Nitrogen fixation by immobilized Azotobacter chroococcum

Isao Karube, Tadashi Matsunaga, Yasuo Otomine, Shuichi Suzuki

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

A nitrogen-fixing bacterium, Azotobacter chroococcum, was immobilized in 2% agar gel. The optimum partial oxygen pressure, pO2, of immobilized cells was 0.2 atm, wherea s that of native cells was 0.05 atm. When continual nitrogen fixation was performed under aerobic conditions, the nitrogenase activity of immobilized cells increased with increasing time. On the other hand, the activity of native cells decreased rapidly. Increase of nitrogenase activity was attributed to growth of the bacteria in the gel matrix. The production rate of total nitrogen compounds by the immobilized bacteria was also increased during the first 4 days. Nitrogen compounds produced by the immobilized cells were mainly amino acids such as γ-aminobutyrate, glutamate and arginine.

Original languageEnglish
Pages (from-to)309-312
Number of pages4
JournalEnzyme and Microbial Technology
Volume3
Issue number4
DOIs
Publication statusPublished - 1981
Externally publishedYes

Fingerprint

Azotobacter
Nitrogen fixation
Immobilized Cells
Nitrogen Fixation
Nitrogen Compounds
Nitrogenase
Cells
arginine glutamate
Nitrogen compounds
Bacteria
Gels
Aminobutyrates
Partial Pressure
Arginine
Agar
Oxygen
Amino acids
Amino Acids
Nitrogen
Growth

ASJC Scopus subject areas

  • Biochemistry
  • Biotechnology
  • Applied Microbiology and Biotechnology

Cite this

Nitrogen fixation by immobilized Azotobacter chroococcum. / Karube, Isao; Matsunaga, Tadashi; Otomine, Yasuo; Suzuki, Shuichi.

In: Enzyme and Microbial Technology, Vol. 3, No. 4, 1981, p. 309-312.

Research output: Contribution to journalArticle

Karube, I, Matsunaga, T, Otomine, Y & Suzuki, S 1981, 'Nitrogen fixation by immobilized Azotobacter chroococcum', Enzyme and Microbial Technology, vol. 3, no. 4, pp. 309-312. https://doi.org/10.1016/0141-0229(81)90005-3
Karube, Isao ; Matsunaga, Tadashi ; Otomine, Yasuo ; Suzuki, Shuichi. / Nitrogen fixation by immobilized Azotobacter chroococcum. In: Enzyme and Microbial Technology. 1981 ; Vol. 3, No. 4. pp. 309-312.
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