year = "2005",
month = "1",
doi = "10.1002/neu.20046",
language = "English",
volume = "62",
pages = "14--30",
journal = "Developmental Neurobiology",
issn = "1932-8451",
publisher = "John Wiley and Sons Inc.",
number = "1",

}

TY - JOUR

T1 - Nitric oxide synthases and soluble guanylyl cyclase underlying the modulation of electrical oscillations in a central olfactory organ

AU - Fujie, Sayoko

AU - Yamamoto, Takehiro

AU - Murakami, Jun

AU - Hatakeyama, Dai

AU - Shiga, Katsuki

AU - Suzuki, Norio

AU - Ito, Etsuro

PY - 2005/1

Y1 - 2005/1

N2 - We have isolated and characterized the cDNAs for nitric oxide synthase (NOS) and soluble guanylyl cyclase (sGC) from the terrestrial slug Limax marginatus, and examined the presence and distribution of their mRNAs in the central nervous system using histological techniques and a reverse transcription-polymerase chain reaction method. Our results showed that both bursting and nonbursting neurons in the procerebral lobes contain the mRNAs for both NOS and sGC. We further found that the oscillation frequency of electrical activity in the procerebral lobes increases with increasing intracellular concentrations of cyclic GMP (cGMP). Taken together with previous data on the NO-induced cGMP-like immunoreactivity and on the anatomical distribution of neuntes and the localization of synapses of bursting and nonborstieg neurons, our present results suggest that NO-induced changes in cGMP concentration modulate the oscillation frequency in the procerebral lobes by acting on the olfactory input pathways, but possibly not on the output pathways, in slugs.

AB - We have isolated and characterized the cDNAs for nitric oxide synthase (NOS) and soluble guanylyl cyclase (sGC) from the terrestrial slug Limax marginatus, and examined the presence and distribution of their mRNAs in the central nervous system using histological techniques and a reverse transcription-polymerase chain reaction method. Our results showed that both bursting and nonbursting neurons in the procerebral lobes contain the mRNAs for both NOS and sGC. We further found that the oscillation frequency of electrical activity in the procerebral lobes increases with increasing intracellular concentrations of cyclic GMP (cGMP). Taken together with previous data on the NO-induced cGMP-like immunoreactivity and on the anatomical distribution of neuntes and the localization of synapses of bursting and nonborstieg neurons, our present results suggest that NO-induced changes in cGMP concentration modulate the oscillation frequency in the procerebral lobes by acting on the olfactory input pathways, but possibly not on the output pathways, in slugs.

KW - Cyclic GMP

KW - Mollusk

KW - Nitric oxide

KW - Olfaction

KW - Procerebral lobe

UR - http://www.scopus.com/inward/record.url?scp=10444265330&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=10444265330&partnerID=8YFLogxK

U2 - 10.1002/neu.20046

DO - 10.1002/neu.20046

M3 - Article

VL - 62

SP - 14

EP - 30

JO - Developmental Neurobiology

JF - Developmental Neurobiology

SN - 1932-8451

IS - 1

ER -