Nitrification of landfill leachate using immobilized nitrifying bacteria at low temperatures

Kazuichi Isaka, Sachiko Yoshie, Tatsuo Sumino, Yuhei Inamori, Satoshi Tsuneda

    Research output: Contribution to journalArticle

    50 Citations (Scopus)

    Abstract

    A technology to achieve stable and high rates of nitrification of landfill leachate at low temperatures has been desired. Nitrifying bacteria entrapped in a polyethylene glycol (PEG) gel carrier produced high nitrification rates of 0.71 kg N/m3/day at 10 °C for more than 1 year. As a characteristic of nitrification, ammonium nitrogen at 16-35 mg/L remained in effluent water irrespective of nitrogen load and nitrite accumulation was observed. Batch experiments clearly showed that the relationship between ammonium concentration and ammonium removal rate followed a Monod-type equation. It was also revealed that ammonium-oxidizing bacteria cultivated in a gel carrier had a low affinity for ammonium, leading to incomplete nitrification. Moreover, it was suggested that the remaining ammonium in the reactor produced free ammonium, which inhibited the activities of nitrite-oxidizing bacteria. Thus, only nitritation was observed. Molecular biological methods, such as denaturing gradient gel electrophoresis (DGGE) and fluorescence in situ hybridization (FISH), revealed that Nitrosomonas sp. was the dominant ammonium-oxidizing bacteria in the gel carrier at low temperature.

    Original languageEnglish
    Pages (from-to)49-55
    Number of pages7
    JournalBiochemical Engineering Journal
    Volume37
    Issue number1
    DOIs
    Publication statusPublished - 2007 Oct 15

    Fingerprint

    Chemical Water Pollutants
    Nitrification
    Land fill
    Ammonium Compounds
    Bacteria
    Gels
    Temperature
    Nitrogen
    Nitrites
    Electrophoresis
    Polyethylene glycols
    Effluents
    Nitrosomonas
    Fluorescence
    Denaturing Gradient Gel Electrophoresis
    Fluorescence In Situ Hybridization
    Water
    Experiments
    Technology

    Keywords

    • Aerobic processes
    • Bioreactor
    • Immobilization
    • Leachate
    • Nitrification
    • Wastewater treatment

    ASJC Scopus subject areas

    • Biotechnology
    • Bioengineering
    • Chemical Engineering(all)

    Cite this

    Nitrification of landfill leachate using immobilized nitrifying bacteria at low temperatures. / Isaka, Kazuichi; Yoshie, Sachiko; Sumino, Tatsuo; Inamori, Yuhei; Tsuneda, Satoshi.

    In: Biochemical Engineering Journal, Vol. 37, No. 1, 15.10.2007, p. 49-55.

    Research output: Contribution to journalArticle

    Isaka, Kazuichi ; Yoshie, Sachiko ; Sumino, Tatsuo ; Inamori, Yuhei ; Tsuneda, Satoshi. / Nitrification of landfill leachate using immobilized nitrifying bacteria at low temperatures. In: Biochemical Engineering Journal. 2007 ; Vol. 37, No. 1. pp. 49-55.
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